Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae)
A The objective was to isolate bacteria, yeasts and an ascomycete fungus from the digestive fluid of Rhynchophorus palmarum larvae. The larvae were acquired in typical product markets of the Amazon. The specimens were selected according to their approximate weight and size, to later be dissected und...
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| Other Authors: | , , , |
| Format: | article |
| Language: | spa |
| Published: |
2021
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| Online Access: | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251 |
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Add Tag | Summary: | A The objective was to isolate bacteria, yeasts and an ascomycete fungus from the digestive fluid of Rhynchophorus palmarum larvae. The larvae were acquired in typical product markets of the Amazon. The specimens were selected according to their approximate weight and size, to later be dissected under sterile conditions and obtain a suspension of intestinal fluid, which was diluted and sown in solid and liquid medium. For microbial isolation, approaches were considered for the culture of bacteria and fungi or yeasts, both under aerobic, anaerobic and microaerophilic conditions. The isolated colonies were brought up to pure cultures, obtaining a total of fourteen isolates, mostly represented by Gram negative bacilli and, to a lesser extent, by fungi and yeasts. Regarding their oxygen requirement, most of the isolates correspond to aerobic microorganisms, with a scarce presence of anaerobic and microaerophilic isolates. |
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