Metabolome analysis in the microbial antagonism by liquid chromatography coupled with chemometrics algorithms
Metabolome is a group of low molecular weight organic compounds (metabolites) produced by biological systems. Bacterial antagonism is an important evolving force, in this sense analysis of its metabolome represents a useful tool for discovering new molecules with biological activity. The objective o...
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2019
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| Online Erişim: | https://perfiles.espoch.edu.ec/index.php/perfiles/article/view/50 |
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Etiketle | _version_ | 1845166842247643136 |
|---|---|
| author | Chalén-Alvarado, B. |
| author2 | Quiroz-Moreno, C. Mogollón, NGS. Domínguez, C. Rodríguez, J. |
| author2_role | author author author author |
| author_facet | Chalén-Alvarado, B. Quiroz-Moreno, C. Mogollón, NGS. Domínguez, C. Rodríguez, J. |
| author_role | author |
| collection | Revista Perfiles |
| dc.creator.none.fl_str_mv | Chalén-Alvarado, B. Quiroz-Moreno, C. Mogollón, NGS. Domínguez, C. Rodríguez, J. |
| dc.date.none.fl_str_mv | 2019-07-31 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://perfiles.espoch.edu.ec/index.php/perfiles/article/view/50 10.47187/perf.v2i22.50 |
| dc.language.none.fl_str_mv | spa |
| dc.publisher.none.fl_str_mv | Escuela Superior Politécnica de Chimborazo |
| dc.relation.none.fl_str_mv | https://perfiles.espoch.edu.ec/index.php/perfiles/article/view/50/28 |
| dc.rights.none.fl_str_mv | https://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | Perfiles; Vol. 2 No. 22 (2019): [July - December 2019]; 20-25 Perfiles; Vol. 2 Núm. 22 (2019): [Julio - Diciembre 2019]; 20-25 2477-9105 reponame:Revista Perfiles instname:Escuela Superior Politécnica de Chimborazo instacron:ESPOCH |
| dc.subject.none.fl_str_mv | Microbial antagonism liquid chromatography principal component analysis Pseudovibrio denitrificans Vibrio harveyi Biological Sciences Antagonismo microbiano cromatografía líquida análisis de componentes principales Pseudovibrio denitrificans Vibrio harveyi Ciencias Biológicas |
| dc.title.none.fl_str_mv | Metabolome analysis in the microbial antagonism by liquid chromatography coupled with chemometrics algorithms ESTUDIO DEL METABOLOMA EN EL ANTAGONISMO MICROBIANO A TRAVÉS DE CROMATOGRAFÍA LÍQUIDA CON ALGORITMOS QUIMIOMÉTRICOS |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo revisado por pares |
| description | Metabolome is a group of low molecular weight organic compounds (metabolites) produced by biological systems. Bacterial antagonism is an important evolving force, in this sense analysis of its metabolome represents a useful tool for discovering new molecules with biological activity. The objective of this research work was to implement the use of chemometric algorithms for the identification of variations in the metabolome of the microbial antagonism between Pseudovibrio denitrificans and Vibrio harveyi. Extracts from cultured bacteria and used culture media were analyzed by Ultra-high performance liquid chromatography coupled with a diode-array detector (UHPLC-DAD). Additionally, chemometric algorithms were employed, subjecting to Principal Component Analysis (PCA) for the obtained chromatograms. Three peaks were found that express the major variability between the individual metabolome and the metabolome from the interaction of P. denitrificans and V. harveyi. In this manner, metabolomic through UHPLC- DAD and chemometric algorithms, showed to be a useful tool to identify the peaks responsible for differences between microbial interactions. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | REVPERFIL_b037fa457b0c8f275f4e6ef33faa8ce2 |
| identifier_str_mv | 10.47187/perf.v2i22.50 |
| instacron_str | ESPOCH |
| institution | ESPOCH |
| instname_str | Escuela Superior Politécnica de Chimborazo |
| language | spa |
| network_acronym_str | REVPERFIL |
| network_name_str | Revista Perfiles |
| oai_identifier_str | oai:ojs2.localhost:article/50 |
| publishDate | 2019 |
| publisher.none.fl_str_mv | Escuela Superior Politécnica de Chimborazo |
| reponame_str | Revista Perfiles |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | Revista Perfiles - Escuela Superior Politécnica de Chimborazo |
| repository_id_str | 0 |
| rights_invalid_str_mv | https://creativecommons.org/licenses/by-nc/4.0 |
| spelling | Metabolome analysis in the microbial antagonism by liquid chromatography coupled with chemometrics algorithmsESTUDIO DEL METABOLOMA EN EL ANTAGONISMO MICROBIANO A TRAVÉS DE CROMATOGRAFÍA LÍQUIDA CON ALGORITMOS QUIMIOMÉTRICOS Chalén-Alvarado, B.Quiroz-Moreno, C.Mogollón, NGS.Domínguez, C.Rodríguez, J.Microbial antagonismliquid chromatographyprincipal component analysisPseudovibrio denitrificansVibrio harveyiBiological SciencesAntagonismo microbianocromatografía líquidaanálisis de componentes principalesPseudovibrio denitrificansVibrio harveyiCiencias BiológicasMetabolome is a group of low molecular weight organic compounds (metabolites) produced by biological systems. Bacterial antagonism is an important evolving force, in this sense analysis of its metabolome represents a useful tool for discovering new molecules with biological activity. The objective of this research work was to implement the use of chemometric algorithms for the identification of variations in the metabolome of the microbial antagonism between Pseudovibrio denitrificans and Vibrio harveyi. Extracts from cultured bacteria and used culture media were analyzed by Ultra-high performance liquid chromatography coupled with a diode-array detector (UHPLC-DAD). Additionally, chemometric algorithms were employed, subjecting to Principal Component Analysis (PCA) for the obtained chromatograms. Three peaks were found that express the major variability between the individual metabolome and the metabolome from the interaction of P. denitrificans and V. harveyi. In this manner, metabolomic through UHPLC- DAD and chemometric algorithms, showed to be a useful tool to identify the peaks responsible for differences between microbial interactions.El metaboloma es el conjunto de compuestos orgánicos de bajo peso molecular (metabolitos) pro- ducidos por sistemas biológicos. El antagonismo microbiano es una importante fuerza evolutiva, por lo que el análisis de su metaboloma es una herramienta útil para la búsqueda de nuevas molé- culas con actividad biológica. El objetivo de este trabajo fue implementar el uso de algoritmos qui- miométricos para la identificación de variaciones en el metaboloma del antagonismo microbiano entre Pseudovibrio denitrificans y Vibrio harveyi. Extractos de bacterias y del medio de cultivo usado fueron analizados por cromatografía líquida de ultra alta eficiencia acoplada a un detec- tor de arreglo de diodos (UHPLC-DAD). Además, algoritmos quimiométricos fueron aplicados realizando un Análisis de Componentes Principales (PCA) de los cromatogramas obtenidos. Se encontraron tres picos que expresaron una mayor variabilidad entre el metaboloma individual y el metaboloma de la interacción de P. denitrificans y V. harveyi. De esta manera, la metabolómica con UHPLC-DAD y algoritmos quimiométricos demostró ser una herramienta útil para la identi- ficación de picos responsables de las diferencias entre interacciones microbianas.Escuela Superior Politécnica de Chimborazo2019-07-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtículo revisado por paresapplication/pdfhttps://perfiles.espoch.edu.ec/index.php/perfiles/article/view/5010.47187/perf.v2i22.50Perfiles; Vol. 2 No. 22 (2019): [July - December 2019]; 20-25Perfiles; Vol. 2 Núm. 22 (2019): [Julio - Diciembre 2019]; 20-252477-9105reponame:Revista Perfilesinstname:Escuela Superior Politécnica de Chimborazoinstacron:ESPOCHspahttps://perfiles.espoch.edu.ec/index.php/perfiles/article/view/50/28https://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccess2024-08-24T00:49:32Zoai:ojs2.localhost:article/50Portal de revistashttps://perfiles.espoch.edu.ec/Universidad públicahttps://espoch.edu.ecEcuador2477-91051390-5740opendoar:02024-08-24T00:49:32Revista Perfiles - Escuela Superior Politécnica de Chimborazofalse |
| spellingShingle | Metabolome analysis in the microbial antagonism by liquid chromatography coupled with chemometrics algorithms Chalén-Alvarado, B. Microbial antagonism liquid chromatography principal component analysis Pseudovibrio denitrificans Vibrio harveyi Biological Sciences Antagonismo microbiano cromatografía líquida análisis de componentes principales Pseudovibrio denitrificans Vibrio harveyi Ciencias Biológicas |
| status_str | publishedVersion |
| title | Metabolome analysis in the microbial antagonism by liquid chromatography coupled with chemometrics algorithms |
| title_full | Metabolome analysis in the microbial antagonism by liquid chromatography coupled with chemometrics algorithms |
| title_fullStr | Metabolome analysis in the microbial antagonism by liquid chromatography coupled with chemometrics algorithms |
| title_full_unstemmed | Metabolome analysis in the microbial antagonism by liquid chromatography coupled with chemometrics algorithms |
| title_short | Metabolome analysis in the microbial antagonism by liquid chromatography coupled with chemometrics algorithms |
| title_sort | Metabolome analysis in the microbial antagonism by liquid chromatography coupled with chemometrics algorithms |
| topic | Microbial antagonism liquid chromatography principal component analysis Pseudovibrio denitrificans Vibrio harveyi Biological Sciences Antagonismo microbiano cromatografía líquida análisis de componentes principales Pseudovibrio denitrificans Vibrio harveyi Ciencias Biológicas |
| url | https://perfiles.espoch.edu.ec/index.php/perfiles/article/view/50 |