Estandarización de un protocolo de micropropagación de papaya (carica papaya l.) en un sistema de inmersión temporal de vasos gemelos (bit)

Papaya production is affected due to sexual variability in the seed. The methods of conventional propagation involve the maintenance of large land plantations to supply the consumers demand. An alternative is the micropropagation in the bioreactor of temporary immersion of twin vessels (BIT). The purpose of this work is the establishment of a disinfection methodology for the introduction of axillary buds and the evaluation of immersion time and inoculum density in the BIT to obtain a higher multiplication rate. Disinfection of the plant material was carried out with 70 percent ethanol for 1 minute, 2 percent sodium hypochlorite for 5, 6 and 8 min, and washed with sterile distilled water. For the introduction, Murashige and Skoog medium was used with 1 mg.L less 1 of gibberellic acid and 2 mg.L less 1 of kinetin; in the same way for multiplication supplemented with 0.5 mg.L less 1 of benzyl adenine, 0.5 mgL less 1 of indoleacetic acid and 0.3 mgL less 1 of gibberellic acid; for 21 days the multiplication rate, number of leaves, height and diameter of the stem was evaluated. The plant material was transferred to the BIT to evaluate the immersion time (1 and 2 min) and density of the inoculum (4 and 8) in 200 ml of liquid multiplication medium, with an immersion frequency of 6 h, photoperiod of 12 h of lighting per day and 28 degrees Celsius. In the disinfection with 2 percent sodium hypochlorite for 6 and 8 min, 100 percent survival without oxidation was achieved. In the introduction stage, 96.4 percent survival was obtained; similarly in the stage of multiplication in semi-solid medium (83.33 percent), with a number of leaves 1.88 more less 0.02, height 1.12 more less 0.04 cm, diameter of 0.43 more less 0.01 cm and a multiplication rate of 1.20 more less 0.02. In the BIT, in a time of 2 min of immersion every 6 h with an inoculum density of 8, a number of leaves was obtained 6.03 more less 0.04, height 1.65 more less 0.10 cm, diameter 0.73 more less 0.02 and a multiplication rate of 5.05 more less 0.06. This work in addition to generating an academic contribution, could contribute to the strengthening of the productive sector, offering them seedlings with the suitable sex (hermaphrodite), with high genetic and phytosanitary quality.

• Автор: Gómez Carrera, Ruth Elena (author)
• Формат: bachelorThesis
• Мова: spa
• Опубліковано: 2018
• Предмети: BIORREACTORES; MICROPROPAGACIÓN; PAPAYA
• Онлайн доступ: http://dspace.udla.edu.ec/handle/33000/9079