Levels of ochratoxin a produced by Aspergillus in arabica coffee in Southern Manabí
The objective of this research was to conduct a post-harvest level study on coffee processed by the dry method, aiming to determine contamination levels of Ochratoxin A (OTA) in Arabica coffee (Coffea arabica L.) in the southern region of Manabí province, using the high-performance liquid chromatogr...
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2024
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| Truy cập trực tuyến: | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/560 |
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Thêm thẻ | _version_ | 1836230918625296384 |
|---|---|
| author | Soledispa Plúa, Maritza Leonor |
| author2 | García Pico, Anthony Daniel Alcívar Murillo , Rubén Melquiades Alcívar Santana, Rubén Andrés |
| author2_role | author author author |
| author_facet | Soledispa Plúa, Maritza Leonor García Pico, Anthony Daniel Alcívar Murillo , Rubén Melquiades Alcívar Santana, Rubén Andrés |
| author_role | author |
| collection | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| dc.creator.none.fl_str_mv | Soledispa Plúa, Maritza Leonor García Pico, Anthony Daniel Alcívar Murillo , Rubén Melquiades Alcívar Santana, Rubén Andrés |
| dc.date.none.fl_str_mv | 2024-11-30 |
| dc.format.none.fl_str_mv | application/pdf text/html application/zip |
| dc.identifier.none.fl_str_mv | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/560 10.51260/1390597X.560 |
| dc.language.none.fl_str_mv | spa |
| dc.publisher.none.fl_str_mv | Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López |
| dc.relation.none.fl_str_mv | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/560/962 https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/560/963 https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/560/964 |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | Revista ESPAMCIENCIA; Vol. 15 Núm. E (2024): REVISTA ESPAMCIENCIA 2024 1390-8103 reponame:Revistas Escuela Superior Politécnica Agropecuaria de Manabí instname:Escuela Superior Politécnica Agropecuaria de Manabí instacron:ESPAM |
| dc.subject.none.fl_str_mv | Micotoxinas Ocratoxina A (OTA) café natural cromatografía Mycotoxins Ochratoxin A (OTA) natural coffee chromatography |
| dc.title.none.fl_str_mv | Levels of ochratoxin a produced by Aspergillus in arabica coffee in Southern Manabí Niveles de ocratoxina a producida por Aspergillus en café arábigo en el Sur de Manabí |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | The objective of this research was to conduct a post-harvest level study on coffee processed by the dry method, aiming to determine contamination levels of Ochratoxin A (OTA) in Arabica coffee (Coffea arabica L.) in the southern region of Manabí province, using the high-performance liquid chromatography technique (HPLC-Fluorescence). A total of 9 samples of natural coffee were analyzed, collected from producers and traders in the cantons of Jipijapa, Paján, and 24 de Mayo, located in the southern region of Manabí. These were microbiologically analyzed using the PEE-LSAIA-01 test method to quantify levels of Ochratoxin A (OTA). The detection limit for Ochratoxin A was 0.018 ng/mL, corresponding to a concentration of 0.86 μg/kg in the sample. All nine samples (100%) tested positive for Ochratoxin A. The levels detected ranged from 30.63 to 445.60 μg/kg; all were above the European Union (EU) permitted limit for OTA concentrations of 5.0 μg/kg. The analysis revealed that the management and processing of coffee produced in the region do not meet international post-harvest handling and storage standards, resulting in a high incidence and very high levels of mycotoxin-producing fungi. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | RevESPAM_25d8b2fbfb0e376a96b764a21887cb2d |
| identifier_str_mv | 10.51260/1390597X.560 |
| instacron_str | ESPAM |
| institution | ESPAM |
| instname_str | Escuela Superior Politécnica Agropecuaria de Manabí |
| language | spa |
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| network_name_str | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| oai_identifier_str | oai:ojs.pkp.sfu.ca:article/560 |
| publishDate | 2024 |
| publisher.none.fl_str_mv | Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López |
| reponame_str | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Revistas Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabí |
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| spelling | Levels of ochratoxin a produced by Aspergillus in arabica coffee in Southern ManabíNiveles de ocratoxina a producida por Aspergillus en café arábigo en el Sur de ManabíSoledispa Plúa, Maritza LeonorGarcía Pico, Anthony DanielAlcívar Murillo , Rubén MelquiadesAlcívar Santana, Rubén AndrésMicotoxinasOcratoxina A (OTA)café naturalcromatografíaMycotoxinsOchratoxin A (OTA)natural coffeechromatographyThe objective of this research was to conduct a post-harvest level study on coffee processed by the dry method, aiming to determine contamination levels of Ochratoxin A (OTA) in Arabica coffee (Coffea arabica L.) in the southern region of Manabí province, using the high-performance liquid chromatography technique (HPLC-Fluorescence). A total of 9 samples of natural coffee were analyzed, collected from producers and traders in the cantons of Jipijapa, Paján, and 24 de Mayo, located in the southern region of Manabí. These were microbiologically analyzed using the PEE-LSAIA-01 test method to quantify levels of Ochratoxin A (OTA). The detection limit for Ochratoxin A was 0.018 ng/mL, corresponding to a concentration of 0.86 μg/kg in the sample. All nine samples (100%) tested positive for Ochratoxin A. The levels detected ranged from 30.63 to 445.60 μg/kg; all were above the European Union (EU) permitted limit for OTA concentrations of 5.0 μg/kg. The analysis revealed that the management and processing of coffee produced in the region do not meet international post-harvest handling and storage standards, resulting in a high incidence and very high levels of mycotoxin-producing fungi.La investigación tuvo como objetivo realizar un estudio a nivel de poscosecha en café beneficiado por el proceso de vía seca, con el objetivo de determinar los niveles de contaminación por Ocratoxina A (OTA) en café arábigo (Coffea arábiga L.), en la región sur de la provincia Manabí, por medio de la técnica de cromatografía líquida de alta resolución (HPLC-Fluorescencia). Se analizaron 9 muestras de café natural, de productores y comerciantes de los cantones de Jipijapa, Paján y 24 de Mayo ubicados en la región sur de Manabí. Fueron analizados microbiológicamente por el método de ensayo PEE-LSAIA-01 para cuantificar los niveles de Ocratoxina A (OTA). El límite de detección de ocratoxina A fue de 0,018 ng/mL, correspondiente a una concentración de 0,86 μg/kg en la muestra. De las nueve muestras (100%) resultaron positivas por Ocratoxina A. Los niveles presentados oscilaron entre 30,63 y 445,60 μg/kg; todos estaban por encima del límite permitido por la Unión Europea UE para concentraciones de OTA de 5,0 μg/kg. El análisis reveló que el manejo y procesamiento del café producido en la región no cumple con los estándares internacionales para el manejo y almacenamiento poscosecha, lo que resultó en una alta incidencia y niveles muy altos de hongos productores de micotoxinas.Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López2024-11-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlapplication/ziphttps://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/56010.51260/1390597X.560Revista ESPAMCIENCIA; Vol. 15 Núm. E (2024): REVISTA ESPAMCIENCIA 20241390-8103reponame:Revistas Escuela Superior Politécnica Agropecuaria de Manabíinstname:Escuela Superior Politécnica Agropecuaria de Manabíinstacron:ESPAMspahttps://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/560/962https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/560/963https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/560/964http://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccess2025-06-19T19:21:22Zoai:ojs.pkp.sfu.ca:article/560Institucionalhttp://revistasespam.espam.edu.ec/Universidad públicahttp://www.espam.edu.ec/http://revistasespam.espam.edu.ec/oai.Ecuador.1390-81031390-597Xopendoar:02025-06-19T19:21:22Revistas Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabífalse |
| spellingShingle | Levels of ochratoxin a produced by Aspergillus in arabica coffee in Southern Manabí Soledispa Plúa, Maritza Leonor Micotoxinas Ocratoxina A (OTA) café natural cromatografía Mycotoxins Ochratoxin A (OTA) natural coffee chromatography |
| status_str | publishedVersion |
| title | Levels of ochratoxin a produced by Aspergillus in arabica coffee in Southern Manabí |
| title_full | Levels of ochratoxin a produced by Aspergillus in arabica coffee in Southern Manabí |
| title_fullStr | Levels of ochratoxin a produced by Aspergillus in arabica coffee in Southern Manabí |
| title_full_unstemmed | Levels of ochratoxin a produced by Aspergillus in arabica coffee in Southern Manabí |
| title_short | Levels of ochratoxin a produced by Aspergillus in arabica coffee in Southern Manabí |
| title_sort | Levels of ochratoxin a produced by Aspergillus in arabica coffee in Southern Manabí |
| topic | Micotoxinas Ocratoxina A (OTA) café natural cromatografía Mycotoxins Ochratoxin A (OTA) natural coffee chromatography |
| url | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/560 |