Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador
The thermal waters are not sterile they possess a microbiota reflecting their physicochemical extreme characteristics. There is evidence that this microbiota may be the reservoir of antibiotic resistance genes, termed environmental resistomas. Therefore, the objective of the present work was to stud...
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2016
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| author | Medina Ramírez, Gerardo |
| author2 | Naranjo, Carina Escobar, Sandra Araque, Judith Djabayan, Pablo Andueza, Félix |
| author2_role | author author author author author |
| author_facet | Medina Ramírez, Gerardo Naranjo, Carina Escobar, Sandra Araque, Judith Djabayan, Pablo Andueza, Félix |
| author_role | author |
| collection | Revista FIGEMPA: Investigación y Desarrollo |
| dc.creator.none.fl_str_mv | Medina Ramírez, Gerardo Naranjo, Carina Escobar, Sandra Araque, Judith Djabayan, Pablo Andueza, Félix |
| dc.date.none.fl_str_mv | 2016-12-30 |
| dc.format.none.fl_str_mv | application/pdf text/xml application/zip |
| dc.identifier.none.fl_str_mv | https://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/867 10.29166/revfig.v1i2.867 |
| dc.language.none.fl_str_mv | spa |
| dc.publisher.none.fl_str_mv | Facultad de Ingeniería en Geología, Minas, Petróleos y Ambiental - Universidad Central del Ecuador |
| dc.relation.none.fl_str_mv | https://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/867/874 https://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/867/5484 https://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/867/5483 |
| dc.rights.none.fl_str_mv | https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | FIGEMPA: Investigación y Desarrollo; Vol. 2 No. 2 (2016): WATER; 33 - 37 FIGEMPA: Investigación y Desarrollo; Vol. 2 Núm. 2 (2016): AGUA; 33 - 37 2602-8484 1390-7042 reponame:Revista FIGEMPA: Investigación y Desarrollo instname:Universidad Central del Ecuador instacron:UCE |
| dc.subject.none.fl_str_mv | aguas termales microbiota resistomas hot springs microbiota resistomas |
| dc.title.none.fl_str_mv | Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador Microbiota extremofila y resistomas ambientales de la fuente termal “Termas La Merced” Quito-Ecuador |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | The thermal waters are not sterile they possess a microbiota reflecting their physicochemical extreme characteristics. There is evidence that this microbiota may be the reservoir of antibiotic resistance genes, termed environmental resistomas. Therefore, the objective of the present work was to study the water bacterial microbiota of the Termas la Merced Spa, located near the City of Quito, Ecuador, to isolate the bacteria and determine the resistance patterns to antibiotics. Thermal water samples were collected from both the emergency area and the swimming pools used by bathers for a period of 6 months between July and December 2015. The isolate of the mesophilic aerobic bacteria using the Petrifilm ™ plates. Identification of bacterial colonies was performed according to the tests suggested by MacFaddin (2003) and Barrow and Feltham (1993), supplemented with MicrogenTM bacterial identification tests. Antibiotic susceptibility profiles were performed using the Kirby Bauer method. A total of 13 bacterial clones corresponding to Brevundimonas diminuta, Aeromonas schubertii, Budvicia aquatica, Acinetobacter haemolyticus, Bacillus spp, Citrobacter amalonaticus, Pseudomonas stutzeri and Xenorhabdus beddingii were identified. All isolates showed resistance to at least one antibiotic. The highest number of resistance (4 antibiotics) was exhibited by Brevundimonas diminuta strains. All isolates showed resistance to Cephalothin. It is demonstrated that these waters possess a characteristic microbiota and the presence of environmental resistomas. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | REVFIGEMPA_b5fa91bbfa7f07be434ac533dedaa93a |
| identifier_str_mv | 10.29166/revfig.v1i2.867 |
| instacron_str | UCE |
| institution | UCE |
| instname_str | Universidad Central del Ecuador |
| language | spa |
| network_acronym_str | REVFIGEMPA |
| network_name_str | Revista FIGEMPA: Investigación y Desarrollo |
| oai_identifier_str | oai:revistadigital.uce.edu.ec:article/867 |
| publishDate | 2016 |
| publisher.none.fl_str_mv | Facultad de Ingeniería en Geología, Minas, Petróleos y Ambiental - Universidad Central del Ecuador |
| reponame_str | Revista FIGEMPA: Investigación y Desarrollo |
| repository.mail.fl_str_mv | * |
| repository.name.fl_str_mv | Revista FIGEMPA: Investigación y Desarrollo - Universidad Central del Ecuador |
| repository_id_str | 0 |
| rights_invalid_str_mv | https://creativecommons.org/licenses/by/4.0/ |
| spelling | Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-EcuadorMicrobiota extremofila y resistomas ambientales de la fuente termal “Termas La Merced” Quito-EcuadorMedina Ramírez, GerardoNaranjo, CarinaEscobar, SandraAraque, JudithDjabayan, PabloAndueza, Félixaguas termalesmicrobiotaresistomashot springsmicrobiotaresistomasThe thermal waters are not sterile they possess a microbiota reflecting their physicochemical extreme characteristics. There is evidence that this microbiota may be the reservoir of antibiotic resistance genes, termed environmental resistomas. Therefore, the objective of the present work was to study the water bacterial microbiota of the Termas la Merced Spa, located near the City of Quito, Ecuador, to isolate the bacteria and determine the resistance patterns to antibiotics. Thermal water samples were collected from both the emergency area and the swimming pools used by bathers for a period of 6 months between July and December 2015. The isolate of the mesophilic aerobic bacteria using the Petrifilm ™ plates. Identification of bacterial colonies was performed according to the tests suggested by MacFaddin (2003) and Barrow and Feltham (1993), supplemented with MicrogenTM bacterial identification tests. Antibiotic susceptibility profiles were performed using the Kirby Bauer method. A total of 13 bacterial clones corresponding to Brevundimonas diminuta, Aeromonas schubertii, Budvicia aquatica, Acinetobacter haemolyticus, Bacillus spp, Citrobacter amalonaticus, Pseudomonas stutzeri and Xenorhabdus beddingii were identified. All isolates showed resistance to at least one antibiotic. The highest number of resistance (4 antibiotics) was exhibited by Brevundimonas diminuta strains. All isolates showed resistance to Cephalothin. It is demonstrated that these waters possess a characteristic microbiota and the presence of environmental resistomas.Las aguas termales no son estériles, poseen una microbiota reflejo de sus características fisicoquímicas extremas. Existen evidencias que esta microbiota puede ser el reservorio de genes de resistencia a los antibióticos, denominados resistomas ambientales. Por ello, el objetivo del presente trabajo fue estudiar la microbiota bacteriana del agua del Balneario Termas la Merced, ubicado cerca de la Ciudad de Quito, Ecuador, con el fin de aislar e identificar las bacterias presentes y determinar sus patrones de resistencia a los antibióticos. Se recolectaron muestras de agua termal, tanto de la zona de emergencia, como de las piscinas utilizadas por los bañistas durante los meses de julio a diciembre del 2015. Se realizó el aislamiento bacteriano, utilizando las placas de PetrifilmTM, para bacterias aerobias mesófilas La identificación de las colonias bacterianas se realizó de acuerdo a las pruebas sugeridas por MacFaddin (2003) y Barrow y Feltham (1993), suplementadas con las pruebas de identificación bacteriano MicrogenTM. Los perfiles de susceptibilidad a antibióticos se realizaron utilizando el método de Kirby Bauer. Se identificaron 13 clones bacterianos, correspondientes a las especies Brevundimonas diminuta, Aeromonas schubertii, Budvicia aquatica, Acinetobacter haemolyticus, Bacillus spp, Citrobacter amalonaticus, Pseudomonas stutzeri y Xenorhabdus beddingii. Todos los aislados mostraron resistencia al menos a un antibiótico. El mayor número de resistencias (4 antibióticos) lo exhibieron cepas de la especie Brevundimonas diminuta. Todos los aislados mostraron resistencia a la Cefalotina. Se demuestra que estas aguas poseen una microbiota bacteriana característica y la presencia de resistomas ambientales.Facultad de Ingeniería en Geología, Minas, Petróleos y Ambiental - Universidad Central del Ecuador2016-12-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/xmlapplication/ziphttps://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/86710.29166/revfig.v1i2.867FIGEMPA: Investigación y Desarrollo; Vol. 2 No. 2 (2016): WATER; 33 - 37FIGEMPA: Investigación y Desarrollo; Vol. 2 Núm. 2 (2016): AGUA; 33 - 372602-84841390-7042reponame:Revista FIGEMPA: Investigación y Desarrolloinstname:Universidad Central del Ecuadorinstacron:UCEspahttps://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/867/874https://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/867/5484https://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/867/5483Derechos de autor 2016 Gerardo Medina Ramírez, Carina Naranjo, Sandra Escobar, Judith Araque, Pablo Djabayan, Félix Anduezahttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccess2023-04-10T17:31:47Zoai:revistadigital.uce.edu.ec:article/867Portal de revistashttps://revistadigital.uce.edu.ec/Universidad públicahttps://uce.edu.ec/**Ecuador*602-84841390-7042opendoar:02023-04-10T17:31:47Revista FIGEMPA: Investigación y Desarrollo - Universidad Central del Ecuadorfalse |
| spellingShingle | Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador Medina Ramírez, Gerardo aguas termales microbiota resistomas hot springs microbiota resistomas |
| status_str | publishedVersion |
| title | Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador |
| title_full | Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador |
| title_fullStr | Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador |
| title_full_unstemmed | Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador |
| title_short | Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador |
| title_sort | Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador |
| topic | aguas termales microbiota resistomas hot springs microbiota resistomas |
| url | https://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/867 |