Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae)

A The objective was to isolate bacteria, yeasts and an ascomycete fungus from the digestive fluid of Rhynchophorus palmarum larvae. The larvae were acquired in typical product markets of the Amazon. The specimens were selected according to their approximate weight and size, to later be dissected und...

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Հիմնական հեղինակ: Landivar, Marcos David (author)
Այլ հեղինակներ: Sancho Aguilera, David (author), Pico Poma, Josselyn Paulina (author), Sarabia Guevara, Diego Abelardo (author), Pintado Vallejo, Pamela Jacqueline (author)
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Լեզու:spa
Հրապարակվել է: 2021
Խորագրեր:
Առցանց հասանելիություն:https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251
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author Landivar, Marcos David
author2 Sancho Aguilera, David
Pico Poma, Josselyn Paulina
Sarabia Guevara, Diego Abelardo
Pintado Vallejo, Pamela Jacqueline
author2_role author
author
author
author
author_facet Landivar, Marcos David
Sancho Aguilera, David
Pico Poma, Josselyn Paulina
Sarabia Guevara, Diego Abelardo
Pintado Vallejo, Pamela Jacqueline
author_role author
collection Revistas Escuela Superior Politécnica Agropecuaria de Manabí
dc.creator.none.fl_str_mv Landivar, Marcos David
Sancho Aguilera, David
Pico Poma, Josselyn Paulina
Sarabia Guevara, Diego Abelardo
Pintado Vallejo, Pamela Jacqueline
dc.date.none.fl_str_mv 2021-12-30
dc.format.none.fl_str_mv application/pdf
text/html
application/zip
dc.identifier.none.fl_str_mv https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251
10.51260/revista_espamciencia.v12i2.251
dc.language.none.fl_str_mv spa
dc.publisher.none.fl_str_mv Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López
dc.relation.none.fl_str_mv https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251/288
https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251/399
https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251/558
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.source.none.fl_str_mv Revista ESPAMCIENCIA; Vol. 12 Núm. 2 (2021): REVISTA ESPAMCIENCIA 2021; 152-157
1390-8103
reponame:Revistas Escuela Superior Politécnica Agropecuaria de Manabí
instname:Escuela Superior Politécnica Agropecuaria de Manabí
instacron:ESPAM
dc.subject.none.fl_str_mv Larvas
aislamiento
microorganismos
Rhynchophorus
palmarum
Larvae
isolation
microorganisms
Rhynchophorus
palmarum
dc.title.none.fl_str_mv Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae)
Aislamiento de microorganismos a partir del fluido digestivo de larvas de Rhynchophorus palmarum (Coleoptera: Curculionidae)
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
description A The objective was to isolate bacteria, yeasts and an ascomycete fungus from the digestive fluid of Rhynchophorus palmarum larvae. The larvae were acquired in typical product markets of the Amazon. The specimens were selected according to their approximate weight and size, to later be dissected under sterile conditions and obtain a suspension of intestinal fluid, which was diluted and sown in solid and liquid medium. For microbial isolation, approaches were considered for the culture of bacteria and fungi or yeasts, both under aerobic, anaerobic and microaerophilic conditions. The isolated colonies were brought up to pure cultures, obtaining a total of fourteen isolates, mostly represented by Gram negative bacilli and, to a lesser extent, by fungi and yeasts. Regarding their oxygen requirement, most of the isolates correspond to aerobic microorganisms, with a scarce presence of anaerobic and microaerophilic isolates.
eu_rights_str_mv openAccess
format article
id RevESPAM_9238418f990793b9d636e97d53aeec31
identifier_str_mv 10.51260/revista_espamciencia.v12i2.251
instacron_str ESPAM
institution ESPAM
instname_str Escuela Superior Politécnica Agropecuaria de Manabí
language spa
network_acronym_str RevESPAM
network_name_str Revistas Escuela Superior Politécnica Agropecuaria de Manabí
oai_identifier_str oai:ojs.pkp.sfu.ca:article/251
publishDate 2021
publisher.none.fl_str_mv Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López
reponame_str Revistas Escuela Superior Politécnica Agropecuaria de Manabí
repository.mail.fl_str_mv .
repository.name.fl_str_mv Revistas Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabí
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spelling Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae)Aislamiento de microorganismos a partir del fluido digestivo de larvas de Rhynchophorus palmarum (Coleoptera: Curculionidae)Landivar, Marcos DavidSancho Aguilera, DavidPico Poma, Josselyn PaulinaSarabia Guevara, Diego AbelardoPintado Vallejo, Pamela JacquelineLarvasaislamientomicroorganismosRhynchophoruspalmarumLarvaeisolationmicroorganismsRhynchophoruspalmarumA The objective was to isolate bacteria, yeasts and an ascomycete fungus from the digestive fluid of Rhynchophorus palmarum larvae. The larvae were acquired in typical product markets of the Amazon. The specimens were selected according to their approximate weight and size, to later be dissected under sterile conditions and obtain a suspension of intestinal fluid, which was diluted and sown in solid and liquid medium. For microbial isolation, approaches were considered for the culture of bacteria and fungi or yeasts, both under aerobic, anaerobic and microaerophilic conditions. The isolated colonies were brought up to pure cultures, obtaining a total of fourteen isolates, mostly represented by Gram negative bacilli and, to a lesser extent, by fungi and yeasts. Regarding their oxygen requirement, most of the isolates correspond to aerobic microorganisms, with a scarce presence of anaerobic and microaerophilic isolates.Se tuvo como objetivo aislar bacterias, levaduras y un hongo ascomiceto, a partir del fluido digestivo de larvas de Rhynchophorus palmarum. Las larvas fueron adquiridas en mercados de productos típicos de la Amazonia. Los ejemplares se seleccionaron de acuerdo a su peso y talla aproximados, para luego ser disecados en condiciones de esterilidad y obtener una suspensión de fluido intestinal, que fue diluida y sembrada en medio sólido y líquido. Para el aislamiento microbiano, se consideraron aproximaciones para el cultivo de bacterias y hongos o levaduras, tanto en condiciones aeróbicas como anaeróbicas y microaerófilas. Las colonias aisladas se llevaron hasta cultivos puros, obteniéndose un total de catorce aislados, representados en su mayoría por bacilos Gram negativos y, en menor proporción por hongos y levaduras. En cuanto a su requerimiento de oxígeno, la mayoría de los aislados corresponde a microorganismos aeróbicos, con una escasa presencia de aislados anaeróbicos y microaerófilos.Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López2021-12-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlapplication/ziphttps://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/25110.51260/revista_espamciencia.v12i2.251Revista ESPAMCIENCIA; Vol. 12 Núm. 2 (2021): REVISTA ESPAMCIENCIA 2021; 152-1571390-8103reponame:Revistas Escuela Superior Politécnica Agropecuaria de Manabíinstname:Escuela Superior Politécnica Agropecuaria de Manabíinstacron:ESPAMspahttps://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251/288https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251/399https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251/558info:eu-repo/semantics/openAccess2025-02-25T14:40:11Zoai:ojs.pkp.sfu.ca:article/251Institucionalhttp://revistasespam.espam.edu.ec/Universidad públicahttp://www.espam.edu.ec/http://revistasespam.espam.edu.ec/oai.Ecuador.1390-81031390-597Xopendoar:02025-02-25T14:40:11Revistas Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabífalse
spellingShingle Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae)
Landivar, Marcos David
Larvas
aislamiento
microorganismos
Rhynchophorus
palmarum
Larvae
isolation
microorganisms
Rhynchophorus
palmarum
status_str publishedVersion
title Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae)
title_full Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae)
title_fullStr Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae)
title_full_unstemmed Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae)
title_short Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae)
title_sort Isolation of microorganisms from the digestive fluid of larvae of Rhynchophorus palmarum (Coleoptera: Curculionidae)
topic Larvas
aislamiento
microorganismos
Rhynchophorus
palmarum
Larvae
isolation
microorganisms
Rhynchophorus
palmarum
url https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/251