Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae)
A The objective was to isolate bacteria, yeasts and an ascomycete fungus from the digestive fluid of Rhynchophorus palmarum larvae. The larvae were acquired in typical product markets of the Amazon. The specimens were selected according to their approximate weight and size, to later be dissected und...
Պահպանված է:
Հիմնական հեղինակ: | |
---|---|
Այլ հեղինակներ: | , , , |
Ձևաչափ: | article |
Լեզու: | spa |
Հրապարակվել է: |
2021
|
Խորագրեր: | |
Առցանց հասանելիություն: | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251 |
Ցուցիչներ: |
Ավելացրեք ցուցիչ
Չկան պիտակներ, Եղեք առաջինը, ով նշում է այս գրառումը!
|
_version_ | 1837499036050915328 |
---|---|
author | Landivar, Marcos David |
author2 | Sancho Aguilera, David Pico Poma, Josselyn Paulina Sarabia Guevara, Diego Abelardo Pintado Vallejo, Pamela Jacqueline |
author2_role | author author author author |
author_facet | Landivar, Marcos David Sancho Aguilera, David Pico Poma, Josselyn Paulina Sarabia Guevara, Diego Abelardo Pintado Vallejo, Pamela Jacqueline |
author_role | author |
collection | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
dc.creator.none.fl_str_mv | Landivar, Marcos David Sancho Aguilera, David Pico Poma, Josselyn Paulina Sarabia Guevara, Diego Abelardo Pintado Vallejo, Pamela Jacqueline |
dc.date.none.fl_str_mv | 2021-12-30 |
dc.format.none.fl_str_mv | application/pdf text/html application/zip |
dc.identifier.none.fl_str_mv | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251 10.51260/revista_espamciencia.v12i2.251 |
dc.language.none.fl_str_mv | spa |
dc.publisher.none.fl_str_mv | Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López |
dc.relation.none.fl_str_mv | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251/288 https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251/399 https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251/558 |
dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
dc.source.none.fl_str_mv | Revista ESPAMCIENCIA; Vol. 12 Núm. 2 (2021): REVISTA ESPAMCIENCIA 2021; 152-157 1390-8103 reponame:Revistas Escuela Superior Politécnica Agropecuaria de Manabí instname:Escuela Superior Politécnica Agropecuaria de Manabí instacron:ESPAM |
dc.subject.none.fl_str_mv | Larvas aislamiento microorganismos Rhynchophorus palmarum Larvae isolation microorganisms Rhynchophorus palmarum |
dc.title.none.fl_str_mv | Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae) Aislamiento de microorganismos a partir del fluido digestivo de larvas de Rhynchophorus palmarum (Coleoptera: Curculionidae) |
dc.type.none.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
description | A The objective was to isolate bacteria, yeasts and an ascomycete fungus from the digestive fluid of Rhynchophorus palmarum larvae. The larvae were acquired in typical product markets of the Amazon. The specimens were selected according to their approximate weight and size, to later be dissected under sterile conditions and obtain a suspension of intestinal fluid, which was diluted and sown in solid and liquid medium. For microbial isolation, approaches were considered for the culture of bacteria and fungi or yeasts, both under aerobic, anaerobic and microaerophilic conditions. The isolated colonies were brought up to pure cultures, obtaining a total of fourteen isolates, mostly represented by Gram negative bacilli and, to a lesser extent, by fungi and yeasts. Regarding their oxygen requirement, most of the isolates correspond to aerobic microorganisms, with a scarce presence of anaerobic and microaerophilic isolates. |
eu_rights_str_mv | openAccess |
format | article |
id | RevESPAM_9238418f990793b9d636e97d53aeec31 |
identifier_str_mv | 10.51260/revista_espamciencia.v12i2.251 |
instacron_str | ESPAM |
institution | ESPAM |
instname_str | Escuela Superior Politécnica Agropecuaria de Manabí |
language | spa |
network_acronym_str | RevESPAM |
network_name_str | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
oai_identifier_str | oai:ojs.pkp.sfu.ca:article/251 |
publishDate | 2021 |
publisher.none.fl_str_mv | Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López |
reponame_str | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
repository.mail.fl_str_mv | . |
repository.name.fl_str_mv | Revistas Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabí |
repository_id_str | 0 |
spelling | Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae)Aislamiento de microorganismos a partir del fluido digestivo de larvas de Rhynchophorus palmarum (Coleoptera: Curculionidae)Landivar, Marcos DavidSancho Aguilera, DavidPico Poma, Josselyn PaulinaSarabia Guevara, Diego AbelardoPintado Vallejo, Pamela JacquelineLarvasaislamientomicroorganismosRhynchophoruspalmarumLarvaeisolationmicroorganismsRhynchophoruspalmarumA The objective was to isolate bacteria, yeasts and an ascomycete fungus from the digestive fluid of Rhynchophorus palmarum larvae. The larvae were acquired in typical product markets of the Amazon. The specimens were selected according to their approximate weight and size, to later be dissected under sterile conditions and obtain a suspension of intestinal fluid, which was diluted and sown in solid and liquid medium. For microbial isolation, approaches were considered for the culture of bacteria and fungi or yeasts, both under aerobic, anaerobic and microaerophilic conditions. The isolated colonies were brought up to pure cultures, obtaining a total of fourteen isolates, mostly represented by Gram negative bacilli and, to a lesser extent, by fungi and yeasts. Regarding their oxygen requirement, most of the isolates correspond to aerobic microorganisms, with a scarce presence of anaerobic and microaerophilic isolates.Se tuvo como objetivo aislar bacterias, levaduras y un hongo ascomiceto, a partir del fluido digestivo de larvas de Rhynchophorus palmarum. Las larvas fueron adquiridas en mercados de productos típicos de la Amazonia. Los ejemplares se seleccionaron de acuerdo a su peso y talla aproximados, para luego ser disecados en condiciones de esterilidad y obtener una suspensión de fluido intestinal, que fue diluida y sembrada en medio sólido y líquido. Para el aislamiento microbiano, se consideraron aproximaciones para el cultivo de bacterias y hongos o levaduras, tanto en condiciones aeróbicas como anaeróbicas y microaerófilas. Las colonias aisladas se llevaron hasta cultivos puros, obteniéndose un total de catorce aislados, representados en su mayoría por bacilos Gram negativos y, en menor proporción por hongos y levaduras. En cuanto a su requerimiento de oxígeno, la mayoría de los aislados corresponde a microorganismos aeróbicos, con una escasa presencia de aislados anaeróbicos y microaerófilos.Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López2021-12-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlapplication/ziphttps://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/25110.51260/revista_espamciencia.v12i2.251Revista ESPAMCIENCIA; Vol. 12 Núm. 2 (2021): REVISTA ESPAMCIENCIA 2021; 152-1571390-8103reponame:Revistas Escuela Superior Politécnica Agropecuaria de Manabíinstname:Escuela Superior Politécnica Agropecuaria de Manabíinstacron:ESPAMspahttps://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251/288https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251/399https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251/558info:eu-repo/semantics/openAccess2025-02-25T14:40:11Zoai:ojs.pkp.sfu.ca:article/251Institucionalhttp://revistasespam.espam.edu.ec/Universidad públicahttp://www.espam.edu.ec/http://revistasespam.espam.edu.ec/oai.Ecuador.1390-81031390-597Xopendoar:02025-02-25T14:40:11Revistas Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabífalse |
spellingShingle | Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae) Landivar, Marcos David Larvas aislamiento microorganismos Rhynchophorus palmarum Larvae isolation microorganisms Rhynchophorus palmarum |
status_str | publishedVersion |
title | Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae) |
title_full | Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae) |
title_fullStr | Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae) |
title_full_unstemmed | Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae) |
title_short | Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae) |
title_sort | Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae) |
topic | Larvas aislamiento microorganismos Rhynchophorus palmarum Larvae isolation microorganisms Rhynchophorus palmarum |
url | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251 |