IMPLEMENTATION OF PCR (CHAIN REACTION IN POLYMERASE) FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS IN ECUADOR

In the present work I implement the technique of PCR for the detection of Brucella abortus, in blood samples, comparison with the Rose of bengal test. The amplification of the DNA was made using three oligonucleotidos homologous corresponding ones to the sequence 16 ARNr de Brucella abortus, giving a 725 amplification of 900 and pb respectively. A total of 172 blood samples was collected of 4 cattle ranches with historical prevalence of brucelosis presence. Were 142 refusals with the test of serológia and 143 with PCR, 30 were positive with the Pink serológica test of Bengal and 29 left positive with PCR. All the animals that left positives with Rose Bengal, 4 presented clinical symptoms as they are; weak abortions, bull calves, low production of meat and milk. The other 26 animals were negative with PCR and these animals did not present/display the clinical symptoms. From the 142 animals that gave negative with Rose of Bengal, 25 were positive with PCR. The results show that the detection of the positive animals by means of the PCR was more you specify and sensible that the serológica test of Rose of Bengal, therefore is a very useful tool in the diagnosis of Brucella abortus.

• Auteur principal: Cevallos Falquez, Orly Fernando (author)
• Autres auteurs: Motte, Emerick (author), Canchignia Martínez, Hayron Fabricio (author), Carranza Patiño, Mercedes Susana (author), Saucedo Aguiar, Silvia Gicela (author), Cedeño, Virna (author)
• Format: article
• Langue: eng
• Publié: 2008
• Sujets: PCR; ROSA DE BENGALA; BRUCELLA ABORTUS; VACUNA CEPA 19 Y BRUCELOSIS.; ROSE OF BENGAL; VACCINE STOCK 19 AND BRUCELOSIS.
• Accès en ligne: https://revistas.uteq.edu.ec/index.php/cyt/article/view/64