Activation of induced systemic resistance in grapevine “Thompson Seedless”, response Pseudomonas veronii R4
The main objective was to evaluate the mechanism of RSI to “Thompson Seedless” for stimulus (Lox2, Tlp1, Npr1, Eir1) genes. Activation of defense genes for leaves and roots were checked, showing the systemic activation by inoculation of P. veronii R4 in ‘Thompson Seedle...
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| Γλώσσα: | eng |
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2016
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| Διαθέσιμο Online: | https://revistas.uteq.edu.ec/index.php/cyt/article/view/161 |
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Προσθήκη ετικέτας | _version_ | 1862931111324155904 |
|---|---|
| author | Peñafiel Jaramillo, Maria |
| author2 | Pinargote, Carlos Belezaca Sánchez-Sepúlveda, Evelyn Canchignia Martínez, Hayron Fabricio Prieto-Encalada, Humberto Cruz Rosero, Nicolás |
| author2_role | author author author author author |
| author_facet | Peñafiel Jaramillo, Maria Pinargote, Carlos Belezaca Sánchez-Sepúlveda, Evelyn Canchignia Martínez, Hayron Fabricio Prieto-Encalada, Humberto Cruz Rosero, Nicolás |
| author_role | author |
| collection | Revista Ciencia y Tecnología |
| dc.creator.none.fl_str_mv | Peñafiel Jaramillo, Maria Pinargote, Carlos Belezaca Sánchez-Sepúlveda, Evelyn Canchignia Martínez, Hayron Fabricio Prieto-Encalada, Humberto Cruz Rosero, Nicolás |
| dc.date.none.fl_str_mv | 2016-05-20 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://revistas.uteq.edu.ec/index.php/cyt/article/view/161 10.18779/cyt.v9i1.161 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Universidad Técnica Estatal de Quevedo |
| dc.relation.none.fl_str_mv | https://revistas.uteq.edu.ec/index.php/cyt/article/view/161/175 |
| dc.rights.none.fl_str_mv | https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | Ciencia y Tecnología; Vol. 9 No. 1 (2016): January-June (2016); 1-9 Ciencia y Tecnología; Vol. 9 Núm. 1 (2016): Enero-Junio (2016); 1-9 Ciencia y Tecnología; v. 9 n. 1 (2016): Enero-Junio (2016); 1-9 1390-4043 1390-4051 10.18779/cyt.v9i1 reponame:Revista Ciencia y Tecnología instname:Universidad Técnica Estatal de Quevedo instacron:UTEQ |
| dc.subject.none.fl_str_mv | ÁCIDO SALICÍLICO ARABIDOPSIS THALIANA JASMONATO VITIS VINÍFERA ALICYLIC ACID ARABIDOPSIS THALIANA JASMONATE VITIS VINÍFERA |
| dc.title.none.fl_str_mv | Activation of induced systemic resistance in grapevine “Thompson Seedless”, response Pseudomonas veronii R4 Activación de resistencia sistémica inducida en vid “Thompson Seedless”, en respuesta Pseudomonas veronii R4 |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | The main objective was to evaluate the mechanism of RSI to “Thompson Seedless” for stimulus (Lox2, Tlp1, Npr1, Eir1) genes. Activation of defense genes for leaves and roots were checked, showing the systemic activation by inoculation of P. veronii R4 in ‘Thompson Seedless’. Observed by q-PCR gene expression Lox2 in vine leaves with progressive increase up (12 h post inoculation) sampling, analyzing this same gene in roots did not exist any stimulus. The transcriptional levels of genes Tlp1 Eir1 and were stimulated only in roots when exposed to R4. Stimulus levels Tlp1 relates gene having R4 skill in symbiosis develop complex induced via the ethylene (ET). Stimulation levels were constituent Npr1 gene in leaves and roots, with no signi cant differences between plants treated with PBS or R4. The results show that R4, contact roots of ‘Thompson Seedless’, stimulate the expression of Eir1, Lox2, Tlp1 genes to 5 min, 6 and 12 h post inoculation, allowing establish the effectiveness of RSI strain led R4 in “Thompson Seedless” giving a state of pre-alert. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | REVCYT_5303a5b2de273e441bd03a3d823f8601 |
| identifier_str_mv | 10.18779/cyt.v9i1.161 |
| instacron_str | UTEQ |
| institution | UTEQ |
| instname_str | Universidad Técnica Estatal de Quevedo |
| language | eng |
| network_acronym_str | REVCYT |
| network_name_str | Revista Ciencia y Tecnología |
| oai_identifier_str | oai:revistas.uteq.edu.ec:article/161 |
| publishDate | 2016 |
| publisher.none.fl_str_mv | Universidad Técnica Estatal de Quevedo |
| reponame_str | Revista Ciencia y Tecnología |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | Revista Ciencia y Tecnología - Universidad Técnica Estatal de Quevedo |
| repository_id_str | |
| rights_invalid_str_mv | https://creativecommons.org/licenses/by-nc-sa/4.0 |
| spelling | Activation of induced systemic resistance in grapevine “Thompson Seedless”, response Pseudomonas veronii R4Activación de resistencia sistémica inducida en vid “Thompson Seedless”, en respuesta Pseudomonas veronii R4Peñafiel Jaramillo, MariaPinargote, Carlos BelezacaSánchez-Sepúlveda, EvelynCanchignia Martínez, Hayron FabricioPrieto-Encalada, HumbertoCruz Rosero, NicolásÁCIDO SALICÍLICOARABIDOPSIS THALIANAJASMONATOVITIS VINÍFERAALICYLIC ACIDARABIDOPSIS THALIANAJASMONATEVITIS VINÍFERAThe main objective was to evaluate the mechanism of RSI to “Thompson Seedless” for stimulus (Lox2, Tlp1, Npr1, Eir1) genes. Activation of defense genes for leaves and roots were checked, showing the systemic activation by inoculation of P. veronii R4 in ‘Thompson Seedless’. Observed by q-PCR gene expression Lox2 in vine leaves with progressive increase up (12 h post inoculation) sampling, analyzing this same gene in roots did not exist any stimulus. The transcriptional levels of genes Tlp1 Eir1 and were stimulated only in roots when exposed to R4. Stimulus levels Tlp1 relates gene having R4 skill in symbiosis develop complex induced via the ethylene (ET). Stimulation levels were constituent Npr1 gene in leaves and roots, with no signi cant differences between plants treated with PBS or R4. The results show that R4, contact roots of ‘Thompson Seedless’, stimulate the expression of Eir1, Lox2, Tlp1 genes to 5 min, 6 and 12 h post inoculation, allowing establish the effectiveness of RSI strain led R4 in “Thompson Seedless” giving a state of pre-alert. El objetivo principal fue evaluar el mecanismo de RSI en “Thompson Seedless”, al estímulo de los genes (Lox2, Tlp1, Npr1, Eir1). Se verifico la activación de genes de defensa para hojas y raíces, demostrando la activación sistémica por inoculación de P. veronii R4 en ‘Thompson Seedless’. Mediante q-PCR observamos la expresión del gen Lox2 en hojas de vid, con aumento progresivo hasta (12 h de post inoculación) de muestreo, al analizar este mismo gen en raíces no existió estímulo alguno. Los niveles transcripcionales de los genes Eir1 y Tlp1, fueron estimulados solo en raíces al ser expuestas por R4. Los niveles de estímulo del gen Tlp1 se relaciona con la habilidad que tiene R4 en desarrollar el complejo de simbiosis inducido por la vía del etileno (ET). Los niveles de estímulo del gen Npr1 fueron constitutivos en hojas y raíces, no encontrando diferencias signi cativas entre plantas tratadas con PBS o R4. Los resultados demuestran que R4, en contacto con raíces de ‘Thompson Seedless’, estimulan la expresión de los genes Eir1, Lox2, Tlp1 a 5 min, 6 y 12 h de post inoculación, permitiendo establecer la efectividad de RSI dirigida por la cepa R4 en “Thompson Seedless” con riendo un estado de prealerta. Universidad Técnica Estatal de Quevedo2016-05-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.uteq.edu.ec/index.php/cyt/article/view/16110.18779/cyt.v9i1.161Ciencia y Tecnología; Vol. 9 No. 1 (2016): January-June (2016); 1-9Ciencia y Tecnología; Vol. 9 Núm. 1 (2016): Enero-Junio (2016); 1-9Ciencia y Tecnología; v. 9 n. 1 (2016): Enero-Junio (2016); 1-91390-40431390-405110.18779/cyt.v9i1reponame:Revista Ciencia y Tecnologíainstname:Universidad Técnica Estatal de Quevedoinstacron:UTEQenghttps://revistas.uteq.edu.ec/index.php/cyt/article/view/161/175https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccess2020-06-30T04:39:16Zoai:revistas.uteq.edu.ec:article/161Portal de revistashttps://revistas.uteq.edu.ec/index.php/cytUniversidad públicahttps://www.uteq.edu.ecEcuadorCiencia y tecnología1390-40431390-4043opendoar:2020-06-30T04:39:16falsePortal de revistashttps://revistas.uteq.edu.ec/index.php/cytUniversidad públicahttps://www.uteq.edu.ecEcuadorCiencia y tecnología1390-40431390-4043opendoar:2020-06-30T04:39:16Revista Ciencia y Tecnología - Universidad Técnica Estatal de Quevedofalse |
| spellingShingle | Activation of induced systemic resistance in grapevine “Thompson Seedless”, response Pseudomonas veronii R4 Peñafiel Jaramillo, Maria ÁCIDO SALICÍLICO ARABIDOPSIS THALIANA JASMONATO VITIS VINÍFERA ALICYLIC ACID ARABIDOPSIS THALIANA JASMONATE VITIS VINÍFERA |
| status_str | publishedVersion |
| title | Activation of induced systemic resistance in grapevine “Thompson Seedless”, response Pseudomonas veronii R4 |
| title_full | Activation of induced systemic resistance in grapevine “Thompson Seedless”, response Pseudomonas veronii R4 |
| title_fullStr | Activation of induced systemic resistance in grapevine “Thompson Seedless”, response Pseudomonas veronii R4 |
| title_full_unstemmed | Activation of induced systemic resistance in grapevine “Thompson Seedless”, response Pseudomonas veronii R4 |
| title_short | Activation of induced systemic resistance in grapevine “Thompson Seedless”, response Pseudomonas veronii R4 |
| title_sort | Activation of induced systemic resistance in grapevine “Thompson Seedless”, response Pseudomonas veronii R4 |
| topic | ÁCIDO SALICÍLICO ARABIDOPSIS THALIANA JASMONATO VITIS VINÍFERA ALICYLIC ACID ARABIDOPSIS THALIANA JASMONATE VITIS VINÍFERA |
| url | https://revistas.uteq.edu.ec/index.php/cyt/article/view/161 |