Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins
Listeria monocytogenes is a human opportunistic foodborne pathogen that produces life-threatening infections with a high mortality rate. The control of Listeria in the food production environment and effective clinical management of human listeriosis are challenging due to the emergence of antibioti...
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2024
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Accesso online: | https://doi.org/10.1016/j.biochi.2024.05.022 http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/801 |
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author | Bermúdez Puga, Sebastián |
author2 | Dias, Meriellen Lima Reis, Iara Freire de Oliveira, Taciana Yokomizo de Almeida, Sonia Regina Mendes, Maria Anita Moore, Simon J. de Almeida, José R. Proaño Bolaños, Carolina de Souza Oliveira, Ricardo Pinheiro |
author2_role | author author author author author author author author author |
author_facet | Bermúdez Puga, Sebastián Dias, Meriellen Lima Reis, Iara Freire de Oliveira, Taciana Yokomizo de Almeida, Sonia Regina Mendes, Maria Anita Moore, Simon J. de Almeida, José R. Proaño Bolaños, Carolina de Souza Oliveira, Ricardo Pinheiro |
author_role | author |
collection | Repositorio Universidad Regional Amazónica |
dc.creator.none.fl_str_mv | Bermúdez Puga, Sebastián Dias, Meriellen Lima Reis, Iara Freire de Oliveira, Taciana Yokomizo de Almeida, Sonia Regina Mendes, Maria Anita Moore, Simon J. de Almeida, José R. Proaño Bolaños, Carolina de Souza Oliveira, Ricardo Pinheiro |
dc.date.none.fl_str_mv | 2024-11-06T17:15:03Z 2024-11-06T17:15:03Z 2024 |
dc.format.none.fl_str_mv | application/pdf |
dc.identifier.none.fl_str_mv | Bermúdez-Puga, S., Dias, M., Lima Reis, I., Freire de Oliveira, T., Yokomizo de Almeida, S. R., Mendes, M. A., Moore, S. J., Almeida, J. R., Proaño-Bolaños, C., & Pinheiro de Souza Oliveira, R. (2024). Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins. Biochimie, 225, 168-175. https://doi.org/10.1016/j.biochi.2024.05.022 1638-6183 https://doi.org/10.1016/j.biochi.2024.05.022 http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/801 |
dc.language.none.fl_str_mv | en |
dc.publisher.none.fl_str_mv | Scopus |
dc.relation.none.fl_str_mv | PRODUCCIÓN CIENTÍFICA-ARTÍCULO CIENTÍFICO;A-IKIAM-000533 |
dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
dc.source.none.fl_str_mv | reponame:Repositorio Universidad Regional Amazónica instname:Universidad Regional Amazónica instacron:IKIAM |
dc.subject.none.fl_str_mv | Antibacterial Listeria monocytogenes Membrane lysis Metabolomics Skin secretion-derived peptides |
dc.title.none.fl_str_mv | Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins |
dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
description | Listeria monocytogenes is a human opportunistic foodborne pathogen that produces life-threatening infections with a high mortality rate. The control of Listeria in the food production environment and effective clinical management of human listeriosis are challenging due to the emergence of antibiotic resistance. Hence we evaluate the in vitro anti-Listeria activity of two synthetic cruzioseptins reproducing their natural sequences CZS-9, and CZS-12, and one engineered sequence based on CZS-1, named [K4K15]CZS-1. The assessment of the in vitro potential of cruzioseptins, highlighted the promising antibacterial effect of [K4K15]CZS-1 in very low concentrations (0.91 μM) and its thermal stability at high-temperature conditions, is compatible with the food industry. Microscopic and metabolomic analyses suggest cruzioseptin induces anti-Listeria bioactivity through membrane disruption and changes in the intracellular metabolome. We also report that [K4K15]CZS-1 is not resistant to peptidases/proteases emphasizing a key advantage for their use as a food preservative. However, there is a need for further structural and functional optimisations for the potential clinical application as an antibiotic. In conclusion, [K4K15]CZS-1 stand out as membrane-active peptides with the ability to induce shifts in the bacteria metabolome and inspire the development of strategies for the prevention of L. monocytogenes emergence and dissemination. |
eu_rights_str_mv | openAccess |
format | article |
id | IKIAM_6a939de98b165674d9d51e32abec9c20 |
identifier_str_mv | Bermúdez-Puga, S., Dias, M., Lima Reis, I., Freire de Oliveira, T., Yokomizo de Almeida, S. R., Mendes, M. A., Moore, S. J., Almeida, J. R., Proaño-Bolaños, C., & Pinheiro de Souza Oliveira, R. (2024). Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins. Biochimie, 225, 168-175. https://doi.org/10.1016/j.biochi.2024.05.022 1638-6183 |
instacron_str | IKIAM |
institution | IKIAM |
instname_str | Universidad Regional Amazónica |
language_invalid_str_mv | en |
network_acronym_str | IKIAM |
network_name_str | Repositorio Universidad Regional Amazónica |
oai_identifier_str | oai:repositorio.ikiam.edu.ec:RD_IKIAM/801 |
publishDate | 2024 |
publisher.none.fl_str_mv | Scopus |
reponame_str | Repositorio Universidad Regional Amazónica |
repository.mail.fl_str_mv | . |
repository.name.fl_str_mv | Repositorio Universidad Regional Amazónica - Universidad Regional Amazónica |
repository_id_str | 0 |
spelling | Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptinsBermúdez Puga, SebastiánDias, MeriellenLima Reis, IaraFreire de Oliveira, TacianaYokomizo de Almeida, Sonia ReginaMendes, Maria AnitaMoore, Simon J.de Almeida, José R.Proaño Bolaños, Carolinade Souza Oliveira, Ricardo PinheiroAntibacterialListeria monocytogenesMembrane lysisMetabolomicsSkin secretion-derived peptidesListeria monocytogenes is a human opportunistic foodborne pathogen that produces life-threatening infections with a high mortality rate. The control of Listeria in the food production environment and effective clinical management of human listeriosis are challenging due to the emergence of antibiotic resistance. Hence we evaluate the in vitro anti-Listeria activity of two synthetic cruzioseptins reproducing their natural sequences CZS-9, and CZS-12, and one engineered sequence based on CZS-1, named [K4K15]CZS-1. The assessment of the in vitro potential of cruzioseptins, highlighted the promising antibacterial effect of [K4K15]CZS-1 in very low concentrations (0.91 μM) and its thermal stability at high-temperature conditions, is compatible with the food industry. Microscopic and metabolomic analyses suggest cruzioseptin induces anti-Listeria bioactivity through membrane disruption and changes in the intracellular metabolome. We also report that [K4K15]CZS-1 is not resistant to peptidases/proteases emphasizing a key advantage for their use as a food preservative. However, there is a need for further structural and functional optimisations for the potential clinical application as an antibiotic. In conclusion, [K4K15]CZS-1 stand out as membrane-active peptides with the ability to induce shifts in the bacteria metabolome and inspire the development of strategies for the prevention of L. monocytogenes emergence and dissemination.Scopus2024-11-06T17:15:03Z2024-11-06T17:15:03Z2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBermúdez-Puga, S., Dias, M., Lima Reis, I., Freire de Oliveira, T., Yokomizo de Almeida, S. R., Mendes, M. A., Moore, S. J., Almeida, J. R., Proaño-Bolaños, C., & Pinheiro de Souza Oliveira, R. (2024). Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins. Biochimie, 225, 168-175. https://doi.org/10.1016/j.biochi.2024.05.0221638-6183https://doi.org/10.1016/j.biochi.2024.05.022http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/801enPRODUCCIÓN CIENTÍFICA-ARTÍCULO CIENTÍFICO;A-IKIAM-000533info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Regional Amazónicainstname:Universidad Regional Amazónicainstacron:IKIAM2024-11-07T08:00:36Zoai:repositorio.ikiam.edu.ec:RD_IKIAM/801Institucionalhttps://repositorio.ikiam.edu.ec/Universidad públicahttps://www.ikiam.edu.ec/https://repositorio.ikiam.edu.ec/oaiEcuador...opendoar:02024-11-07T08:00:36falseInstitucionalhttps://repositorio.ikiam.edu.ec/Universidad públicahttps://www.ikiam.edu.ec/https://repositorio.ikiam.edu.ec/oai.Ecuador...opendoar:02024-11-07T08:00:36Repositorio Universidad Regional Amazónica - Universidad Regional Amazónicafalse |
spellingShingle | Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins Bermúdez Puga, Sebastián Antibacterial Listeria monocytogenes Membrane lysis Metabolomics Skin secretion-derived peptides |
status_str | publishedVersion |
title | Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins |
title_full | Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins |
title_fullStr | Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins |
title_full_unstemmed | Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins |
title_short | Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins |
title_sort | Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins |
topic | Antibacterial Listeria monocytogenes Membrane lysis Metabolomics Skin secretion-derived peptides |
url | https://doi.org/10.1016/j.biochi.2024.05.022 http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/801 |