SEROLOGICAL DIAGNOSIS (ROSE BENGAL) AND MOLECULAR (PCR) OF BRUCELLOSIS IN HUMAN
The objective of this research was: To determine the presence of brucellosis in the personnel working in the slaughterhouses of the cantons, Buena Fe, Quevedo, El Empalme and Pichincha, the serological test using Rose Bengal (RB) and molecular techniques ( PCR). Was used as a source of peripheral bl...
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| Ձևաչափ: | article |
| Լեզու: | eng |
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2010
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| Առցանց հասանելիություն: | https://revistas.uteq.edu.ec/index.php/cyt/article/view/86 |
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Ավելացրեք ցուցիչ | _version_ | 1862931111035797504 |
|---|---|
| author | Cevallos Falquez, Orly Fernando |
| author2 | Rodríguez Grefa, Aldelmo Escobar Troya, Ariel Mestanza Uquillas, Camilo Romero Garaicoa, Diego Canchignia Martínez, Fabricio Vera Chang, Jaime Fabian Mariscal Álvarez, Jonathan Cobeña Rosado, Ketty Ramos Gavilanes, Luís Lorena Cadme, Maria Carranza Patiño, Mercedes Susana Saucedo Aguiar, Silvia Gicela Reyes Chancay, Ximena |
| author2_role | author author author author author author author author author author author author author |
| author_facet | Cevallos Falquez, Orly Fernando Rodríguez Grefa, Aldelmo Escobar Troya, Ariel Mestanza Uquillas, Camilo Romero Garaicoa, Diego Canchignia Martínez, Fabricio Vera Chang, Jaime Fabian Mariscal Álvarez, Jonathan Cobeña Rosado, Ketty Ramos Gavilanes, Luís Lorena Cadme, Maria Carranza Patiño, Mercedes Susana Saucedo Aguiar, Silvia Gicela Reyes Chancay, Ximena |
| author_role | author |
| collection | Revista Ciencia y Tecnología |
| dc.creator.none.fl_str_mv | Cevallos Falquez, Orly Fernando Rodríguez Grefa, Aldelmo Escobar Troya, Ariel Mestanza Uquillas, Camilo Romero Garaicoa, Diego Canchignia Martínez, Fabricio Vera Chang, Jaime Fabian Mariscal Álvarez, Jonathan Cobeña Rosado, Ketty Ramos Gavilanes, Luís Lorena Cadme, Maria Carranza Patiño, Mercedes Susana Saucedo Aguiar, Silvia Gicela Reyes Chancay, Ximena |
| dc.date.none.fl_str_mv | 2010-06-30 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://revistas.uteq.edu.ec/index.php/cyt/article/view/86 10.18779/cyt.v3i1.86 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Universidad Técnica Estatal de Quevedo |
| dc.relation.none.fl_str_mv | https://revistas.uteq.edu.ec/index.php/cyt/article/view/86/100 |
| dc.rights.none.fl_str_mv | https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | Ciencia y Tecnología; Vol. 3 No. 1 (2010): January-June (2010); 27-32 Ciencia y Tecnología; Vol. 3 Núm. 1 (2010): Enero-Junio (2010); 27-32 Ciencia y Tecnología; v. 3 n. 1 (2010): Enero-Junio (2010); 27-32 1390-4043 1390-4051 10.18779/cyt.v3i1 reponame:Revista Ciencia y Tecnología instname:Universidad Técnica Estatal de Quevedo instacron:UTEQ |
| dc.subject.none.fl_str_mv | BRUCELLAS SEROLÓGICA SANGRE PERIFÉRICA ECUADOR. BRUCELLA SEROLOGY PERIPHERAL BLOOD ECUADOR. |
| dc.title.none.fl_str_mv | SEROLOGICAL DIAGNOSIS (ROSE BENGAL) AND MOLECULAR (PCR) OF BRUCELLOSIS IN HUMAN DIAGNÓSTICO SEROLÓGICO (ROSA DE BENGALA) Y MOLECULAR (PCR) DE BRUCELOSIS EN HUMANO |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | The objective of this research was: To determine the presence of brucellosis in the personnel working in the slaughterhouses of the cantons, Buena Fe, Quevedo, El Empalme and Pichincha, the serological test using Rose Bengal (RB) and molecular techniques ( PCR). Was used as a source of peripheral blood DNA and antibodies. Of a total of 115 blood samples collected at staff working in the slaughterhouses and slaughterers and operators, 54 (47%) and 15 (13%) were PCR positive and RB respectively, giving 61 (53%) and 100 (87%) negative for both tests accordingly. The average number of positive cases for the three populations except Pichincha by PCR was 3.4% and 39.4% RB. It takes care with the Pichincha Canton because 19 (70.3%) of samples were positive with RB of which 12 (44.4%) when analyzed with PCR were negative, and showed a 52.6% , indicating a high incidence of the disease. As long as the negative sample RB 8, 2 were PCR positive giving a correlation between negative 75%. The positive samples amplified a fragment of 725 pb. This work suggests that PCR is a highly efficient tool and very useful for diagnosis of brucellosis in humans. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | REVCYT_b9be4abee2489320f2243e90e02e4e40 |
| identifier_str_mv | 10.18779/cyt.v3i1.86 |
| instacron_str | UTEQ |
| institution | UTEQ |
| instname_str | Universidad Técnica Estatal de Quevedo |
| language | eng |
| network_acronym_str | REVCYT |
| network_name_str | Revista Ciencia y Tecnología |
| oai_identifier_str | oai:revistas.uteq.edu.ec:article/86 |
| publishDate | 2010 |
| publisher.none.fl_str_mv | Universidad Técnica Estatal de Quevedo |
| reponame_str | Revista Ciencia y Tecnología |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | Revista Ciencia y Tecnología - Universidad Técnica Estatal de Quevedo |
| repository_id_str | |
| rights_invalid_str_mv | https://creativecommons.org/licenses/by-nc-sa/4.0 |
| spelling | SEROLOGICAL DIAGNOSIS (ROSE BENGAL) AND MOLECULAR (PCR) OF BRUCELLOSIS IN HUMANDIAGNÓSTICO SEROLÓGICO (ROSA DE BENGALA) Y MOLECULAR (PCR) DE BRUCELOSIS EN HUMANOCevallos Falquez, Orly FernandoRodríguez Grefa, AldelmoEscobar Troya, ArielMestanza Uquillas, CamiloRomero Garaicoa, DiegoCanchignia Martínez, FabricioVera Chang, Jaime FabianMariscal Álvarez, JonathanCobeña Rosado, KettyRamos Gavilanes, LuísLorena Cadme, MariaCarranza Patiño, Mercedes SusanaSaucedo Aguiar, Silvia GicelaReyes Chancay, XimenaBRUCELLASSEROLÓGICASANGRE PERIFÉRICAECUADOR.BRUCELLASEROLOGYPERIPHERAL BLOODECUADOR.The objective of this research was: To determine the presence of brucellosis in the personnel working in the slaughterhouses of the cantons, Buena Fe, Quevedo, El Empalme and Pichincha, the serological test using Rose Bengal (RB) and molecular techniques ( PCR). Was used as a source of peripheral blood DNA and antibodies. Of a total of 115 blood samples collected at staff working in the slaughterhouses and slaughterers and operators, 54 (47%) and 15 (13%) were PCR positive and RB respectively, giving 61 (53%) and 100 (87%) negative for both tests accordingly. The average number of positive cases for the three populations except Pichincha by PCR was 3.4% and 39.4% RB. It takes care with the Pichincha Canton because 19 (70.3%) of samples were positive with RB of which 12 (44.4%) when analyzed with PCR were negative, and showed a 52.6% , indicating a high incidence of the disease. As long as the negative sample RB 8, 2 were PCR positive giving a correlation between negative 75%. The positive samples amplified a fragment of 725 pb. This work suggests that PCR is a highly efficient tool and very useful for diagnosis of brucellosis in humans.El objetivo de esta investigación fue: Determinar y comparar la presencia de Brucelosis del personal que labora en los camales de los cantones, Buena Fé, Quevedo, El Empalme y Pichincha, utilizando la prueba serológica Rosa de Bengala (RB) y la técnica molecular (PCR). Se utilizó como fuente de ADN y anticuerpos sangre periférica. De un total de 115 muestras de sangre recolectadas al personal que labora en los camales como faenadores y operadores, 54 (47%) y 15 (13%) fueron positivas con RB y PCR respectivamente, dando 61(53%) y 100 (87%) negativas para las dos pruebas correspondientemente. El promedio de casos positivos para las tres poblaciones excepto Pichincha por PCR fue de 3.4% y para RB 39.4%. Se toma atención con el cantón Pichincha debido a que 19 (70.3%) de las muestras salieron positivas con RB de las cuales 12 (44.4%) al ser analizadas con PCR fueron negativas, teniendo una correlación de 52.6%, lo que indica una alta incidencia de la enfermedad. Mientras tanto que de las 8 muestras negativas con RB, 2 fueron positivas con PCR dando una correlación entre negativos del 75%. Las muestras positivas amplificaron un fragmento de 725 pb. Con este trabajo se sugiere que la técnica de PCR es una herramienta altamente eficiente y muy útil para el diagnóstico de la brucelosis en humanos.Universidad Técnica Estatal de Quevedo2010-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.uteq.edu.ec/index.php/cyt/article/view/8610.18779/cyt.v3i1.86Ciencia y Tecnología; Vol. 3 No. 1 (2010): January-June (2010); 27-32Ciencia y Tecnología; Vol. 3 Núm. 1 (2010): Enero-Junio (2010); 27-32Ciencia y Tecnología; v. 3 n. 1 (2010): Enero-Junio (2010); 27-321390-40431390-405110.18779/cyt.v3i1reponame:Revista Ciencia y Tecnologíainstname:Universidad Técnica Estatal de Quevedoinstacron:UTEQenghttps://revistas.uteq.edu.ec/index.php/cyt/article/view/86/100https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccess2020-06-30T04:50:41Zoai:revistas.uteq.edu.ec:article/86Portal de revistashttps://revistas.uteq.edu.ec/index.php/cytUniversidad públicahttps://www.uteq.edu.ecEcuadorCiencia y tecnología1390-40431390-4043opendoar:2020-06-30T04:50:41falsePortal de revistashttps://revistas.uteq.edu.ec/index.php/cytUniversidad públicahttps://www.uteq.edu.ecEcuadorCiencia y tecnología1390-40431390-4043opendoar:2020-06-30T04:50:41Revista Ciencia y Tecnología - Universidad Técnica Estatal de Quevedofalse |
| spellingShingle | SEROLOGICAL DIAGNOSIS (ROSE BENGAL) AND MOLECULAR (PCR) OF BRUCELLOSIS IN HUMAN Cevallos Falquez, Orly Fernando BRUCELLAS SEROLÓGICA SANGRE PERIFÉRICA ECUADOR. BRUCELLA SEROLOGY PERIPHERAL BLOOD ECUADOR. |
| status_str | publishedVersion |
| title | SEROLOGICAL DIAGNOSIS (ROSE BENGAL) AND MOLECULAR (PCR) OF BRUCELLOSIS IN HUMAN |
| title_full | SEROLOGICAL DIAGNOSIS (ROSE BENGAL) AND MOLECULAR (PCR) OF BRUCELLOSIS IN HUMAN |
| title_fullStr | SEROLOGICAL DIAGNOSIS (ROSE BENGAL) AND MOLECULAR (PCR) OF BRUCELLOSIS IN HUMAN |
| title_full_unstemmed | SEROLOGICAL DIAGNOSIS (ROSE BENGAL) AND MOLECULAR (PCR) OF BRUCELLOSIS IN HUMAN |
| title_short | SEROLOGICAL DIAGNOSIS (ROSE BENGAL) AND MOLECULAR (PCR) OF BRUCELLOSIS IN HUMAN |
| title_sort | SEROLOGICAL DIAGNOSIS (ROSE BENGAL) AND MOLECULAR (PCR) OF BRUCELLOSIS IN HUMAN |
| topic | BRUCELLAS SEROLÓGICA SANGRE PERIFÉRICA ECUADOR. BRUCELLA SEROLOGY PERIPHERAL BLOOD ECUADOR. |
| url | https://revistas.uteq.edu.ec/index.php/cyt/article/view/86 |