Metabolome analysis in the microbial antagonism by liquid chromatography coupled with chemometrics algorithms

Metabolome is a group of low molecular weight organic compounds (metabolites) produced by biological systems. Bacterial antagonism is an important evolving force, in this sense analysis of its metabolome represents a useful tool for discovering new molecules with biological activity. The objective of this research work was to implement the use of chemometric algorithms for the identification of variations in the metabolome of the microbial antagonism between Pseudovibrio denitrificans and Vibrio harveyi. Extracts from cultured bacteria and used culture media were analyzed by Ultra-high performance liquid chromatography coupled with a diode-array detector (UHPLC-DAD). Additionally, chemometric algorithms were employed, subjecting to Principal Component  Analysis (PCA) for the  obtained  chromatograms.  Three peaks were found  that express the major variability between the individual metabolome and the metabolome from the interaction of P. denitrificans and V. harveyi. In this manner, metabolomic through  UHPLC- DAD and chemometric algorithms, showed to be a useful tool to identify the peaks responsible for differences between microbial interactions.

• Main Author: Chalén-Alvarado, B. (author)
• Other Authors: Quiroz-Moreno, C. (author), Mogollón, NGS. (author), Domínguez, C. (author), Rodríguez, J. (author)
• Format: article
• Language: spa
• Published: 2019
• Subjects: Microbial antagonism; liquid chromatography; principal component analysis; Pseudovibrio denitrificans; Vibrio harveyi; Biological Sciences; Antagonismo microbiano; cromatografía líquida; análisis de componentes principales; Ciencias Biológicas
• Online Access: https://perfiles.espoch.edu.ec/index.php/perfiles/article/view/50