Skin secretion transcriptome remains in chromatographic fractions suitable for molecular cloning

Traditional sources of mRNA for molecular cloning on amphibian skin secretion studies have been the frog's skin and skin secretions. Here, we demonstrate that mRNA isolated from chromatographic fractions of skin secretions is amenable for molecular cloning assays. We identified precursor sequen...

• מחבר ראשי: Proaño Bolaños, Carolina (author)
• מחברים אחרים: Zhou, Mei (author), Chen, Tianbao (author), Shaw, Chris (author)
• פורמט: article
• יצא לאור: 2019
• נושאים: MRNA; Transcriptome; Liquid chromatography; Molecular cloninG; Skin secretion
• גישה מקוונת: http://repositorio.ikiam.edu.ec:8080/jspui/handle/RD_IKIAM/197; http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/197; https://doi.org/10.1016/j.ab.2018.10.006