Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins

Listeria monocytogenes is a human opportunistic foodborne pathogen that produces life-threatening infections with a high mortality rate. The control of Listeria in the food production environment and effective clinical management of human listeriosis are challenging due to the emergence of antibioti...

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Autore principale: Bermúdez Puga, Sebastián (author)
Altri autori: Dias, Meriellen (author), Lima Reis, Iara (author), Freire de Oliveira, Taciana (author), Yokomizo de Almeida, Sonia Regina (author), Mendes, Maria Anita (author), Moore, Simon J. (author), de Almeida, José R. (author), Proaño Bolaños, Carolina (author), de Souza Oliveira, Ricardo Pinheiro (author)
Natura: article
Pubblicazione: 2024
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Accesso online:https://doi.org/10.1016/j.biochi.2024.05.022
http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/801
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_version_ 1839410476368265216
author Bermúdez Puga, Sebastián
author2 Dias, Meriellen
Lima Reis, Iara
Freire de Oliveira, Taciana
Yokomizo de Almeida, Sonia Regina
Mendes, Maria Anita
Moore, Simon J.
de Almeida, José R.
Proaño Bolaños, Carolina
de Souza Oliveira, Ricardo Pinheiro
author2_role author
author
author
author
author
author
author
author
author
author_facet Bermúdez Puga, Sebastián
Dias, Meriellen
Lima Reis, Iara
Freire de Oliveira, Taciana
Yokomizo de Almeida, Sonia Regina
Mendes, Maria Anita
Moore, Simon J.
de Almeida, José R.
Proaño Bolaños, Carolina
de Souza Oliveira, Ricardo Pinheiro
author_role author
collection Repositorio Universidad Regional Amazónica
dc.creator.none.fl_str_mv Bermúdez Puga, Sebastián
Dias, Meriellen
Lima Reis, Iara
Freire de Oliveira, Taciana
Yokomizo de Almeida, Sonia Regina
Mendes, Maria Anita
Moore, Simon J.
de Almeida, José R.
Proaño Bolaños, Carolina
de Souza Oliveira, Ricardo Pinheiro
dc.date.none.fl_str_mv 2024-11-06T17:15:03Z
2024-11-06T17:15:03Z
2024
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv Bermúdez-Puga, S., Dias, M., Lima Reis, I., Freire de Oliveira, T., Yokomizo de Almeida, S. R., Mendes, M. A., Moore, S. J., Almeida, J. R., Proaño-Bolaños, C., & Pinheiro de Souza Oliveira, R. (2024). Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins. Biochimie, 225, 168-175. https://doi.org/10.1016/j.biochi.2024.05.022
1638-6183
https://doi.org/10.1016/j.biochi.2024.05.022
http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/801
dc.language.none.fl_str_mv en
dc.publisher.none.fl_str_mv Scopus
dc.relation.none.fl_str_mv PRODUCCIÓN CIENTÍFICA-ARTÍCULO CIENTÍFICO;A-IKIAM-000533
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.source.none.fl_str_mv reponame:Repositorio Universidad Regional Amazónica
instname:Universidad Regional Amazónica
instacron:IKIAM
dc.subject.none.fl_str_mv Antibacterial
Listeria monocytogenes
Membrane lysis
Metabolomics
Skin secretion-derived peptides
dc.title.none.fl_str_mv Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
description Listeria monocytogenes is a human opportunistic foodborne pathogen that produces life-threatening infections with a high mortality rate. The control of Listeria in the food production environment and effective clinical management of human listeriosis are challenging due to the emergence of antibiotic resistance. Hence we evaluate the in vitro anti-Listeria activity of two synthetic cruzioseptins reproducing their natural sequences CZS-9, and CZS-12, and one engineered sequence based on CZS-1, named [K4K15]CZS-1. The assessment of the in vitro potential of cruzioseptins, highlighted the promising antibacterial effect of [K4K15]CZS-1 in very low concentrations (0.91 μM) and its thermal stability at high-temperature conditions, is compatible with the food industry. Microscopic and metabolomic analyses suggest cruzioseptin induces anti-Listeria bioactivity through membrane disruption and changes in the intracellular metabolome. We also report that [K4K15]CZS-1 is not resistant to peptidases/proteases emphasizing a key advantage for their use as a food preservative. However, there is a need for further structural and functional optimisations for the potential clinical application as an antibiotic. In conclusion, [K4K15]CZS-1 stand out as membrane-active peptides with the ability to induce shifts in the bacteria metabolome and inspire the development of strategies for the prevention of L. monocytogenes emergence and dissemination.
eu_rights_str_mv openAccess
format article
id IKIAM_6a939de98b165674d9d51e32abec9c20
identifier_str_mv Bermúdez-Puga, S., Dias, M., Lima Reis, I., Freire de Oliveira, T., Yokomizo de Almeida, S. R., Mendes, M. A., Moore, S. J., Almeida, J. R., Proaño-Bolaños, C., & Pinheiro de Souza Oliveira, R. (2024). Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins. Biochimie, 225, 168-175. https://doi.org/10.1016/j.biochi.2024.05.022
1638-6183
instacron_str IKIAM
institution IKIAM
instname_str Universidad Regional Amazónica
language_invalid_str_mv en
network_acronym_str IKIAM
network_name_str Repositorio Universidad Regional Amazónica
oai_identifier_str oai:repositorio.ikiam.edu.ec:RD_IKIAM/801
publishDate 2024
publisher.none.fl_str_mv Scopus
reponame_str Repositorio Universidad Regional Amazónica
repository.mail.fl_str_mv .
repository.name.fl_str_mv Repositorio Universidad Regional Amazónica - Universidad Regional Amazónica
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spelling Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptinsBermúdez Puga, SebastiánDias, MeriellenLima Reis, IaraFreire de Oliveira, TacianaYokomizo de Almeida, Sonia ReginaMendes, Maria AnitaMoore, Simon J.de Almeida, José R.Proaño Bolaños, Carolinade Souza Oliveira, Ricardo PinheiroAntibacterialListeria monocytogenesMembrane lysisMetabolomicsSkin secretion-derived peptidesListeria monocytogenes is a human opportunistic foodborne pathogen that produces life-threatening infections with a high mortality rate. The control of Listeria in the food production environment and effective clinical management of human listeriosis are challenging due to the emergence of antibiotic resistance. Hence we evaluate the in vitro anti-Listeria activity of two synthetic cruzioseptins reproducing their natural sequences CZS-9, and CZS-12, and one engineered sequence based on CZS-1, named [K4K15]CZS-1. The assessment of the in vitro potential of cruzioseptins, highlighted the promising antibacterial effect of [K4K15]CZS-1 in very low concentrations (0.91 μM) and its thermal stability at high-temperature conditions, is compatible with the food industry. Microscopic and metabolomic analyses suggest cruzioseptin induces anti-Listeria bioactivity through membrane disruption and changes in the intracellular metabolome. We also report that [K4K15]CZS-1 is not resistant to peptidases/proteases emphasizing a key advantage for their use as a food preservative. However, there is a need for further structural and functional optimisations for the potential clinical application as an antibiotic. In conclusion, [K4K15]CZS-1 stand out as membrane-active peptides with the ability to induce shifts in the bacteria metabolome and inspire the development of strategies for the prevention of L. monocytogenes emergence and dissemination.Scopus2024-11-06T17:15:03Z2024-11-06T17:15:03Z2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBermúdez-Puga, S., Dias, M., Lima Reis, I., Freire de Oliveira, T., Yokomizo de Almeida, S. R., Mendes, M. A., Moore, S. J., Almeida, J. R., Proaño-Bolaños, C., & Pinheiro de Souza Oliveira, R. (2024). Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins. Biochimie, 225, 168-175. https://doi.org/10.1016/j.biochi.2024.05.0221638-6183https://doi.org/10.1016/j.biochi.2024.05.022http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/801enPRODUCCIÓN CIENTÍFICA-ARTÍCULO CIENTÍFICO;A-IKIAM-000533info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Regional Amazónicainstname:Universidad Regional Amazónicainstacron:IKIAM2024-11-07T08:00:36Zoai:repositorio.ikiam.edu.ec:RD_IKIAM/801Institucionalhttps://repositorio.ikiam.edu.ec/Universidad públicahttps://www.ikiam.edu.ec/https://repositorio.ikiam.edu.ec/oaiEcuador...opendoar:02024-11-07T08:00:36falseInstitucionalhttps://repositorio.ikiam.edu.ec/Universidad públicahttps://www.ikiam.edu.ec/https://repositorio.ikiam.edu.ec/oai.Ecuador...opendoar:02024-11-07T08:00:36Repositorio Universidad Regional Amazónica - Universidad Regional Amazónicafalse
spellingShingle Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins
Bermúdez Puga, Sebastián
Antibacterial
Listeria monocytogenes
Membrane lysis
Metabolomics
Skin secretion-derived peptides
status_str publishedVersion
title Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins
title_full Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins
title_fullStr Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins
title_full_unstemmed Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins
title_short Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins
title_sort Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins
topic Antibacterial
Listeria monocytogenes
Membrane lysis
Metabolomics
Skin secretion-derived peptides
url https://doi.org/10.1016/j.biochi.2024.05.022
http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/801