Thyme (Thymus vulgaris) as an antimicrobial agent in the production of fresh cheese

The effect of the use of the thyme Thymus vulgaris leaves in the development of. Staphylococcus aureus, a microorganism that is associated with outbreaks of food poisoning and which is commonly present in fresh cheese of the artisanal type, for this purpose, cheese was prepared by inoculating strain...

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Autore principale: Mejía López, Ana (author)
Altri autori: Herrera, Byron (author), Salazar, Mario (author), Rojas Vallejo, María Fernanda (author), Gavín, Verónica (author), Escobar Machado, José Antonio (author)
Natura: article
Lingua:spa
Pubblicazione: 2017
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Accesso online:https://revistas.uea.edu.ec/index.php/racyt/article/view/71
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author Mejía López, Ana
author2 Herrera, Byron
Salazar, Mario
Rojas Vallejo, María Fernanda
Gavín, Verónica
Escobar Machado, José Antonio
author2_role author
author
author
author
author
author_facet Mejía López, Ana
Herrera, Byron
Salazar, Mario
Rojas Vallejo, María Fernanda
Gavín, Verónica
Escobar Machado, José Antonio
author_role author
collection Revista Amazónica. Ciencia y Tecnología
dc.creator.none.fl_str_mv Mejía López, Ana
Herrera, Byron
Salazar, Mario
Rojas Vallejo, María Fernanda
Gavín, Verónica
Escobar Machado, José Antonio
dc.date.none.fl_str_mv 2017-04-27
dc.format.none.fl_str_mv application/pdf
text/html
dc.identifier.none.fl_str_mv https://revistas.uea.edu.ec/index.php/racyt/article/view/71
10.59410/RACYT-v06n01ep05-0071
dc.language.none.fl_str_mv spa
dc.publisher.none.fl_str_mv Universidad Estatal Amazónica
dc.relation.none.fl_str_mv https://revistas.uea.edu.ec/index.php/racyt/article/view/71/74
https://revistas.uea.edu.ec/index.php/racyt/article/view/71/235
dc.rights.none.fl_str_mv Derechos de autor 2017 Revista Amazónica. Ciencia y Tecnología
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
dc.source.none.fl_str_mv Revista Amazónica. Ciencia y Tecnología; Vol. 6 No. 1 (2017): Revista Amazónica Ciencia y Tecnología ; 45 - 54
Revista Amazónica. Ciencia y Tecnología; Vol. 6 Núm. 1 (2017): Revista Amazónica Ciencia y Tecnología ; 45 - 54
1390-8049
1390-5600
reponame:Revista Amazónica. Ciencia y Tecnología
instname:Universidad Estatal Amazónica
instacron:UEA
dc.subject.none.fl_str_mv Queso
inhibición de Staphylococcus aureus
tomillo
Cheese
inhibition of Staphylococcus aureu
thyme
dc.title.none.fl_str_mv Thyme (Thymus vulgaris) as an antimicrobial agent in the production of fresh cheese
Tomillo (Thymus vulgaris) como agente antimicrobiano en la producción de queso fresco
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
description The effect of the use of the thyme Thymus vulgaris leaves in the development of. Staphylococcus aureus, a microorganism that is associated with outbreaks of food poisoning and which is commonly present in fresh cheese of the artisanal type, for this purpose, cheese was prepared by inoculating strains of S. aureus ATCC 25923 previously suspended with a turbidity equivalent to the scale of 0.5 McFarland, and was evaluated through a completely randomized design with 3 replicates, where the factors under study were 2 types of processes (1) in sheets incorporated in cheese, (2) in infusion in salty water, and 4 Concentrations (0, 1, 075 and 0.5%). Colony-forming units were determined following the official APHA methodology on days 1, 8, 15, and 30 after their production. As a result, a significant reduction of the microbial load in the experimental plates (1 and 075% with thyme) was obtained in comparison to the control plates (0%) in process 1, whereas there was reduction for all the concentrations tested when added the infused thyme during the salting of the cheese. Concluding that at greater concentration of thyme greater inhibitory effect on S. aureus and could be used in the preservation of food as the main natural antimicrobial compounds in order to ensure the production of microbiologically stable food.
eu_rights_str_mv openAccess
format article
id RACYT_0aaa13bdc4eb60ffbdf44ebeb12fe268
identifier_str_mv 10.59410/RACYT-v06n01ep05-0071
instacron_str UEA
institution UEA
instname_str Universidad Estatal Amazónica
language spa
network_acronym_str RACYT
network_name_str Revista Amazónica. Ciencia y Tecnología
oai_identifier_str oai:ojs_revista.www.uea.edu.ec:article/71
publishDate 2017
publisher.none.fl_str_mv Universidad Estatal Amazónica
reponame_str Revista Amazónica. Ciencia y Tecnología
repository.mail.fl_str_mv
repository.name.fl_str_mv Revista Amazónica. Ciencia y Tecnología - Universidad Estatal Amazónica
repository_id_str
rights_invalid_str_mv Derechos de autor 2017 Revista Amazónica. Ciencia y Tecnología
https://creativecommons.org/licenses/by/4.0
spelling Thyme (Thymus vulgaris) as an antimicrobial agent in the production of fresh cheeseTomillo (Thymus vulgaris) como agente antimicrobiano en la producción de queso frescoMejía López, AnaHerrera, ByronSalazar, MarioRojas Vallejo, María FernandaGavín, VerónicaEscobar Machado, José AntonioQuesoinhibición de Staphylococcus aureustomilloCheeseinhibition of Staphylococcus aureuthymeThe effect of the use of the thyme Thymus vulgaris leaves in the development of. Staphylococcus aureus, a microorganism that is associated with outbreaks of food poisoning and which is commonly present in fresh cheese of the artisanal type, for this purpose, cheese was prepared by inoculating strains of S. aureus ATCC 25923 previously suspended with a turbidity equivalent to the scale of 0.5 McFarland, and was evaluated through a completely randomized design with 3 replicates, where the factors under study were 2 types of processes (1) in sheets incorporated in cheese, (2) in infusion in salty water, and 4 Concentrations (0, 1, 075 and 0.5%). Colony-forming units were determined following the official APHA methodology on days 1, 8, 15, and 30 after their production. As a result, a significant reduction of the microbial load in the experimental plates (1 and 075% with thyme) was obtained in comparison to the control plates (0%) in process 1, whereas there was reduction for all the concentrations tested when added the infused thyme during the salting of the cheese. Concluding that at greater concentration of thyme greater inhibitory effect on S. aureus and could be used in the preservation of food as the main natural antimicrobial compounds in order to ensure the production of microbiologically stable food.Se evaluó el efecto de la utilización de las hojas de tomillo (Thymus vulgaris) en el desarrollo de Stafilococus aureus, microorganismo que está asociado con brotes de intoxicaciones alimentarias y que comúnmente se encuentra presente en queso fresco de tipo artesanal. Para ello se elaboró queso inoculando cepas de S. aureus ATCC 25923 previamente suspendida con una turbidez equivalente a la escala 0.5 de McFarland y se evaluó a través de un diseño completamente al azar con 3 repeticiones, donde los factores en estudio fueron 2 tipos de procesos (1) en hojas incorporadas en el queso, (2) en infusión en el agua de salado y 4 concentraciones (0, 1, 0,75 y 0,5%). Se determinaron las unidades formadores de colonias siguiendo la metodología oficial APHA en los días 1, 8, 15, y 30 después de su producción. Como resultado se obtuvo una reducción significativa de la carga microbiana en las placas experimentales (1 y 0,75% con tomillo) en comparación con las placas de control (0%) en el proceso 1, mientras que existió reducción para todas las concentraciones ensayadas cuando se adicionó el tomillo en infusión durante el salado del queso. Se concluye que a mayor concentración de Tomillo mayor el efecto inhibitorio sobre S.aureus y que podría utilizarse en la conservación de alimentos como principales compuestos antimicrobianos naturales a fin de asegurar la producción de alimentos microbiológicamente estable.Universidad Estatal Amazónica2017-04-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistas.uea.edu.ec/index.php/racyt/article/view/7110.59410/RACYT-v06n01ep05-0071Revista Amazónica. Ciencia y Tecnología; Vol. 6 No. 1 (2017): Revista Amazónica Ciencia y Tecnología ; 45 - 54Revista Amazónica. Ciencia y Tecnología; Vol. 6 Núm. 1 (2017): Revista Amazónica Ciencia y Tecnología ; 45 - 541390-80491390-5600reponame:Revista Amazónica. Ciencia y Tecnologíainstname:Universidad Estatal Amazónicainstacron:UEAspahttps://revistas.uea.edu.ec/index.php/racyt/article/view/71/74https://revistas.uea.edu.ec/index.php/racyt/article/view/71/235Derechos de autor 2017 Revista Amazónica. Ciencia y Tecnologíahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess2023-07-18T20:38:46Zoai:ojs_revista.www.uea.edu.ec:article/71Portal de revistashttps://revistas.uea.edu.ec/index.php/racytUniversidad públicahttp://www.uea.edu.ec/https://revistas.uea.edu.ec/index.php/racyt/oaiEcuadoropendoar:2023-07-18T20:38:46falsePortal de revistashttps://revistas.uea.edu.ec/index.php/racytUniversidad públicahttp://www.uea.edu.ec/https://revistas.uea.edu.ec/index.php/racyt/oaiEcuadoropendoar:2023-07-18T20:38:46Revista Amazónica. Ciencia y Tecnología - Universidad Estatal Amazónicafalse
spellingShingle Thyme (Thymus vulgaris) as an antimicrobial agent in the production of fresh cheese
Mejía López, Ana
Queso
inhibición de Staphylococcus aureus
tomillo
Cheese
inhibition of Staphylococcus aureu
thyme
status_str publishedVersion
title Thyme (Thymus vulgaris) as an antimicrobial agent in the production of fresh cheese
title_full Thyme (Thymus vulgaris) as an antimicrobial agent in the production of fresh cheese
title_fullStr Thyme (Thymus vulgaris) as an antimicrobial agent in the production of fresh cheese
title_full_unstemmed Thyme (Thymus vulgaris) as an antimicrobial agent in the production of fresh cheese
title_short Thyme (Thymus vulgaris) as an antimicrobial agent in the production of fresh cheese
title_sort Thyme (Thymus vulgaris) as an antimicrobial agent in the production of fresh cheese
topic Queso
inhibición de Staphylococcus aureus
tomillo
Cheese
inhibition of Staphylococcus aureu
thyme
url https://revistas.uea.edu.ec/index.php/racyt/article/view/71