Effect of aqueous extract of Ocotea quixos leaf on Rhipicephalus microplus in vitro
Ticks of the Ixodidae family represent 78% of the known species, affecting 80% of cattle. The objective was to evaluate the tick-killing effect of the aqueous extract of ocotea leaves. The ocotea hydrolate was obtained by steam drag. A completely randomized design with a factorial arrangement and tw...
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| Tác giả khác: | , , , |
| Định dạng: | article |
| Ngôn ngữ: | spa eng |
| Được phát hành: |
2026
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| Những chủ đề: | |
| Truy cập trực tuyến: | https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/8162 |
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Thêm thẻ | Tóm tắt: | Ticks of the Ixodidae family represent 78% of the known species, affecting 80% of cattle. The objective was to evaluate the tick-killing effect of the aqueous extract of ocotea leaves. The ocotea hydrolate was obtained by steam drag. A completely randomized design with a factorial arrangement and two levels, hydrolate concentration (25%, 62.5% and 100%), three immersion times (1, 3.5 and 6 minutes), and two controls (a positive one with a commercial tick killer Biorbos and a negative one with distilled water) on tick mortality was used. The data were analyzed using the Design-Expert software version 12. The results were experimentally validated, reaching values very similar to those expected, through the polynomial model that presented the best fit with an R2 value of 0.9596. The interaction of the factors indicated that the effect of immersion time on mortality depends on the concentration of the hydrolate. The tick-killing effect of the aqueous extract showed a significantly high mortality with an average of 8 dead ticks per Petri dish (80% mortality), a value similar to the positive control (C+), which reached 100% mortality. The treatments with 62.5% of hydrolate at 3.5 minutes showed an intermediate mortality with an average of 7 dead ticks (70% mortality). It was concluded that the hydrolate at a concentration of 100% with an immersion time of 6 minutes had a positive effect on the mortality of Rhipicephalus microplus ticks |
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