Evaluación de la actividad antifúngica in vitro de extractos totales de dos especies vegetales como Ortiga y Hierba Mora contra el hongo Colletotrichum spp. aislado de tomate de árbol
This research focuses on the exploration of alternative and sustainable methods for the control of pathogens in agriculture. The main objective was to determine the effectiveness of total extracts of nettle (Urtica dioica) and blackberry (Solanum nigrum) in inhibiting the growth of the fungus Collet...
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| 格式: | bachelorThesis |
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| 在線閱讀: | https://repositorio.puce.edu.ec/handle/123456789/44596 |
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| author | Erazo Pozo, Andrea Estefania |
| author_facet | Erazo Pozo, Andrea Estefania |
| author_role | author |
| collection | Repositorio Pontificia Universidad Católica del Ecuador |
| dc.contributor.none.fl_str_mv | Ordóñez Vivanco, Yadira Fernanda |
| dc.creator.none.fl_str_mv | Erazo Pozo, Andrea Estefania |
| dc.date.none.fl_str_mv | 2024-10-24T18:39:57Z 2024-10-24T18:39:57Z 2024 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://repositorio.puce.edu.ec/handle/123456789/44596 |
| dc.language.none.fl_str_mv | es |
| dc.publisher.none.fl_str_mv | PUCE - Ibarra |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Pontificia Universidad Católica del Ecuador instname:Pontificia Universidad Católica del Ecuador instacron:PUCE |
| dc.subject.none.fl_str_mv | colletotrichum, inhibición de crecimiento micelar, extractos vegetales, Urtica dioica, Solanum nigrum |
| dc.title.none.fl_str_mv | Evaluación de la actividad antifúngica in vitro de extractos totales de dos especies vegetales como Ortiga y Hierba Mora contra el hongo Colletotrichum spp. aislado de tomate de árbol |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
| description | This research focuses on the exploration of alternative and sustainable methods for the control of pathogens in agriculture. The main objective was to determine the effectiveness of total extracts of nettle (Urtica dioica) and blackberry (Solanum nigrum) in inhibiting the growth of the fungus Colletotrichum tamarilloi, a pathogen responsible for anthracnosis in the tree tomato. In this study, in vitro tests were carried out using serial concentrations of total plant extracts. The treatments were applied on petri dishes inoculated with C. tamarilloi, and mycelial growth was evaluated over a period of seven days. The results showed that the Nettle extract was more effective in inhibiting the growth of C. tamarilloi compared to the extract of Blackberry Grass. In particular, treatment with the highest concentration of Nettle extract (T1: 500 mg/ml) showed an inhibition percentage of 96.21% for day 4, while treatment with Blackberry Herb showed a maximum inhibition percentage of 90.27% under the same conditions. Chemical control (T6) was used as a reference, reaching 100% inhibition, which made it possible to compare the effectiveness of plant extracts. The statistical analysis showed that the differences between the treatments were highly significant, with p-values less than 0.0001, indicating that the variability observed in the inhibition is not random, but is directly associated with the treatments applied. The findings of this study suggest that both Nettle and Blackberry Grass have a high potential as biofungicides, offering a viable and sustainable alternative to traditional chemical fungicides. The implementation of these extracts could reduce the use of agrochemicals, promoting more sustainable and ecological agricultural practices. |
| eu_rights_str_mv | openAccess |
| format | bachelorThesis |
| id | PUCE_6cb0db6800a6507218755d8a6191840d |
| instacron_str | PUCE |
| institution | PUCE |
| instname_str | Pontificia Universidad Católica del Ecuador |
| language_invalid_str_mv | es |
| network_acronym_str | PUCE |
| network_name_str | Repositorio Pontificia Universidad Católica del Ecuador |
| oai_identifier_str | oai:repositorio.puce.edu.ec:123456789/44596 |
| publishDate | 2024 |
| publisher.none.fl_str_mv | PUCE - Ibarra |
| reponame_str | Repositorio Pontificia Universidad Católica del Ecuador |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Repositorio Pontificia Universidad Católica del Ecuador - Pontificia Universidad Católica del Ecuador |
| repository_id_str | 2180 |
| spelling | Evaluación de la actividad antifúngica in vitro de extractos totales de dos especies vegetales como Ortiga y Hierba Mora contra el hongo Colletotrichum spp. aislado de tomate de árbolErazo Pozo, Andrea Estefaniacolletotrichum, inhibición de crecimiento micelar, extractos vegetales, Urtica dioica, Solanum nigrumThis research focuses on the exploration of alternative and sustainable methods for the control of pathogens in agriculture. The main objective was to determine the effectiveness of total extracts of nettle (Urtica dioica) and blackberry (Solanum nigrum) in inhibiting the growth of the fungus Colletotrichum tamarilloi, a pathogen responsible for anthracnosis in the tree tomato. In this study, in vitro tests were carried out using serial concentrations of total plant extracts. The treatments were applied on petri dishes inoculated with C. tamarilloi, and mycelial growth was evaluated over a period of seven days. The results showed that the Nettle extract was more effective in inhibiting the growth of C. tamarilloi compared to the extract of Blackberry Grass. In particular, treatment with the highest concentration of Nettle extract (T1: 500 mg/ml) showed an inhibition percentage of 96.21% for day 4, while treatment with Blackberry Herb showed a maximum inhibition percentage of 90.27% under the same conditions. Chemical control (T6) was used as a reference, reaching 100% inhibition, which made it possible to compare the effectiveness of plant extracts. The statistical analysis showed that the differences between the treatments were highly significant, with p-values less than 0.0001, indicating that the variability observed in the inhibition is not random, but is directly associated with the treatments applied. The findings of this study suggest that both Nettle and Blackberry Grass have a high potential as biofungicides, offering a viable and sustainable alternative to traditional chemical fungicides. The implementation of these extracts could reduce the use of agrochemicals, promoting more sustainable and ecological agricultural practices.La presente investigación se focaliza en la exploración de métodos alternativos y sostenibles para el control de patógenos en la agricultura. El objetivo principal fue determinar la eficacia de los extractos totales de ortiga (Urtica dioica) y hierba mora (Solanum nigrum) en la inhibición del crecimiento del hongo Colletotrichum tamarilloi, un agente patógeno responsable de la antracnosis en el tomate de árbol. En este estudio se llevaron a cabo ensayos in vitro utilizando concentraciones seriadas de extractos totales vegetales. Los tratamientos se aplicaron en placas de Petri inoculadas con C. tamarilloi, y se evaluó el crecimiento micelial a lo largo de un período de siete días. Los resultados mostraron que el extracto de Ortiga fue más efectivo en la inhibición del crecimiento de C. tamarilloi en comparación con el extracto de Hierba Mora. En particular, el tratamiento con la mayor concentración de extracto de Ortiga (T1: 500 mg/ml) mostró un porcentaje de inhibición del 96.21% para el día 4, mientras que el tratamiento con Hierba Mora presentó un porcentaje máximo de inhibición del 90.27% en las mismas condiciones. El control químico (T6) se utilizó como referencia, alcanzando un 100% de inhibición, lo que permitió comparar la eficacia de los extractos vegetales. El análisis estadístico demostró que las diferencias entre los tratamientos fueron altamente significativas, con p-valores menores a 0.0001, lo que indica que la variabilidad observada en la inhibición no es aleatoria, sino que está directamente asociada con los tratamientos aplicados. Los hallazgos de este estudio sugieren que tanto la Ortiga como la Hierba Mora tienen un alto potencial como biofungicidas, ofreciendo una alternativa viable y sostenible a los fungicidas químicos tradicionales. La implementación de estos extractos podría reducir el uso de agroquímicos, promoviendo prácticas agrícolas más sostenibles y ecológicas.This research focuses on the exploration of alternative and sustainable methods for the control of pathogens in agriculture. The main objective was to determine the effectiveness of total extracts of nettle (Urtica dioica) and blackberry (Solanum nigrum) in inhibiting the growth of the fungus Colletotrichum tamarilloi, a pathogen responsible for anthracnosis in the tree tomato. In this study, in vitro tests were carried out using serial concentrations of total plant extracts. The treatments were applied on petri dishes inoculated with C. tamarilloi, and mycelial growth was evaluated over a period of seven days. The results showed that the Nettle extract was more effective in inhibiting the growth of C. tamarilloi compared to the extract of Blackberry Grass. In particular, treatment with the highest concentration of Nettle extract (T1: 500 mg/ml) showed an inhibition percentage of 96.21% for day 4, while treatment with Blackberry Herb showed a maximum inhibition percentage of 90.27% under the same conditions. Chemical control (T6) was used as a reference, reaching 100% inhibition, which made it possible to compare the effectiveness of plant extracts. The statistical analysis showed that the differences between the treatments were highly significant, with p-values less than 0.0001, indicating that the variability observed in the inhibition is not random, but is directly associated with the treatments applied. The findings of this study suggest that both Nettle and Blackberry Grass have a high potential as biofungicides, offering a viable and sustainable alternative to traditional chemical fungicides. The implementation of these extracts could reduce the use of agrochemicals, promoting more sustainable and ecological agricultural practices.PUCE - IbarraOrdóñez Vivanco, Yadira Fernanda2024-10-24T18:39:57Z2024-10-24T18:39:57Z2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfhttps://repositorio.puce.edu.ec/handle/123456789/44596esinfo:eu-repo/semantics/openAccessreponame:Repositorio Pontificia Universidad Católica del Ecuadorinstname:Pontificia Universidad Católica del Ecuadorinstacron:PUCE2025-06-09T21:16:08Zoai:repositorio.puce.edu.ec:123456789/44596Institucionalhttp://repositorio.puce.edu.ec/Institución privadahttps://www.puce.edu.ec/http://repositorio.puce.edu.ec/oai.Ecuador...opendoar:21802025-07-05T15:27:35.801502Repositorio Pontificia Universidad Católica del Ecuador - Pontificia Universidad Católica del Ecuadortrue |
| spellingShingle | Evaluación de la actividad antifúngica in vitro de extractos totales de dos especies vegetales como Ortiga y Hierba Mora contra el hongo Colletotrichum spp. aislado de tomate de árbol Erazo Pozo, Andrea Estefania colletotrichum, inhibición de crecimiento micelar, extractos vegetales, Urtica dioica, Solanum nigrum |
| status_str | publishedVersion |
| title | Evaluación de la actividad antifúngica in vitro de extractos totales de dos especies vegetales como Ortiga y Hierba Mora contra el hongo Colletotrichum spp. aislado de tomate de árbol |
| title_full | Evaluación de la actividad antifúngica in vitro de extractos totales de dos especies vegetales como Ortiga y Hierba Mora contra el hongo Colletotrichum spp. aislado de tomate de árbol |
| title_fullStr | Evaluación de la actividad antifúngica in vitro de extractos totales de dos especies vegetales como Ortiga y Hierba Mora contra el hongo Colletotrichum spp. aislado de tomate de árbol |
| title_full_unstemmed | Evaluación de la actividad antifúngica in vitro de extractos totales de dos especies vegetales como Ortiga y Hierba Mora contra el hongo Colletotrichum spp. aislado de tomate de árbol |
| title_short | Evaluación de la actividad antifúngica in vitro de extractos totales de dos especies vegetales como Ortiga y Hierba Mora contra el hongo Colletotrichum spp. aislado de tomate de árbol |
| title_sort | Evaluación de la actividad antifúngica in vitro de extractos totales de dos especies vegetales como Ortiga y Hierba Mora contra el hongo Colletotrichum spp. aislado de tomate de árbol |
| topic | colletotrichum, inhibición de crecimiento micelar, extractos vegetales, Urtica dioica, Solanum nigrum |
| url | https://repositorio.puce.edu.ec/handle/123456789/44596 |