Consorcio microbiano autóctono in vitro en remediación de efluentes en cultivo de camarón (Litopenaeus Vannamei) de agua dulce

The present research work was carried out in the Environmental Chemistry laboratories of the Agroindustrial area and Microbiology of the Agricultural area of the Higher Polytechnic Agricultural School of Manabí Manuel Félix López, located in Calceta - Manabí - Ecuador and its main objective was to e...

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Autor principal: Rodríguez Solórzano, Anthony Miguel (author)
Formato: bachelorThesis
Idioma:spa
Publicado em: 2020
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Acesso em linha:http://repositorio.espam.edu.ec/handle/42000/1331
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Resumo:The present research work was carried out in the Environmental Chemistry laboratories of the Agroindustrial area and Microbiology of the Agricultural area of the Higher Polytechnic Agricultural School of Manabí Manuel Félix López, located in Calceta - Manabí - Ecuador and its main objective was to evaluate the percentage of efficiency of autochthonous microbial consortiums (in vitro) in a shrimp farm located in the Cabello sector, analyzing parameters such as: phosphates, ammonia, nitrites, nitrates, suspended solids, dissolved oxygen, temperature, pH, BOD5 and total coliforms. A Completely Randomized Design with a factor and 4 levels was used, 4 treatments were performed: T1 = called bacterial consortium with a dose of 1,5 ml/dm3 consisting of: Bacillus subtilis (7,8 * 107 CFU/ml) Saccharomices cerevisiae (4,32 * 108 CFU / ml), Lactobacillus plantarum (10,6 * 10⁸ CFU/ml) Lactobacillus acidophilus (1,848 ∗ 109 CFU / ml); T2 = called microalgal consortium with a dose of 1.5 ml / dm3 composed of: Chlamydomonas sp (4,59 ∗ 105 cel/ml), Chlorella sp (3,73 ∗ 106 cel / ml) and Scenedesmus sp (6,2 ∗ 105 cel/ml); T3 = called fungal consortium consisting of T2 treatment plus Trichoderma harzianum fungus (1 * 108 cel/ml). The application of autochthonous microbial consortiums allowed the analyzed parameters to be adjusted to water quality criteria for agricultural, livestock and fresh water discharge use. Of the three treatments, the one with the highest incidence was T2. Finally, the treatments applied statistically show significance of p <0.05 in phosphate, ammonia and total coliforms, while in the other parameters they did not show significance.