Efecto de dos diluyentes y tiempo de permanencia sobre la viabilidad del semen de bovinos mestizos lecheros

The objective of this research was to evaluate the effect of two diluents and residence time on the viability of semen from crossbred dairy cattle. For this, semen was collected from three breeding bulls of the Gyropar breed (Gyr x Pardo Suizo), 36 months old. The treatments were organized in a DCA design with a factorial arrangement (3x2), these being: T1: Toro 1 with Optixcell®; T2: Bull 1 with Andromed®; T3: Toro 2 with Optixcell®; T4: Toro 2 with Andromed®; T5: Toro 3 with Optixcell®; T6: Toro 3 with Andromed®. The variables to measure were: motility, normality and vitality at 0, 12 and 24 hours, while Normal Apical Edge (NAR), Damaged Apical Edge (DAR), Cytoplasmic Drop (GCs) in fresh semen. The results obtained were: in motility (p <0.05), hour 0 stood out in T1 (91.25 ± 2.50). Normality (p <0.05), a higher average was obtained at 0 hours on T1 (82.50 ± 6.57). While in vitality (p <0.05) the highest average was reached in T1 (97.00 ± 0.82). The morphological variables presented statistical differences (p <0.05); it was highlighted in the NAR variables in T3 (94.00 ± 2.58); GIVE T4 (8.75 ± 2.99) and GCs T6 (12.75 ± 4.43). It is concluded that the Andromed® and Optixcell® implementation does not have negative effects on the morphological quality parameters and the structural and functional integrity of the plasma and acrosomal membrane of the semen.

• 主要作者: Andrade Castro, Gabriel Andrés (author)
• 其他作者: Palma Andrade, Roberto Carlos (author)
• 格式: bachelorThesis
• 語言: spa
• 出版: 2020
• 主題: Andromed®; Optixcell®; Borde Apical Normal (NAR); Borde Apical Dañado (DAR); Gota Citoplasmática (GCs)
• 在線閱讀: http://repositorio.espam.edu.ec/handle/42000/1302