Selección de cepas bacterianas acumuladoras de boro en suelos cultivados con brócoli en la provincia de Cotopaxi-Ecuador
The rapid population growth has spurred the increased agricultural activities with consequences involved such as the indiscriminate use of pesticides and chemical fertilizers; raising negative impacts on ecosystems. Boron is a micronutrient needed in commercial fertilizers for optimal crop developme...
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| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | http://dspace.udla.edu.ec/handle/33000/6764 |
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Adicionar Tag | Resumo: | The rapid population growth has spurred the increased agricultural activities with consequences involved such as the indiscriminate use of pesticides and chemical fertilizers; raising negative impacts on ecosystems. Boron is a micronutrient needed in commercial fertilizers for optimal crop development. When the boron concentration exceeds the maximum on the soil (1 to 9 ppm), depending on the crop, it becomes toxic causing environmental and agricultural problems. The impact of excessive concentrations of this metalloid is reflected in reducing soil quality, functionality and performance. In this study, in order to identify a method to reduce high boron concentrations in agricultural soils, bacterial strains that accumulate this element were isolated and identified from broccoli crops soil samples in Poaló town, Cotopaxi – Ecuador. Forty bacterial were initially chosen and exposed to boron concentrations between 4 and 750 ppm in Luria Bertani (LB) agar, and finally only five strains were selected to test bioaccumulation and biochemical enzymatic identification. BorA16, BorB27, BorB29, BorC310 and BorD46 that tolerate boron concentration levels of at least 750 ppm, and bioaccumulated 55%, 57.5%, 62.5%, 37.5% and 55% ppm respectively, of the concentration at which were exposed. Results of this analysis show that selected bacteria could belong to the genera: Bacillus spp, Brevibacterium spp. and Pseudomonas spp., and that they could be considered suitable for bioremediation of agricultural soils affected by excessive boron concentration levels. |
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