In vitro activity of Bacillus subtilis and Lactobacillus brevis to reduce colonization of Salmonella entérica
The in vitro activity of indigenous microorganisms (Lactobacillus brevis, Bacillus subtilis and the mixture of both) to reduce the colonization of Salmonella enterica in vitro was determined. A DCA with factorial arrangement (3x3) was used, being the factors the microorganisms and diffusion methods...
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| Үндсэн зохиолч: | |
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| Бусад зохиолчид: | , , , |
| Формат: | article |
| Хэл сонгох: | spa |
| Хэвлэсэн: |
2022
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| Онлайн хандалт: | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/309 |
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Шошго нэмэх
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Шошго нэмэх | _version_ | 1828620682425008128 |
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| author | Fuentes Osorio, Lilian |
| author2 | Arteaga Chávez, Fátima Hurtado, Ernesto Antonio Rondón Castillo, Ana Julia Rodríguez Oliva, Marlen |
| author2_role | author author author author |
| author_facet | Fuentes Osorio, Lilian Arteaga Chávez, Fátima Hurtado, Ernesto Antonio Rondón Castillo, Ana Julia Rodríguez Oliva, Marlen |
| author_role | author |
| collection | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| dc.creator.none.fl_str_mv | Fuentes Osorio, Lilian Arteaga Chávez, Fátima Hurtado, Ernesto Antonio Rondón Castillo, Ana Julia Rodríguez Oliva, Marlen |
| dc.date.none.fl_str_mv | 2022-06-30 |
| dc.format.none.fl_str_mv | application/pdf text/html application/zip |
| dc.identifier.none.fl_str_mv | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/309 10.51260/revista_espamciencia.v13i1.309 |
| dc.language.none.fl_str_mv | spa |
| dc.publisher.none.fl_str_mv | Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López |
| dc.relation.none.fl_str_mv | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/309/306 https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/309/390 https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/309/545 |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | Revista ESPAMCIENCIA; Vol. 13 Núm. 1 (2022): REVISTA ESPAMCIENCIA 2022; 26-31 1390-8103 reponame:Revistas Escuela Superior Politécnica Agropecuaria de Manabí instname:Escuela Superior Politécnica Agropecuaria de Manabí instacron:ESPAM |
| dc.title.none.fl_str_mv | In vitro activity of Bacillus subtilis and Lactobacillus brevis to reduce colonization of Salmonella entérica Actividad in vitro de Bacillus subtilis y Lactobacillus brevis para reducir la colonización de Salmonella entérica |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo revisado por pares |
| description | The in vitro activity of indigenous microorganisms (Lactobacillus brevis, Bacillus subtilis and the mixture of both) to reduce the colonization of Salmonella enterica in vitro was determined. A DCA with factorial arrangement (3x3) was used, being the factors the microorganisms and diffusion methods (direct, filtered and diluted) in the inhibition of the confrontation of microorganisms, with the use of the Kirby-Bauer technique. Survival in bile salts performed by the CFU count, under a design that had four treatments. The microorganisms with the application of three methods against Salmonella enterica had a favorable growth at 24 hours, obtaining a greater development with Lactobacillus brevis + Bacillus subtilis by filtration method. Survival to bile salts, no variability of results was found. However, L. brevis without bile salts stood out from minute 30 to minute 120. It is concluded that both microorganisms meet physical and biological characteristics, being suitable alternatives to be used as probiotics in animal feed for animal production purposes. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | RevESPAM_ce6c8bd2f2d4e57587ae0d1c5b89a49f |
| identifier_str_mv | 10.51260/revista_espamciencia.v13i1.309 |
| instacron_str | ESPAM |
| institution | ESPAM |
| instname_str | Escuela Superior Politécnica Agropecuaria de Manabí |
| language | spa |
| network_acronym_str | RevESPAM |
| network_name_str | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| oai_identifier_str | oai:espamciencia.espam.edu.ec:article/309 |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López |
| reponame_str | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Revistas Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabí |
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| rights_invalid_str_mv | http://creativecommons.org/licenses/by-nc-sa/4.0 |
| spelling | In vitro activity of Bacillus subtilis and Lactobacillus brevis to reduce colonization of Salmonella entéricaActividad in vitro de Bacillus subtilis y Lactobacillus brevis para reducir la colonización de Salmonella entéricaFuentes Osorio, LilianArteaga Chávez, FátimaHurtado, Ernesto AntonioRondón Castillo, Ana JuliaRodríguez Oliva, MarlenThe in vitro activity of indigenous microorganisms (Lactobacillus brevis, Bacillus subtilis and the mixture of both) to reduce the colonization of Salmonella enterica in vitro was determined. A DCA with factorial arrangement (3x3) was used, being the factors the microorganisms and diffusion methods (direct, filtered and diluted) in the inhibition of the confrontation of microorganisms, with the use of the Kirby-Bauer technique. Survival in bile salts performed by the CFU count, under a design that had four treatments. The microorganisms with the application of three methods against Salmonella enterica had a favorable growth at 24 hours, obtaining a greater development with Lactobacillus brevis + Bacillus subtilis by filtration method. Survival to bile salts, no variability of results was found. However, L. brevis without bile salts stood out from minute 30 to minute 120. It is concluded that both microorganisms meet physical and biological characteristics, being suitable alternatives to be used as probiotics in animal feed for animal production purposes.Se determinó la actividad in vitro de microorganismos autóctonos (Lactobacillus brevis, Bacillus subtilis y la mezcla de ambos) para reducir la colonización de Salmonella entérica in vitro. Se utilizó un DCA con arreglo factorial (3x3) siendo los factores los microorganismos y métodos de difusión (directo, filtrado y diluido) en la inhibición del enfrentamiento de microorganismos, con el uso de la técnica Kirby-Bauer. La supervivencia en sales biliares realizada por el conteo de UFC, bajo un diseño que contó con cuatro tratamientos. Los microorganismos con aplicación de tres métodos frente a Salmonella entérica tuvieron un crecimiento favorable a las 24 horas, obteniendo un mayor desarrollo con Lactobacillus brevis + Bacillus subtilis por método de filtración. La supervivencia a sales biliares, no se encontró variabilidad de resultados. Sin embargo, el L. brevis sin sales biliares se destacó desde el minuto 30 hasta el minuto 120. Se concluye que ambos microorganismos cumplen con características físicas y biológicas, siendo alternativas idóneas para ser utilizados como probióticos en alimentación animal con fines en la producción animal.Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López2022-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtículo revisado por paresapplication/pdftext/htmlapplication/ziphttps://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/30910.51260/revista_espamciencia.v13i1.309Revista ESPAMCIENCIA; Vol. 13 Núm. 1 (2022): REVISTA ESPAMCIENCIA 2022; 26-311390-8103reponame:Revistas Escuela Superior Politécnica Agropecuaria de Manabíinstname:Escuela Superior Politécnica Agropecuaria de Manabíinstacron:ESPAMspahttps://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/309/306https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/309/390https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/309/545http://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccess2025-02-25T14:38:20Zoai:espamciencia.espam.edu.ec:article/309Institucionalhttp://revistasespam.espam.edu.ec/Universidad públicahttp://www.espam.edu.ec/http://revistasespam.espam.edu.ec/oai.Ecuador.1390-81031390-597Xopendoar:02025-02-25T14:38:20Revistas Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabífalse |
| spellingShingle | In vitro activity of Bacillus subtilis and Lactobacillus brevis to reduce colonization of Salmonella entérica Fuentes Osorio, Lilian |
| status_str | publishedVersion |
| title | In vitro activity of Bacillus subtilis and Lactobacillus brevis to reduce colonization of Salmonella entérica |
| title_full | In vitro activity of Bacillus subtilis and Lactobacillus brevis to reduce colonization of Salmonella entérica |
| title_fullStr | In vitro activity of Bacillus subtilis and Lactobacillus brevis to reduce colonization of Salmonella entérica |
| title_full_unstemmed | In vitro activity of Bacillus subtilis and Lactobacillus brevis to reduce colonization of Salmonella entérica |
| title_short | In vitro activity of Bacillus subtilis and Lactobacillus brevis to reduce colonization of Salmonella entérica |
| title_sort | In vitro activity of Bacillus subtilis and Lactobacillus brevis to reduce colonization of Salmonella entérica |
| url | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/309 |