Agentes causales de mastitis en bovinos positivos de ganaderías adscritas a centro de acopio de lácteos y antibiorresistencia
This study was carried out on farms affiliated with the dairy collection center of the San Isidro parish, Cantón Sucre, with the objective of evaluating animals that present subclinical mastitis infection, for this, California Mastitis Test (CMT) tests were carried out in the field. ) and dark backg...
-д хадгалсан:
| Үндсэн зохиолч: | |
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| Бусад зохиолчид: | |
| Формат: | bachelorThesis |
| Хэл сонгох: | spa |
| Хэвлэсэн: |
2023
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| Нөхцлүүд: | |
| Онлайн хандалт: | http://repositorio.espam.edu.ec/handle/42000/2289 |
| Шошгууд: |
Шошго нэмэх
Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!
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Шошго нэмэх | Тойм: | This study was carried out on farms affiliated with the dairy collection center of the San Isidro parish, Cantón Sucre, with the objective of evaluating animals that present subclinical mastitis infection, for this, California Mastitis Test (CMT) tests were carried out in the field. ) and dark background in milk from the cows involved, laboratory tests were subsequently carried out in order to identify etiological agents exclusive to the area studied; A tube coagulase test was performed, inoculated in Mannitol Salt Agar, with the use of rabbit plasma, and they were reviewed at 4, 18 and 24 hours. Additionally, miniaturized Biomérieux-API® systems (ID32-Mexico) were applied as rapid methods to discriminate or detect causal agents by performing different biochemical tests. Initially, each microtube of the system was inoculated with a suspension in 0.85% saline solution of pure culture of the microorganism to be identified. Once the gallery was inoculated with the suspension of the microorganism to be identified, the system required to be incubated in a large anaerobiosis jar complete with 9.5 Liters (Gaspak 150 - United States) under anaerobiosis conditions. The following etiological agents were found: Staphylococcus Epidermidis (52.9%), Staphylococcus Aureus (29.4%) and Streptococcus Uberis (17.6%). After the isolation and identification of the causal agents for bovine mastitis, their resistance to the following antimicrobials was analyzed using sensidisks: Cephalexin, Oxytetracycline, Cloxacillin, Streptomycin and Cephalosporin, antibiotics that were highly effective against the etiological agents found. The data processing analysis was carried out using the Excel 365 computer program. |
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