Microbial diversity present in wastewater from the tuna industry
A study was carried out to identify the microbial diversity in wastewater from the tuna industry, the samples were obtained at different points in the tuna process, the analysis was carried out using molecular tools such as metagenomics, the Electrophoresis technique in Denaturation Gradient Gel (DG...
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| פורמט: | article |
| שפה: | spa |
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2022
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| גישה מקוונת: | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/312 |
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הוספת תג
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הוספת תג | _version_ | 1858426949029855232 |
|---|---|
| author | Pincay Cantos, María Fernanda |
| author_facet | Pincay Cantos, María Fernanda |
| author_role | author |
| collection | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| dc.creator.none.fl_str_mv | Pincay Cantos, María Fernanda |
| dc.date.none.fl_str_mv | 2022-06-30 |
| dc.format.none.fl_str_mv | application/pdf text/html application/zip |
| dc.identifier.none.fl_str_mv | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/312 10.51260/revista_espamciencia.v13i1.312 |
| dc.language.none.fl_str_mv | spa |
| dc.publisher.none.fl_str_mv | Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López |
| dc.relation.none.fl_str_mv | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/312/309 https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/312/394 https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/312/549 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | Revista ESPAMCIENCIA; Vol. 13 Núm. 1 (2022): REVISTA ESPAMCIENCIA 2022; 70-76 1390-8103 reponame:Revistas Escuela Superior Politécnica Agropecuaria de Manabí instname:Escuela Superior Politécnica Agropecuaria de Manabí instacron:ESPAM |
| dc.subject.none.fl_str_mv | ambiente β-galactosidasa caracterización contaminantes environment β-galactosidase characterization contaminants |
| dc.title.none.fl_str_mv | Microbial diversity present in wastewater from the tuna industry Diversidad microbiana presente en agua residual proveniente de industria atunera |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | A study was carried out to identify the microbial diversity in wastewater from the tuna industry, the samples were obtained at different points in the tuna process, the analysis was carried out using molecular tools such as metagenomics, the Electrophoresis technique in Denaturation Gradient Gel (DGGE) and Polymerase Chain Reaction (PCR). The sensitivity of these techniques allowed the amplification of 16S rDNA gene fragments by means of specific primers. This amplified PCR product was sent for sequencing, becoming a valuable alternative to provide information on the identity of the microbial population, characterizing the structure of an environment, obtaining 37 bacterial clones with 27.0% belonging to the Firmicutes group, 24.34% to Proteobacteria, 16, 21% to Actinobacteria. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | RevESPAM_a04a75a5a005aeb843ccba9739c2175a |
| identifier_str_mv | 10.51260/revista_espamciencia.v13i1.312 |
| instacron_str | ESPAM |
| institution | ESPAM |
| instname_str | Escuela Superior Politécnica Agropecuaria de Manabí |
| language | spa |
| network_acronym_str | RevESPAM |
| network_name_str | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| oai_identifier_str | oai:ojs.pkp.sfu.ca:article/312 |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López |
| reponame_str | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Revistas Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabí |
| repository_id_str | 0 |
| spelling | Microbial diversity present in wastewater from the tuna industryDiversidad microbiana presente en agua residual proveniente de industria atuneraPincay Cantos, María Fernandaambienteβ-galactosidasacaracterizacióncontaminantesenvironmentβ-galactosidasecharacterizationcontaminantsA study was carried out to identify the microbial diversity in wastewater from the tuna industry, the samples were obtained at different points in the tuna process, the analysis was carried out using molecular tools such as metagenomics, the Electrophoresis technique in Denaturation Gradient Gel (DGGE) and Polymerase Chain Reaction (PCR). The sensitivity of these techniques allowed the amplification of 16S rDNA gene fragments by means of specific primers. This amplified PCR product was sent for sequencing, becoming a valuable alternative to provide information on the identity of the microbial population, characterizing the structure of an environment, obtaining 37 bacterial clones with 27.0% belonging to the Firmicutes group, 24.34% to Proteobacteria, 16, 21% to Actinobacteria.Se realizó un estudio para identificar la diversidad microbiana en aguas residuales proveniente de la industria atunera, las muestras se obtuvieron en diferentes puntos del proceso del atún, el análisis se lo realizó por medio de herramientas moleculares como es la metagenómica, la técnica de Electroforesis en Gel con Gradiente de Desnaturalización (DGGE) y de la Reacción en cadena de la polimerasa (PCR), la sensibilidad de estas técnicas permitió la amplificación de fragmentos del gen 16S rDNA por medio de cebadores específicos este producto de PCR amplificado se envió a secuenciar, siendo la secuenciación una alternativa valiosa para proporcionar información sobre la identidad de la población microbiana, logrando caracterizar la estructura de un ambiente, obteniendo 37 clones bacterianos con un 27,0 % perteneciente al grupo Firmicutes, 24,34% a las Proteobacterias, 16,21 % a las Actinobacterias.Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López2022-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlapplication/ziphttps://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/31210.51260/revista_espamciencia.v13i1.312Revista ESPAMCIENCIA; Vol. 13 Núm. 1 (2022): REVISTA ESPAMCIENCIA 2022; 70-761390-8103reponame:Revistas Escuela Superior Politécnica Agropecuaria de Manabíinstname:Escuela Superior Politécnica Agropecuaria de Manabíinstacron:ESPAMspahttps://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/312/309https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/312/394https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/312/549info:eu-repo/semantics/openAccess2025-02-25T14:38:20Zoai:ojs.pkp.sfu.ca:article/312Institucionalhttp://revistasespam.espam.edu.ec/Universidad públicahttp://www.espam.edu.ec/http://revistasespam.espam.edu.ec/oai.Ecuador.1390-81031390-597Xopendoar:02025-02-25T14:38:20Revistas Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabífalse |
| spellingShingle | Microbial diversity present in wastewater from the tuna industry Pincay Cantos, María Fernanda ambiente β-galactosidasa caracterización contaminantes environment β-galactosidase characterization contaminants |
| status_str | publishedVersion |
| title | Microbial diversity present in wastewater from the tuna industry |
| title_full | Microbial diversity present in wastewater from the tuna industry |
| title_fullStr | Microbial diversity present in wastewater from the tuna industry |
| title_full_unstemmed | Microbial diversity present in wastewater from the tuna industry |
| title_short | Microbial diversity present in wastewater from the tuna industry |
| title_sort | Microbial diversity present in wastewater from the tuna industry |
| topic | ambiente β-galactosidasa caracterización contaminantes environment β-galactosidase characterization contaminants |
| url | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/312 |