Actividad in-vitro de microorganismos autóctonos (Bacillus subtilis y Lactobacillus brevis) para reducir la colonización de salmonella entérica
The in-vitro activity of autochthonous microorganisms (Lactobacillus brevis, Bacillus subtilis and the mixture of both) was determined to reduce the colonization of enteric Salmonella. To this end, a DCA with a factorial arrangement was used, in the inhibition of the confrontation of microorganisms,...
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| Format: | bachelorThesis |
| Jezik: | spa |
| Izdano: |
2019
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| Online dostop: | http://repositorio.espam.edu.ec/handle/42000/1149 |
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Označite | Izvleček: | The in-vitro activity of autochthonous microorganisms (Lactobacillus brevis, Bacillus subtilis and the mixture of both) was determined to reduce the colonization of enteric Salmonella. To this end, a DCA with a factorial arrangement was used, in the inhibition of the confrontation of microorganisms, 9 treatments were used with 4 repetitions by the Kirby-Bauer technique; In the survival of bile salts, 4 treatments and 12 repetitions were used by counting CFU and in the analysis of the growth curve was determined by descriptive statistics by absorbance with a UV spectrophotometer with a wavelength of 560 nm and a factor of 0.9. The microorganisms with application of 3 methods against enteric Salmonella had a favorable growth at 24 hours, obtaining a greater development with Lactobacillus brevis + Bacillus subtilis by filtration method. In the growth curve, Lactobacillus brevis had a greater development at hour 20, with a decline of between 24 to 32 hours, while Bacillus subtilis had a greater growth at 24 hours, decreasing its activity at 28 and 32 hours staying above Lactobacillus brevis. In the survival to bile salts, no variability of results was found, however, the Lactobacillus brevis without bile salts stood out from minute 30 until minute 120. It is concluded that both microorganisms meet physical and biological characteristics that would make them suitable candidates to be used as probiotics in animal feed for animal production purposes. |
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