Aislamiento y selección de bacterias con capacidad de degradar celulosa y almidón
The intention of isolating and selecting autochthonous bacterial strains with potential to degradation of cellulose and starch; five different environments were chosen (organic area, AO; conventional area, AQ; Forest, BM; sugarcane area, RC and Compost, C) which were located on plates-traps containi...
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2011
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| author | Zambrano Pazmiño, Diego Efrén |
| author2 | Guzmán Cedeño, Ángel Monserrate Rondón Castillo, Ana Julia |
| author2_role | author author |
| author_facet | Zambrano Pazmiño, Diego Efrén Guzmán Cedeño, Ángel Monserrate Rondón Castillo, Ana Julia |
| author_role | author |
| collection | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| dc.creator.none.fl_str_mv | Zambrano Pazmiño, Diego Efrén Guzmán Cedeño, Ángel Monserrate Rondón Castillo, Ana Julia |
| dc.date.none.fl_str_mv | 2011-12-21 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/173 |
| dc.language.none.fl_str_mv | spa |
| dc.publisher.none.fl_str_mv | Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López |
| dc.relation.none.fl_str_mv | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/173/173 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | Revista ESPAMCIENCIA; Vol. 2 Núm. 2 (2011): REVISTA ESPAMCIENCIA 2011; 61-68 1390-8103 reponame:Revistas Escuela Superior Politécnica Agropecuaria de Manabí instname:Escuela Superior Politécnica Agropecuaria de Manabí instacron:ESPAM |
| dc.subject.none.fl_str_mv | bacterias enzimas celulolíticas CMC almidón degradación Bacteria cellulolytic enzymes CMC starch degradation |
| dc.title.none.fl_str_mv | Aislamiento y selección de bacterias con capacidad de degradar celulosa y almidón |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | The intention of isolating and selecting autochthonous bacterial strains with potential to degradation of cellulose and starch; five different environments were chosen (organic area, AO; conventional area, AQ; Forest, BM; sugarcane area, RC and Compost, C) which were located on plates-traps containing modified nutrient agar, and soil samples taken from those sites. Plates-traps were collected at 24 hours and taken to laboratory for sampling of visible colonies. Soil samples were serially diluted. In both cases, it was plated by exhaustion and purifying to obtain a strain collection of 93 strains (71 from soil samples and 22 traps). All agar seeded in carboxy methyl cellulose (CMC) to determine the cellulolytic activity by revealing them with congo red from the hydrolysis halo. Amylolytic activity was assessed by placing the strains in an environment whose only energy source was starch as revealed with Lugol’s iodine solution. AO-19 strain isolated from the organic area made the largest halo cellulose degradation 12.33 IU/mm while BM-1 strain isolated from the forest obtained 9.33 IU/mm diameter of starch hydrolysis halo. AO-19 strain has potential to be considered in obtaining bacterial bio preparations or composting organic fibrous material. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | RevESPAM_135f72f7a1a968ad9f1c6458923ddaf1 |
| instacron_str | ESPAM |
| institution | ESPAM |
| instname_str | Escuela Superior Politécnica Agropecuaria de Manabí |
| language | spa |
| network_acronym_str | RevESPAM |
| network_name_str | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| oai_identifier_str | oai:ojs.pkp.sfu.ca:article/173 |
| publishDate | 2011 |
| publisher.none.fl_str_mv | Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López |
| reponame_str | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Revistas Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabí |
| repository_id_str | 0 |
| spelling | Aislamiento y selección de bacterias con capacidad de degradar celulosa y almidónZambrano Pazmiño, Diego EfrénGuzmán Cedeño, Ángel MonserrateRondón Castillo, Ana Juliabacteriasenzimas celulolíticasCMCalmidóndegradaciónBacteriacellulolytic enzymesCMCstarchdegradation The intention of isolating and selecting autochthonous bacterial strains with potential to degradation of cellulose and starch; five different environments were chosen (organic area, AO; conventional area, AQ; Forest, BM; sugarcane area, RC and Compost, C) which were located on plates-traps containing modified nutrient agar, and soil samples taken from those sites. Plates-traps were collected at 24 hours and taken to laboratory for sampling of visible colonies. Soil samples were serially diluted. In both cases, it was plated by exhaustion and purifying to obtain a strain collection of 93 strains (71 from soil samples and 22 traps). All agar seeded in carboxy methyl cellulose (CMC) to determine the cellulolytic activity by revealing them with congo red from the hydrolysis halo. Amylolytic activity was assessed by placing the strains in an environment whose only energy source was starch as revealed with Lugol’s iodine solution. AO-19 strain isolated from the organic area made the largest halo cellulose degradation 12.33 IU/mm while BM-1 strain isolated from the forest obtained 9.33 IU/mm diameter of starch hydrolysis halo. AO-19 strain has potential to be considered in obtaining bacterial bio preparations or composting organic fibrous material. Con la intención de aislar y seleccionar cepas bacterianas autóctonas con potencial para la degradación de celulosa y almidón se escogieron cinco ambientes (área orgánica, A.O; área convencional, A.Q; Bosque, B.M; área cañera, R.C y Compost, C) donde se ubicaron placas-trampas que contenían agar nutriente modificado, además se tomaron muestras de suelo de esos sitios. Las placas-trampas fueron recogidas a las 24 horas y llevadas a laboratorio para extraer muestras de las colonias visibles. Con las muestras de suelo se realizaron diluciones seriadas. En ambos casos, se sembró en placas y se purificó por agotamiento hasta obtener un cepario de 93 cepas (71 provenientes de muestras de suelo y 22 de trampas). Todas se sembraron en agar Carboximetilcelulosa (CMC) para determinar la actividad celulolítica mediante revelado con rojo congo, del halo de hidrólisis. La actividad amilolítica se evaluó sembrando las cepas en un medio cuya única fuente energética era el almidón y se reveló con solución yodo-lugol. La cepa A.O-19 aislada del área orgánica efectuó el mayor halo de degradación de celulosa con 12.33 UI/mm mientras que la cepa B.M-1, Aislada del bosque obtuvo 9.33 UI/mm de diámetro de halo de hidrólisis del almidón. La cepa A.O-19 tiene potencial para ser considerada en la obtención de biopreparados bacterianos u inoculos en compostaje de material orgánico fibroso Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López2011-12-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/173Revista ESPAMCIENCIA; Vol. 2 Núm. 2 (2011): REVISTA ESPAMCIENCIA 2011; 61-681390-8103reponame:Revistas Escuela Superior Politécnica Agropecuaria de Manabíinstname:Escuela Superior Politécnica Agropecuaria de Manabíinstacron:ESPAMspahttps://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/173/173info:eu-repo/semantics/openAccess2025-02-25T14:25:55Zoai:ojs.pkp.sfu.ca:article/173Institucionalhttp://revistasespam.espam.edu.ec/Universidad públicahttp://www.espam.edu.ec/http://revistasespam.espam.edu.ec/oai.Ecuador.1390-81031390-597Xopendoar:02025-02-25T14:25:55Revistas Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabífalse |
| spellingShingle | Aislamiento y selección de bacterias con capacidad de degradar celulosa y almidón Zambrano Pazmiño, Diego Efrén bacterias enzimas celulolíticas CMC almidón degradación Bacteria cellulolytic enzymes CMC starch degradation |
| status_str | publishedVersion |
| title | Aislamiento y selección de bacterias con capacidad de degradar celulosa y almidón |
| title_full | Aislamiento y selección de bacterias con capacidad de degradar celulosa y almidón |
| title_fullStr | Aislamiento y selección de bacterias con capacidad de degradar celulosa y almidón |
| title_full_unstemmed | Aislamiento y selección de bacterias con capacidad de degradar celulosa y almidón |
| title_short | Aislamiento y selección de bacterias con capacidad de degradar celulosa y almidón |
| title_sort | Aislamiento y selección de bacterias con capacidad de degradar celulosa y almidón |
| topic | bacterias enzimas celulolíticas CMC almidón degradación Bacteria cellulolytic enzymes CMC starch degradation |
| url | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/173 |