Antibacterial activity of the oregano extract Origanum vulgare emulsioned on bovine mastitis
Aqueous extract of oregano leaves Origanum vulgare was obtained to determine its antibacterial activity in cows in production with bovine mastitis distilled by steam entrainment, with 12 and 14% concentration in phenolic compounds and it was emulsified (O/W) using two methods: ultrasound and magneti...
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2019
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| 在线阅读: | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/197 |
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| author | Bravo Loor, Johnny |
| author2 | Suárez Porto, Carlos Espinoza Vélez, Albert Muñoz Cedeño, Jesús |
| author2_role | author author author |
| author_facet | Bravo Loor, Johnny Suárez Porto, Carlos Espinoza Vélez, Albert Muñoz Cedeño, Jesús |
| author_role | author |
| collection | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| dc.creator.none.fl_str_mv | Bravo Loor, Johnny Suárez Porto, Carlos Espinoza Vélez, Albert Muñoz Cedeño, Jesús |
| dc.date.none.fl_str_mv | 2019-12-27 |
| dc.format.none.fl_str_mv | application/pdf text/html application/zip |
| dc.identifier.none.fl_str_mv | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/197 |
| dc.language.none.fl_str_mv | spa |
| dc.publisher.none.fl_str_mv | Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López |
| dc.relation.none.fl_str_mv | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/197/207 https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/197/599 https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/197/600 |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | Revista ESPAMCIENCIA; Vol. 10 Núm. 2 (2019): REVISTA ESPAMCIENCIA 2019; 71-77 1390-8103 reponame:Revistas Escuela Superior Politécnica Agropecuaria de Manabí instname:Escuela Superior Politécnica Agropecuaria de Manabí instacron:ESPAM |
| dc.title.none.fl_str_mv | Antibacterial activity of the oregano extract Origanum vulgare emulsioned on bovine mastitis Actividad antibacteriana del extracto de orégano Origanum vulgare emulsionado sobre mastitis bovina |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo revisado por pares |
| description | Aqueous extract of oregano leaves Origanum vulgare was obtained to determine its antibacterial activity in cows in production with bovine mastitis distilled by steam entrainment, with 12 and 14% concentration in phenolic compounds and it was emulsified (O/W) using two methods: ultrasound and magnetic stirring. For the diagnosis, the California test (CMT) was used and confirmed by somatic cell count (SCC) by fixed cytometry and colony- forming units (CFU) / mL in Mannitol and MacConkey agar crops for Staphyloccocus aureus and Escherichia coli respectively. The application of 5 mL of the emulsified extract via intramammary after milking and breastfeeding for 3 days with stainless steel cannulas. The trial was conducted in a completely randomized design and analysis of variance with bifactorial arrangement; The results indicate non-significant statistical differences for the CCS and CFU / mL between the concentrations (12 and 14%) of phenolic compounds. However, there actually were significant differences p <0.05 for the method of ultrasound processing in the CFU / mL for colonies of S. aureus and the CCS, so it is concluded that the product has an inhibitory effect on this bacterium. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | RevESPAM_e29361f97a3a89edc0c3e8a8d38c91dd |
| instacron_str | ESPAM |
| institution | ESPAM |
| instname_str | Escuela Superior Politécnica Agropecuaria de Manabí |
| language | spa |
| network_acronym_str | RevESPAM |
| network_name_str | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| oai_identifier_str | oai:espamciencia.espam.edu.ec:article/197 |
| publishDate | 2019 |
| publisher.none.fl_str_mv | Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López |
| reponame_str | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Revistas Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabí |
| repository_id_str | 0 |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by-nc/4.0 |
| spelling | Antibacterial activity of the oregano extract Origanum vulgare emulsioned on bovine mastitisActividad antibacteriana del extracto de orégano Origanum vulgare emulsionado sobre mastitis bovinaBravo Loor, JohnnySuárez Porto, CarlosEspinoza Vélez, AlbertMuñoz Cedeño, Jesús Aqueous extract of oregano leaves Origanum vulgare was obtained to determine its antibacterial activity in cows in production with bovine mastitis distilled by steam entrainment, with 12 and 14% concentration in phenolic compounds and it was emulsified (O/W) using two methods: ultrasound and magnetic stirring. For the diagnosis, the California test (CMT) was used and confirmed by somatic cell count (SCC) by fixed cytometry and colony- forming units (CFU) / mL in Mannitol and MacConkey agar crops for Staphyloccocus aureus and Escherichia coli respectively. The application of 5 mL of the emulsified extract via intramammary after milking and breastfeeding for 3 days with stainless steel cannulas. The trial was conducted in a completely randomized design and analysis of variance with bifactorial arrangement; The results indicate non-significant statistical differences for the CCS and CFU / mL between the concentrations (12 and 14%) of phenolic compounds. However, there actually were significant differences p <0.05 for the method of ultrasound processing in the CFU / mL for colonies of S. aureus and the CCS, so it is concluded that the product has an inhibitory effect on this bacterium. Se obtuvo extracto acuoso de hojas de orégano Origanum vulgare para determinar su actividad antibacteriana en vacas en producción con mastitis bovina destilándolo por arrastre de vapor, con 12 y 14% de concentración en compuestos fenólicos y se emulsionó (O/W) usando dos métodos: ultrasonido y agitación magnética. Para el diagnóstico se usó el test de California (CMT) y se confirmó mediante conteo de células somáticas (CCS) por citometría fija y unidades formadoras de colonias (UFC)/mL en cultivos de agares Manitol y MacConkey para Staphyloccocus aureus y Escherichia coli respectivamente. La aplicación de 5 mL del extracto emulsionado vía intramamaria posterior al ordeño y amamantamiento de la cría durante 3 días con cánulas de acero inoxidable. El ensayo se condujo en un diseño completamente al azar y análisis de varianza con arreglo bifactorial; los resultados indican diferencias estadísticas no significativas para el CCS y UFC/mL entre las concentraciones (12 y 14%) de compuestos fenólicos, no obstante, sí hubo diferencias significativas p < 0,05 para el método de elaboración ultrasonido en las UFC/mL para colonias de S. aureus y el CCS, por lo que se concluye que el producto tiene efecto inhibidor sobre esta bacteria. Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López2019-12-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtículo revisado por paresapplication/pdftext/htmlapplication/ziphttps://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/197Revista ESPAMCIENCIA; Vol. 10 Núm. 2 (2019): REVISTA ESPAMCIENCIA 2019; 71-771390-8103reponame:Revistas Escuela Superior Politécnica Agropecuaria de Manabíinstname:Escuela Superior Politécnica Agropecuaria de Manabíinstacron:ESPAMspahttps://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/197/207https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/197/599https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/197/600http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccess2025-02-25T14:36:04Zoai:espamciencia.espam.edu.ec:article/197Institucionalhttp://revistasespam.espam.edu.ec/Universidad públicahttp://www.espam.edu.ec/http://revistasespam.espam.edu.ec/oai.Ecuador.1390-81031390-597Xopendoar:02025-02-25T14:36:04Revistas Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabífalse |
| spellingShingle | Antibacterial activity of the oregano extract Origanum vulgare emulsioned on bovine mastitis Bravo Loor, Johnny |
| status_str | publishedVersion |
| title | Antibacterial activity of the oregano extract Origanum vulgare emulsioned on bovine mastitis |
| title_full | Antibacterial activity of the oregano extract Origanum vulgare emulsioned on bovine mastitis |
| title_fullStr | Antibacterial activity of the oregano extract Origanum vulgare emulsioned on bovine mastitis |
| title_full_unstemmed | Antibacterial activity of the oregano extract Origanum vulgare emulsioned on bovine mastitis |
| title_short | Antibacterial activity of the oregano extract Origanum vulgare emulsioned on bovine mastitis |
| title_sort | Antibacterial activity of the oregano extract Origanum vulgare emulsioned on bovine mastitis |
| url | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/197 |