Evaluación de Bacillus sp. antárticos en la biodegradación de polietileno PET
The objective of this study was to evaluate the biodegradation of polyethylene terephthalate (PET) by means of Bacillus sp. isolated from Antarctica. This research was of an experimental type, scientific approach on a unifactorial Completely Random Block Design (RCBD) and three blocks (15, 30 and 45...
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| Format: | bachelorThesis |
| Langue: | spa |
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2023
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| Accès en ligne: | http://repositorio.espam.edu.ec/handle/42000/2084 |
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| author | Álava Cevallos, Sállury Gema |
| author2 | Velásquez Catagua, Claribel Accidalia |
| author2_role | author |
| author_facet | Álava Cevallos, Sállury Gema Velásquez Catagua, Claribel Accidalia |
| author_role | author |
| collection | Repositorio Escuela Superior Politécnica Agropecuaria de Manabí |
| dc.contributor.none.fl_str_mv | Pincay Cantos, María Fernanda |
| dc.creator.none.fl_str_mv | Álava Cevallos, Sállury Gema Velásquez Catagua, Claribel Accidalia |
| dc.date.none.fl_str_mv | 2023-03-17T20:35:51Z 2023-03-17T20:35:51Z 2023-02 |
| dc.format.none.fl_str_mv | 67 p. application/pdf |
| dc.identifier.none.fl_str_mv | http://repositorio.espam.edu.ec/handle/42000/2084 |
| dc.language.none.fl_str_mv | spa |
| dc.publisher.none.fl_str_mv | Calceta: ESPAM MFL |
| dc.rights.none.fl_str_mv | Atribución-NoComercial-SinDerivadas 3.0 Ecuador http://creativecommons.org/licenses/by-nc-nd/3.0/ec/ info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Escuela Superior Politécnica Agropecuaria de Manabí instname:Escuela Superior Politécnica Agropecuaria de Manabí instacron:ESPAM |
| dc.subject.none.fl_str_mv | Polietileno tereftalato Bacillus sp. Biodegradación Estructura polimérica |
| dc.title.none.fl_str_mv | Evaluación de Bacillus sp. antárticos en la biodegradación de polietileno PET |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
| description | The objective of this study was to evaluate the biodegradation of polyethylene terephthalate (PET) by means of Bacillus sp. isolated from Antarctica. This research was of an experimental type, scientific approach on a unifactorial Completely Random Block Design (RCBD) and three blocks (15, 30 and 45 days). As treatments, five strains of the genus Bacillus sp. (DEE-71, T-29, T-13, GA-52, T-21) and control. The main material was the PET sheets that were subjected to a treatment with the bacteria in an enriched and mineral medium under room temperature conditions of 20°C and at 180 rpm. The biodegradation was evaluated by means of the percentage of weight loss and observation by light microscopy for two months. In the same way, the data were taken to an analysis of variance (ANOVA) and Tukey's test with 95% confidence. The percentage of biodegradation of the PET that will continue after 45 days was 0.4000%; 0.4250%; 0.0750%; 0.3250%; 0.2750%; 0.0000%, respectively, for the above-mentioned strains and the control. Likewise, signs of biodegradation were verified in the PET samples, such as: deterioration, fissures and grooves. Finally, the results of the present study revealed changes in the polymeric structure through the biodegrading capacity of the bacteria. |
| eu_rights_str_mv | openAccess |
| format | bachelorThesis |
| id | ESPAM_7d2e4e1ad1486c55980ff978197390a1 |
| instacron_str | ESPAM |
| institution | ESPAM |
| instname_str | Escuela Superior Politécnica Agropecuaria de Manabí |
| language | spa |
| network_acronym_str | ESPAM |
| network_name_str | Repositorio Escuela Superior Politécnica Agropecuaria de Manabí |
| oai_identifier_str | oai:null:42000/2084 |
| publishDate | 2023 |
| publisher.none.fl_str_mv | Calceta: ESPAM MFL |
| reponame_str | Repositorio Escuela Superior Politécnica Agropecuaria de Manabí |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Repositorio Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabí |
| repository_id_str | 0 |
| rights_invalid_str_mv | Atribución-NoComercial-SinDerivadas 3.0 Ecuador http://creativecommons.org/licenses/by-nc-nd/3.0/ec/ |
| spelling | Evaluación de Bacillus sp. antárticos en la biodegradación de polietileno PETÁlava Cevallos, Sállury GemaVelásquez Catagua, Claribel AccidaliaPolietileno tereftalatoBacillus sp.BiodegradaciónEstructura poliméricaThe objective of this study was to evaluate the biodegradation of polyethylene terephthalate (PET) by means of Bacillus sp. isolated from Antarctica. This research was of an experimental type, scientific approach on a unifactorial Completely Random Block Design (RCBD) and three blocks (15, 30 and 45 days). As treatments, five strains of the genus Bacillus sp. (DEE-71, T-29, T-13, GA-52, T-21) and control. The main material was the PET sheets that were subjected to a treatment with the bacteria in an enriched and mineral medium under room temperature conditions of 20°C and at 180 rpm. The biodegradation was evaluated by means of the percentage of weight loss and observation by light microscopy for two months. In the same way, the data were taken to an analysis of variance (ANOVA) and Tukey's test with 95% confidence. The percentage of biodegradation of the PET that will continue after 45 days was 0.4000%; 0.4250%; 0.0750%; 0.3250%; 0.2750%; 0.0000%, respectively, for the above-mentioned strains and the control. Likewise, signs of biodegradation were verified in the PET samples, such as: deterioration, fissures and grooves. Finally, the results of the present study revealed changes in the polymeric structure through the biodegrading capacity of the bacteria.El objetivo del presente estudio fue evaluar la biodegradación de polietileno tereftalato (PET) por medio de Bacillus sp. aislados de la Antártida. Esta investigación fue de tipo experimental, enfoque científico sobre un Diseño de Bloques Completamente al Azar (DBCA) unifactorial y tres bloques (15, 30 y 45 días). Como tratamientos se consideraron cinco cepas del género Bacillus sp. (DEE-71, T-29, T-13, GA-52, T-21) y control. El material principal fueron las láminas de PET que se sometieron a tratamiento con las bacterias en medio enriquecido y mineral en condiciones de temperatura ambiente de 20°C y a 180 rpm. La biodegradación se evaluó mediante el porcentaje de pérdida de peso y observación por microscopía óptica durante 2 meses. Del mismo modo, los datos se llevaron a un análisis de varianza (ANOVA) y test de Tukey con 95% de confianza. El porcentaje de biodegradación del PET que se determinó después de 45 días fue 0.4000%; 0.4250%; 0.0750%; 0.3250%; 0.2750%; 0.0000%, respectivamente, para las cepas anteriormente mencionadas y el control. Asimismo, se comprobó indicios de biodegradación en las muestras de PET, tales como: deterioro, fisuras y surcos. Finalmente, los resultados del presente estudio revelaron cambios sobre la estructura polimérica mediante la capacidad biodegradadora de las bacterias.Calceta: ESPAM MFLPincay Cantos, María Fernanda2023-03-17T20:35:51Z2023-03-17T20:35:51Z2023-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesis67 p.application/pdfhttp://repositorio.espam.edu.ec/handle/42000/2084spaAtribución-NoComercial-SinDerivadas 3.0 Ecuadorhttp://creativecommons.org/licenses/by-nc-nd/3.0/ec/info:eu-repo/semantics/openAccessreponame:Repositorio Escuela Superior Politécnica Agropecuaria de Manabíinstname:Escuela Superior Politécnica Agropecuaria de Manabíinstacron:ESPAM2025-12-30T06:49:18Zoai:null:42000/2084Institucionalhttps://repositorio.espam.edu.ec/Universidad públicahttp://www.espam.edu.ec/https://repositorio.espam.edu.ec/oai.Ecuador...opendoar:02025-12-30T06:49:18Repositorio Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabífalse |
| spellingShingle | Evaluación de Bacillus sp. antárticos en la biodegradación de polietileno PET Álava Cevallos, Sállury Gema Polietileno tereftalato Bacillus sp. Biodegradación Estructura polimérica |
| status_str | publishedVersion |
| title | Evaluación de Bacillus sp. antárticos en la biodegradación de polietileno PET |
| title_full | Evaluación de Bacillus sp. antárticos en la biodegradación de polietileno PET |
| title_fullStr | Evaluación de Bacillus sp. antárticos en la biodegradación de polietileno PET |
| title_full_unstemmed | Evaluación de Bacillus sp. antárticos en la biodegradación de polietileno PET |
| title_short | Evaluación de Bacillus sp. antárticos en la biodegradación de polietileno PET |
| title_sort | Evaluación de Bacillus sp. antárticos en la biodegradación de polietileno PET |
| topic | Polietileno tereftalato Bacillus sp. Biodegradación Estructura polimérica |
| url | http://repositorio.espam.edu.ec/handle/42000/2084 |