Evaluación in vitro del hongo Trichoderma spp. como potencial antagonista en el control de la enfermedad antracnosis ocasionada por el fitopatógeno Colletotrichum gloeosporioides Penz, en granadilla Passiflora ligularis
Globally, the foods that are produced are increasingly harmful to human health due to the high content of chemical residues they contain. One of the main problems is the excessive and inappropriate use of agricultural inputs to control fungal diseases in passion fruit cultivation Passiflora ligulari...
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author | Fuertes Chamorro, Anderson Alexis |
author_facet | Fuertes Chamorro, Anderson Alexis |
author_role | author |
collection | Repositorio Pontificia Universidad Católica del Ecuador |
dc.contributor.none.fl_str_mv | Mafla Andrade, Santiago Xavier |
dc.creator.none.fl_str_mv | Fuertes Chamorro, Anderson Alexis |
dc.date.none.fl_str_mv | 2022 2024-04-08T17:29:17Z 2024-04-08T17:29:17Z |
dc.format.none.fl_str_mv | application/pdf |
dc.identifier.none.fl_str_mv | T/635.21/F954e/2022 https://repositorio.puce.edu.ec/handle/123456789/42673 |
dc.language.none.fl_str_mv | es |
dc.publisher.none.fl_str_mv | PUCE - Ibarra |
dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
dc.source.none.fl_str_mv | reponame:Repositorio Pontificia Universidad Católica del Ecuador instname:Pontificia Universidad Católica del Ecuador instacron:PUCE |
dc.subject.none.fl_str_mv | trichoderma, passiflora ligularis, control biológico, control químico, colletotrichum gloeosporioides |
dc.title.none.fl_str_mv | Evaluación in vitro del hongo Trichoderma spp. como potencial antagonista en el control de la enfermedad antracnosis ocasionada por el fitopatógeno Colletotrichum gloeosporioides Penz, en granadilla Passiflora ligularis |
dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
description | Globally, the foods that are produced are increasingly harmful to human health due to the high content of chemical residues they contain. One of the main problems is the excessive and inappropriate use of agricultural inputs to control fungal diseases in passion fruit cultivation Passiflora ligularis P., in addition to low knowledge on integrated management of pests and diseases by producers. This is why one of the alternatives to reduce the use of chemical fungicides such as Mancozeb, for anthracnose management, is biological control that reduces chemical residues in the fruit. Trichoderma spp is an excellent biological controller of some pathogens, such as Fusarium oxysporum, Botrytis cinerea, Phytophthora infestans, which cause production losses and decrease yields. The main objective of this study was to evaluate in vitro the fungus Trichoderma spp as a potential antagonist in the control of anthracnose disease caused by the phytopathogen Colletotrichum gloeosporioides Penz in granadilla. The research was carried out in the laboratories of PUCE-SI, Ibarra canton, Imbabura province, and a completely randomized design was used with three doses of Trichoderma spp (1x103 , 1x105 , 1x107 spores/ml) and a control based on Mancozeb with three repetitions in each treatment. The dual culture technique was used, where the best control of the pathogen was obtained with a dose of 1x107 ufc/ml. In addition, the two best doses were evaluated in humid chambers with infected leaves, measuring the diametral growth of the lesion, obtaining the best result with 1x107 spores/ml of Trichoderma spp. For this reason, the antagonist under study is an alternative to control the anthracnose disease in passion fruit, contributing to the healthy production of agricultural products and the care of the environment. |
eu_rights_str_mv | openAccess |
format | bachelorThesis |
id | PUCE_dd1b51a1e87884ef8a1232f37463a9b6 |
identifier_str_mv | T/635.21/F954e/2022 |
instacron_str | PUCE |
institution | PUCE |
instname_str | Pontificia Universidad Católica del Ecuador |
language_invalid_str_mv | es |
network_acronym_str | PUCE |
network_name_str | Repositorio Pontificia Universidad Católica del Ecuador |
oai_identifier_str | oai:repositorio.puce.edu.ec:123456789/42673 |
publishDate | 2022 |
publisher.none.fl_str_mv | PUCE - Ibarra |
reponame_str | Repositorio Pontificia Universidad Católica del Ecuador |
repository.mail.fl_str_mv | . |
repository.name.fl_str_mv | Repositorio Pontificia Universidad Católica del Ecuador - Pontificia Universidad Católica del Ecuador |
repository_id_str | 2180 |
spelling | Evaluación in vitro del hongo Trichoderma spp. como potencial antagonista en el control de la enfermedad antracnosis ocasionada por el fitopatógeno Colletotrichum gloeosporioides Penz, en granadilla Passiflora ligularisFuertes Chamorro, Anderson Alexistrichoderma, passiflora ligularis, control biológico, control químico, colletotrichum gloeosporioidesGlobally, the foods that are produced are increasingly harmful to human health due to the high content of chemical residues they contain. One of the main problems is the excessive and inappropriate use of agricultural inputs to control fungal diseases in passion fruit cultivation Passiflora ligularis P., in addition to low knowledge on integrated management of pests and diseases by producers. This is why one of the alternatives to reduce the use of chemical fungicides such as Mancozeb, for anthracnose management, is biological control that reduces chemical residues in the fruit. Trichoderma spp is an excellent biological controller of some pathogens, such as Fusarium oxysporum, Botrytis cinerea, Phytophthora infestans, which cause production losses and decrease yields. The main objective of this study was to evaluate in vitro the fungus Trichoderma spp as a potential antagonist in the control of anthracnose disease caused by the phytopathogen Colletotrichum gloeosporioides Penz in granadilla. The research was carried out in the laboratories of PUCE-SI, Ibarra canton, Imbabura province, and a completely randomized design was used with three doses of Trichoderma spp (1x103 , 1x105 , 1x107 spores/ml) and a control based on Mancozeb with three repetitions in each treatment. The dual culture technique was used, where the best control of the pathogen was obtained with a dose of 1x107 ufc/ml. In addition, the two best doses were evaluated in humid chambers with infected leaves, measuring the diametral growth of the lesion, obtaining the best result with 1x107 spores/ml of Trichoderma spp. For this reason, the antagonist under study is an alternative to control the anthracnose disease in passion fruit, contributing to the healthy production of agricultural products and the care of the environment.A nivel mundial los alimentos que se producen son cada vez más perjudiciales para la salud humana debido al alto contenido de residuos químicos que contienen. Uno de los principales problemas es el uso excesivo e inadecuado de insumos agrícolas para controlar enfermedades fúngicas en el cultivo de granadilla Passiflora ligularis J. Además de un bajo conocimiento sobre manejo integrado de plagas por parte de los productores. Es por esto que una de las alternativas para reducir el uso de fungicidas como Mancozeb, para manejo de antracnosis, es el control biológico que disminuye los residuos químicos en la fruta. Trichoderma spp. es un excelente controlador biológico de algunos patógenos, como Fusarium oxysporum, Botrytis cinérea, Phytophthora infestans, que ocasionan pérdidas en las producciones y disminuyen los rendimientos. El principal objetivo del presente estudio fue evaluar in vitro el hongo Trichoderma spp. como potencial antagonista en el control de la enfermedad antracnosis ocasionada por el fitopatógeno Colletotrichum gloeosporioides Penz en granadilla. La investigación se realizó en los laboratorios de la PUCE-SI, cantón Ibarra, provincia de Imbabura y se utilizó un diseño completamente al azar con tres dosis de Trichoderma spp (1x103 , 1x105 , 1x107 ufc/ml) y un testigo a base de Mancozeb con tres repeticiones. Se utilizó la técnica de cultivos duales en donde se obtuvo el mejor control del patógeno con la dosis de 1x107 ufc/ml. Además, las dos mejores dosis se evaluaron en cámaras húmedas con hojas infectadas midiendo el crecimiento diametral de la lesión obteniendo el mejor resultado con 1x107 ufc/ml de Trichoderma spp. Por esta razón el antagonista en estudio resulta una alternativa de control a la enfermedad antracnosis en granadilla aportando a la producción sana de productos agrícolas y al cuidado del medio ambiente.Globally, the foods that are produced are increasingly harmful to human health due to the high content of chemical residues they contain. One of the main problems is the excessive and inappropriate use of agricultural inputs to control fungal diseases in passion fruit cultivation Passiflora ligularis P., in addition to low knowledge on integrated management of pests and diseases by producers. This is why one of the alternatives to reduce the use of chemical fungicides such as Mancozeb, for anthracnose management, is biological control that reduces chemical residues in the fruit. Trichoderma spp is an excellent biological controller of some pathogens, such as Fusarium oxysporum, Botrytis cinerea, Phytophthora infestans, which cause production losses and decrease yields. The main objective of this study was to evaluate in vitro the fungus Trichoderma spp as a potential antagonist in the control of anthracnose disease caused by the phytopathogen Colletotrichum gloeosporioides Penz in granadilla. The research was carried out in the laboratories of PUCE-SI, Ibarra canton, Imbabura province, and a completely randomized design was used with three doses of Trichoderma spp (1x103 , 1x105 , 1x107 spores/ml) and a control based on Mancozeb with three repetitions in each treatment. The dual culture technique was used, where the best control of the pathogen was obtained with a dose of 1x107 ufc/ml. In addition, the two best doses were evaluated in humid chambers with infected leaves, measuring the diametral growth of the lesion, obtaining the best result with 1x107 spores/ml of Trichoderma spp. For this reason, the antagonist under study is an alternative to control the anthracnose disease in passion fruit, contributing to the healthy production of agricultural products and the care of the environment.PUCE - IbarraMafla Andrade, Santiago Xavier2024-04-08T17:29:17Z2024-04-08T17:29:17Z2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfT/635.21/F954e/2022https://repositorio.puce.edu.ec/handle/123456789/42673esinfo:eu-repo/semantics/openAccessreponame:Repositorio Pontificia Universidad Católica del Ecuadorinstname:Pontificia Universidad Católica del Ecuadorinstacron:PUCE2025-06-09T21:10:10Zoai:repositorio.puce.edu.ec:123456789/42673Institucionalhttp://repositorio.puce.edu.ec/Institución privadahttps://www.puce.edu.ec/http://repositorio.puce.edu.ec/oai.Ecuador...opendoar:21802025-07-05T15:18:32.735620Repositorio Pontificia Universidad Católica del Ecuador - Pontificia Universidad Católica del Ecuadortrue |
spellingShingle | Evaluación in vitro del hongo Trichoderma spp. como potencial antagonista en el control de la enfermedad antracnosis ocasionada por el fitopatógeno Colletotrichum gloeosporioides Penz, en granadilla Passiflora ligularis Fuertes Chamorro, Anderson Alexis trichoderma, passiflora ligularis, control biológico, control químico, colletotrichum gloeosporioides |
status_str | publishedVersion |
title | Evaluación in vitro del hongo Trichoderma spp. como potencial antagonista en el control de la enfermedad antracnosis ocasionada por el fitopatógeno Colletotrichum gloeosporioides Penz, en granadilla Passiflora ligularis |
title_full | Evaluación in vitro del hongo Trichoderma spp. como potencial antagonista en el control de la enfermedad antracnosis ocasionada por el fitopatógeno Colletotrichum gloeosporioides Penz, en granadilla Passiflora ligularis |
title_fullStr | Evaluación in vitro del hongo Trichoderma spp. como potencial antagonista en el control de la enfermedad antracnosis ocasionada por el fitopatógeno Colletotrichum gloeosporioides Penz, en granadilla Passiflora ligularis |
title_full_unstemmed | Evaluación in vitro del hongo Trichoderma spp. como potencial antagonista en el control de la enfermedad antracnosis ocasionada por el fitopatógeno Colletotrichum gloeosporioides Penz, en granadilla Passiflora ligularis |
title_short | Evaluación in vitro del hongo Trichoderma spp. como potencial antagonista en el control de la enfermedad antracnosis ocasionada por el fitopatógeno Colletotrichum gloeosporioides Penz, en granadilla Passiflora ligularis |
title_sort | Evaluación in vitro del hongo Trichoderma spp. como potencial antagonista en el control de la enfermedad antracnosis ocasionada por el fitopatógeno Colletotrichum gloeosporioides Penz, en granadilla Passiflora ligularis |
topic | trichoderma, passiflora ligularis, control biológico, control químico, colletotrichum gloeosporioides |
url | https://repositorio.puce.edu.ec/handle/123456789/42673 |