Characterization of proteins obtained from three dairy products developed in the Salinas agricultural production cooperative

The objective of this research is the protein characterization of three milk beverages that are made from sweet whey with protein contributions from two vegetable matrices (quinoa-based milk drink, soy-based milk drink and fermented milk drink), to The research concentrated the protein by isoelectri...

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Glavni autor: Altuna, José (author)
Daljnji autori: Barrionuevo, Alejandra (author), Bayas, Favian (author), Verdezoto, Roberto (author), Coloma, Alejandra (author)
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Izdano: 2021
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Online pristup:https://revistas.uteq.edu.ec/index.php/cyt/article/view/506
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author Altuna, José
author2 Barrionuevo, Alejandra
Bayas, Favian
Verdezoto, Roberto
Coloma, Alejandra
author2_role author
author
author
author
author_facet Altuna, José
Barrionuevo, Alejandra
Bayas, Favian
Verdezoto, Roberto
Coloma, Alejandra
author_role author
collection Revista Ciencia y Tecnología
dc.creator.none.fl_str_mv Altuna, José
Barrionuevo, Alejandra
Bayas, Favian
Verdezoto, Roberto
Coloma, Alejandra
dc.date.none.fl_str_mv 2021-12-31
dc.format.none.fl_str_mv application/pdf
text/html
dc.identifier.none.fl_str_mv https://revistas.uteq.edu.ec/index.php/cyt/article/view/506
10.18779/cyt.v14i2.506
dc.language.none.fl_str_mv spa
dc.publisher.none.fl_str_mv Universidad Técnica Estatal de Quevedo
dc.relation.none.fl_str_mv https://revistas.uteq.edu.ec/index.php/cyt/article/view/506/567
https://revistas.uteq.edu.ec/index.php/cyt/article/view/506/589
dc.rights.none.fl_str_mv Derechos de autor 2022 Ciencia y Tecnología
info:eu-repo/semantics/openAccess
dc.source.none.fl_str_mv Ciencia y Tecnología; Vol. 14 No. 2 (2021): Science and Technology Journal July - December 2021; 77-80
Ciencia y Tecnología; Vol. 14 Núm. 2 (2021): Revista Ciencia y Tecnología Julio - Diciembre 2021; 77-80
Ciencia y Tecnología; v. 14 n. 2 (2021): Revista Ciencia y Tecnología Julio - Diciembre 2021; 77-80
1390-4043
1390-4051
10.18779/cyt.v14i2
reponame:Revista Ciencia y Tecnología
instname:Universidad Técnica Estatal de Quevedo
instacron:UTEQ
dc.subject.none.fl_str_mv lacto suero
isoeléctrica
electroforesis
digestión gástrica
digestión duodenal
serum lacto
isoelectric
electrophoresis
gastric digestion
duodenal digestion
dc.title.none.fl_str_mv Characterization of proteins obtained from three dairy products developed in the Salinas agricultural production cooperative
Caracterización de proteínas obtenidas de tres productos lácteos desarrollados en la cooperativa de producción agropecuaria salinas
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
description The objective of this research is the protein characterization of three milk beverages that are made from sweet whey with protein contributions from two vegetable matrices (quinoa-based milk drink, soy-based milk drink and fermented milk drink), to The research concentrated the protein by isoelectric precipitation at pH 4.0 values without solubility, and by means of electrophoresis we identified the protein profile of milk drinks, finding bands with molecular weights between 16 and 85 KDa in the fermented milk drink and the milk-based drink. of soy, and 16 to 79 KDa in the quinoa-based milk drink. Gastrointestinal digestibility was established where gastric digestion with the enzyme pepsin was developed, and duodenal digestion with the enzyme pancreatin, after the enzymatic reaction, the electrophoresis technique was applied and it was verified that each of the milk beverages is digestible within the organism. This research seeks to strengthen the development of local agribusiness and above all to demonstrate that the three dairy drinks developed are easily digested and therefore suitable for human consumption and their proteins described are an important contribution to the nutrition of the body. in such a way that the release of peptides, together with their size, hydrophobicity and sequence, constitute essential requirements to convert them into peptides with biological activity. The latter, also considered as bioactive peptides or biopeptides, have recently aroused the interest of researchers because they can favorably influence the functions carried out by the immune, gastrointestinal, nervous and cardiovascular systems, impacting consumer health.
eu_rights_str_mv openAccess
format article
id REVCYT_f05668dcb06caa930d2e9bf7afe765a8
identifier_str_mv 10.18779/cyt.v14i2.506
instacron_str UTEQ
institution UTEQ
instname_str Universidad Técnica Estatal de Quevedo
language spa
network_acronym_str REVCYT
network_name_str Revista Ciencia y Tecnología
oai_identifier_str oai:revistas.uteq.edu.ec:article/506
publishDate 2021
publisher.none.fl_str_mv Universidad Técnica Estatal de Quevedo
reponame_str Revista Ciencia y Tecnología
repository.mail.fl_str_mv
repository.name.fl_str_mv Revista Ciencia y Tecnología - Universidad Técnica Estatal de Quevedo
repository_id_str
rights_invalid_str_mv Derechos de autor 2022 Ciencia y Tecnología
spelling Characterization of proteins obtained from three dairy products developed in the Salinas agricultural production cooperativeCaracterización de proteínas obtenidas de tres productos lácteos desarrollados en la cooperativa de producción agropecuaria salinasAltuna, José Barrionuevo, Alejandra Bayas, Favian Verdezoto, Roberto Coloma, Alejandra lacto sueroisoeléctricaelectroforesisdigestión gástricadigestión duodenalserum lactoisoelectricelectrophoresisgastric digestionduodenal digestionThe objective of this research is the protein characterization of three milk beverages that are made from sweet whey with protein contributions from two vegetable matrices (quinoa-based milk drink, soy-based milk drink and fermented milk drink), to The research concentrated the protein by isoelectric precipitation at pH 4.0 values without solubility, and by means of electrophoresis we identified the protein profile of milk drinks, finding bands with molecular weights between 16 and 85 KDa in the fermented milk drink and the milk-based drink. of soy, and 16 to 79 KDa in the quinoa-based milk drink. Gastrointestinal digestibility was established where gastric digestion with the enzyme pepsin was developed, and duodenal digestion with the enzyme pancreatin, after the enzymatic reaction, the electrophoresis technique was applied and it was verified that each of the milk beverages is digestible within the organism. This research seeks to strengthen the development of local agribusiness and above all to demonstrate that the three dairy drinks developed are easily digested and therefore suitable for human consumption and their proteins described are an important contribution to the nutrition of the body. in such a way that the release of peptides, together with their size, hydrophobicity and sequence, constitute essential requirements to convert them into peptides with biological activity. The latter, also considered as bioactive peptides or biopeptides, have recently aroused the interest of researchers because they can favorably influence the functions carried out by the immune, gastrointestinal, nervous and cardiovascular systems, impacting consumer health.La presente investigación tiene como objetivo la caracterización proteica de tres bebidas lácteas que son elaboradas a partir de lacto suero dulce con aportes proteicos de dos matrices vegetales (bebida láctea a base de quinua, bebida láctea a base de soya y bebida láctea fermentada), para la investigación se concentró la proteína mediante precipitación isoeléctrica a valores de pH 4.0 sin solubilidad, y mediante electroforesis identificamos el perfil proteico de las bebidas lácteas, encontrándose bandas con pesos moleculares entre 16 a 85 KDa en la bebida láctea fermentada y la bebida láctea a base de soya, y de 16 a 79 KDa en la bebida láctea a base de quinua. Se estableció la digestibilidad gastrointestinal donde se desarrolló la digestión gástrica con la enzima pepsina, y la digestión duodenal con la enzima pancreatina, posterior a la reacción enzimática se aplicó la técnica de electroforesis y se comprobó que cada una de las bebidas lácteas es digerible dentro del organismo. Con esta investigación se busca fortalecer el desarrollo de la agroindustria local y sobre todo demostrar que las tres bebidas lácteas desarrolladas son de fácil digestión y por ende aptas para el consumo humano y sus proteínas descritas son un aporte importante en la nutrición del organismo, de tal forma que la liberación de los péptidos, aunada al tamaño, hidrofobicidad y secuencia de los mismos constituyen requisitos indispensables para convertirlos en péptidos con actividad biológica. Éstos últimos, también considerados como péptidos bioactivos o biopéptidos, han despertado recientemente el interés de los investigadores debido a que pueden influenciar favorablemente las funciones que llevan a cabo los sistemas inmune, gastrointestinal, nervioso y cardiovascular impactando la salud del consumidor.Universidad Técnica Estatal de Quevedo2021-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistas.uteq.edu.ec/index.php/cyt/article/view/50610.18779/cyt.v14i2.506Ciencia y Tecnología; Vol. 14 No. 2 (2021): Science and Technology Journal July - December 2021; 77-80Ciencia y Tecnología; Vol. 14 Núm. 2 (2021): Revista Ciencia y Tecnología Julio - Diciembre 2021; 77-80Ciencia y Tecnología; v. 14 n. 2 (2021): Revista Ciencia y Tecnología Julio - Diciembre 2021; 77-801390-40431390-405110.18779/cyt.v14i2reponame:Revista Ciencia y Tecnologíainstname:Universidad Técnica Estatal de Quevedoinstacron:UTEQspahttps://revistas.uteq.edu.ec/index.php/cyt/article/view/506/567https://revistas.uteq.edu.ec/index.php/cyt/article/view/506/589Derechos de autor 2022 Ciencia y Tecnologíainfo:eu-repo/semantics/openAccess2023-01-26T19:06:28Zoai:revistas.uteq.edu.ec:article/506Portal de revistashttps://revistas.uteq.edu.ec/index.php/cytUniversidad públicahttps://www.uteq.edu.ecEcuadorCiencia y tecnología1390-40431390-4043opendoar:2023-01-26T19:06:28falsePortal de revistashttps://revistas.uteq.edu.ec/index.php/cytUniversidad públicahttps://www.uteq.edu.ecEcuadorCiencia y tecnología1390-40431390-4043opendoar:2023-01-26T19:06:28Revista Ciencia y Tecnología - Universidad Técnica Estatal de Quevedofalse
spellingShingle Characterization of proteins obtained from three dairy products developed in the Salinas agricultural production cooperative
Altuna, José
lacto suero
isoeléctrica
electroforesis
digestión gástrica
digestión duodenal
serum lacto
isoelectric
electrophoresis
gastric digestion
duodenal digestion
status_str publishedVersion
title Characterization of proteins obtained from three dairy products developed in the Salinas agricultural production cooperative
title_full Characterization of proteins obtained from three dairy products developed in the Salinas agricultural production cooperative
title_fullStr Characterization of proteins obtained from three dairy products developed in the Salinas agricultural production cooperative
title_full_unstemmed Characterization of proteins obtained from three dairy products developed in the Salinas agricultural production cooperative
title_short Characterization of proteins obtained from three dairy products developed in the Salinas agricultural production cooperative
title_sort Characterization of proteins obtained from three dairy products developed in the Salinas agricultural production cooperative
topic lacto suero
isoeléctrica
electroforesis
digestión gástrica
digestión duodenal
serum lacto
isoelectric
electrophoresis
gastric digestion
duodenal digestion
url https://revistas.uteq.edu.ec/index.php/cyt/article/view/506