Skin secretion transcriptome remains in chromatographic fractions suitable for molecular cloning

Traditional sources of mRNA for molecular cloning on amphibian skin secretion studies have been the frog's skin and skin secretions. Here, we demonstrate that mRNA isolated from chromatographic fractions of skin secretions is amenable for molecular cloning assays. We identified precursor sequen...

• Hovedforfatter: Proaño Bolaños, Carolina (author)
• Andre forfattere: Zhou, Mei (author), Chen, Tianbao (author), Shaw, Chris (author)
• Format: article
• Udgivet: 2019
• Fag: MRNA; Transcriptome; Liquid chromatography; Molecular cloninG; Skin secretion
• Online adgang: http://repositorio.ikiam.edu.ec:8080/jspui/handle/RD_IKIAM/197; http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/197; https://doi.org/10.1016/j.ab.2018.10.006