Effect of Ruta graveolens (Rue) essential oil in high concentrations on Staphylococcus aureus subsp aureus strain ATCC® 25904

The objective of the research was to evaluate the antimicrobial efficacy of the essential oil of ruda (Ruta graveolens) onStaphylococcus aureus subp aureus strain ATCC ®25904. the plant was collected from the Inapisí sector of Constantino Fernández, the oil was obtained with the hydrodistillation te...

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1. autor: Montero Recalde, Mayra Andrea (author)
Kolejni autorzy: Aroca Izurieta, Carlos Enrique (author), Aroca Tirado, Álvaro Jeanpool (author), Chuquimarca Chuquimarca, María Angélica (author), Pujos , Julio César (author), Guevara , Esteban Santiago (author)
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Wydane: 2025
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Dostęp online:https://revistas.uteq.edu.ec/index.php/cyt/article/view/790
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author Montero Recalde, Mayra Andrea
author2 Aroca Izurieta, Carlos Enrique
Aroca Tirado, Álvaro Jeanpool
Chuquimarca Chuquimarca, María Angélica
Pujos , Julio César
Guevara , Esteban Santiago
author2_role author
author
author
author
author
author_facet Montero Recalde, Mayra Andrea
Aroca Izurieta, Carlos Enrique
Aroca Tirado, Álvaro Jeanpool
Chuquimarca Chuquimarca, María Angélica
Pujos , Julio César
Guevara , Esteban Santiago
author_role author
collection Revista Ciencia y Tecnología
dc.creator.none.fl_str_mv Montero Recalde, Mayra Andrea
Aroca Izurieta, Carlos Enrique
Aroca Tirado, Álvaro Jeanpool
Chuquimarca Chuquimarca, María Angélica
Pujos , Julio César
Guevara , Esteban Santiago
dc.date.none.fl_str_mv 2025-01-15
dc.format.none.fl_str_mv application/pdf
text/html
dc.identifier.none.fl_str_mv https://revistas.uteq.edu.ec/index.php/cyt/article/view/790
10.18779/cyt.v18i1.790
dc.language.none.fl_str_mv spa
dc.publisher.none.fl_str_mv Universidad Técnica Estatal de Quevedo
dc.relation.none.fl_str_mv https://revistas.uteq.edu.ec/index.php/cyt/article/view/790/985
https://revistas.uteq.edu.ec/index.php/cyt/article/view/790/986
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
dc.source.none.fl_str_mv Ciencia y Tecnología; Vol. 18 No. 1 (2025): Science and Technology Journal; 1-7
Ciencia y Tecnología; Vol. 18 Núm. 1 (2025): Revista Ciencia y Tecnología ; 1-7
Ciencia y Tecnología; v. 18 n. 1 (2025): Revista Ciência e Tecnologia; 1-7
1390-4043
1390-4051
10.18779/cyt.v18i1
reponame:Revista Ciencia y Tecnología
instname:Universidad Técnica Estatal de Quevedo
instacron:UTEQ
dc.subject.none.fl_str_mv sensibilidad antimicrobiana
concentración mínima inhibitoria
concentración mínima bactericida
hidrodestilación
antimicrobial sensitivity
minimum inhibitory concentration
minimal bactericidal concentration
hydrodistillation
dc.title.none.fl_str_mv Effect of Ruta graveolens (Rue) essential oil in high concentrations on Staphylococcus aureus subsp aureus strain ATCC® 25904
Efecto del aceite esencial de Ruta graveolens (Ruda) en altas concentraciones sobre Staphylococcus aureus subesp aureus cepa ATCC® 25904
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
description The objective of the research was to evaluate the antimicrobial efficacy of the essential oil of ruda (Ruta graveolens) onStaphylococcus aureus subp aureus strain ATCC ®25904. the plant was collected from the Inapisí sector of Constantino Fernández, the oil was obtained with the hydrodistillation technician, 10 concentrations were evaluated (10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100% ) in dilution with double distilled water, each dilution was handled a total of 5 ml and tween 80 was added as 0,1% as emulsifier, the results showed that the determined minimum inhibitory concentration of the essential oil was defined at 30%; with respect to the Bactericidal minimum concentration evidenced by the zero growth of colonies on the plate, which was established at 40% concentration; the antimicrobial sensitivity study statistically show significant differences between treatments with the 0,5% Tukey test, the best being at 90% concentration with 25,25 mm of inhibition halo formation, however the treatments starting at 30% and showed significant halos formation, concluding that Ruta graveolens oil has antimicrobial efficacy on the strain evaluate. 
eu_rights_str_mv openAccess
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identifier_str_mv 10.18779/cyt.v18i1.790
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instname_str Universidad Técnica Estatal de Quevedo
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oai_identifier_str oai:revistas.uteq.edu.ec:article/790
publishDate 2025
publisher.none.fl_str_mv Universidad Técnica Estatal de Quevedo
reponame_str Revista Ciencia y Tecnología
repository.mail.fl_str_mv
repository.name.fl_str_mv Revista Ciencia y Tecnología - Universidad Técnica Estatal de Quevedo
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
spelling Effect of Ruta graveolens (Rue) essential oil in high concentrations on Staphylococcus aureus subsp aureus strain ATCC® 25904 Efecto del aceite esencial de Ruta graveolens (Ruda) en altas concentraciones sobre Staphylococcus aureus subesp aureus cepa ATCC® 25904 Montero Recalde, Mayra AndreaAroca Izurieta, Carlos Enrique Aroca Tirado, Álvaro JeanpoolChuquimarca Chuquimarca, María Angélica Pujos , Julio César Guevara , Esteban Santiagosensibilidad antimicrobianaconcentración mínima inhibitoriaconcentración mínima bactericidahidrodestilaciónantimicrobial sensitivityminimum inhibitory concentrationminimal bactericidal concentrationhydrodistillationThe objective of the research was to evaluate the antimicrobial efficacy of the essential oil of ruda (Ruta graveolens) onStaphylococcus aureus subp aureus strain ATCC ®25904. the plant was collected from the Inapisí sector of Constantino Fernández, the oil was obtained with the hydrodistillation technician, 10 concentrations were evaluated (10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100% ) in dilution with double distilled water, each dilution was handled a total of 5 ml and tween 80 was added as 0,1% as emulsifier, the results showed that the determined minimum inhibitory concentration of the essential oil was defined at 30%; with respect to the Bactericidal minimum concentration evidenced by the zero growth of colonies on the plate, which was established at 40% concentration; the antimicrobial sensitivity study statistically show significant differences between treatments with the 0,5% Tukey test, the best being at 90% concentration with 25,25 mm of inhibition halo formation, however the treatments starting at 30% and showed significant halos formation, concluding that Ruta graveolens oil has antimicrobial efficacy on the strain evaluate. La investigación tuvo como objetivo evaluar la eficacia antimicrobiana del aceite esencial de ruda (Ruta graveolens) sobre Staphylococcus aureus subesp aureus cepa ATCC ®25904. La planta fue recolectada del sector de Inapisí de la parroquia de Constantino Fernández, el aceite obtenido fue a través del método de hidrodestilación, se evaluaron 10 concentraciones (10%, 20%, 30%, 40%,50%, 60%, 70%, 80%, 90%, 100%) en dilución con agua bidestilada, cada dilución se manejó con un total de 5 ml y se adicionó tween 80 como emulsificador al 0,1%. Los resultados demostraron que la concentración mínima inhibitoria determinada del aceite esencial se definió en el 30% y con respecto a la concentración mínima bactericida evidenciada por el cero crecimiento de colonias en placa, la cual se la estableció al 40% de concentración; el estudio de sensibilidad antimicrobiana precisó estadísticamente con la prueba de Tukey al 0,5%  diferencias significativas entre tratamientos, siendo el mejor al 90% de concentración con 25,25 mm de formación de halos de inhibición, no obstante los tratamientos a partir del 30% presentaron formación de halos de manera importante, concluyendo que el aceite esencial de Ruta graveolens posee eficacia antimicrobiana sobre la cepa evaluada a partir del 30% de concentración. Universidad Técnica Estatal de Quevedo2025-01-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistas.uteq.edu.ec/index.php/cyt/article/view/79010.18779/cyt.v18i1.790Ciencia y Tecnología; Vol. 18 No. 1 (2025): Science and Technology Journal; 1-7Ciencia y Tecnología; Vol. 18 Núm. 1 (2025): Revista Ciencia y Tecnología ; 1-7Ciencia y Tecnología; v. 18 n. 1 (2025): Revista Ciência e Tecnologia; 1-71390-40431390-405110.18779/cyt.v18i1reponame:Revista Ciencia y Tecnologíainstname:Universidad Técnica Estatal de Quevedoinstacron:UTEQspahttps://revistas.uteq.edu.ec/index.php/cyt/article/view/790/985https://revistas.uteq.edu.ec/index.php/cyt/article/view/790/986Derechos de autor 2025 Mayra Andrea Montero Recalde, Carlos Enrique Aroca Izurieta, Álvaro Jeanpool Aroca Tirado, María Angélica Chuquimarca Chuquimarca, Julio César Pujos, Esteban Santiago Guevarahttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccess2025-07-02T15:17:04Zoai:revistas.uteq.edu.ec:article/790Portal de revistashttps://revistas.uteq.edu.ec/index.php/cytUniversidad públicahttps://www.uteq.edu.ecEcuadorCiencia y tecnología1390-40431390-4043opendoar:2025-07-02T15:17:04falsePortal de revistashttps://revistas.uteq.edu.ec/index.php/cytUniversidad públicahttps://www.uteq.edu.ecEcuadorCiencia y tecnología1390-40431390-4043opendoar:2025-07-02T15:17:04Revista Ciencia y Tecnología - Universidad Técnica Estatal de Quevedofalse
spellingShingle Effect of Ruta graveolens (Rue) essential oil in high concentrations on Staphylococcus aureus subsp aureus strain ATCC® 25904
Montero Recalde, Mayra Andrea
sensibilidad antimicrobiana
concentración mínima inhibitoria
concentración mínima bactericida
hidrodestilación
antimicrobial sensitivity
minimum inhibitory concentration
minimal bactericidal concentration
hydrodistillation
status_str publishedVersion
title Effect of Ruta graveolens (Rue) essential oil in high concentrations on Staphylococcus aureus subsp aureus strain ATCC® 25904
title_full Effect of Ruta graveolens (Rue) essential oil in high concentrations on Staphylococcus aureus subsp aureus strain ATCC® 25904
title_fullStr Effect of Ruta graveolens (Rue) essential oil in high concentrations on Staphylococcus aureus subsp aureus strain ATCC® 25904
title_full_unstemmed Effect of Ruta graveolens (Rue) essential oil in high concentrations on Staphylococcus aureus subsp aureus strain ATCC® 25904
title_short Effect of Ruta graveolens (Rue) essential oil in high concentrations on Staphylococcus aureus subsp aureus strain ATCC® 25904
title_sort Effect of Ruta graveolens (Rue) essential oil in high concentrations on Staphylococcus aureus subsp aureus strain ATCC® 25904
topic sensibilidad antimicrobiana
concentración mínima inhibitoria
concentración mínima bactericida
hidrodestilación
antimicrobial sensitivity
minimum inhibitory concentration
minimal bactericidal concentration
hydrodistillation
url https://revistas.uteq.edu.ec/index.php/cyt/article/view/790