Evaluación de la calidad del agua embotellada en la provincia bolívar a través de análisis biomoleculares (PCR) y cultivos microbiológicos

The present research project entitled "Evaluation of Bottled Water Quality in the Bolivar Province through Biomolecular Analysis (PCR) and Microbiological Cultures, aims to evaluate the microbiological quality of bottled water from different companies in the Bolivar Province through biomolecular analysis (PCR) and microbiological cultures, having as main objectives to determine the quality of water through the use of specific culture media for the development of microorganisms. To characterize the presence of Listeria monocytogenes, Salmonella spp. and Escherichia coli by means of molecular biology techniques (PCR) Polymerase Chain Reaction in final time. A completely randomized design (CRD) with factorial arrangement A x B (5 x 2) with three replicates was used. The molecular technique was the result after purification of microorganisms obtained from the samples by means of selective and differential culture media, all of them in CFU/mL units. With the use of these two methodologies, a total of 99 samples of bottled water were analyzed equally from each of the water bottling companies in the different cantons of the Bolivar province. Specific primers of the Invitrogen brand were used to detect the serotype of both Listeria monocytogenes, Escherichia coli with its serotype O157:H7 and Salmonella spp using positive targets of the three microorganisms that were acquired by the research department for a better result. Listeria monocytogenes was detected in 91,6% (12/11), for Escherichia coli 75% (9/99) and for Salmonella spp. 50% (6/11) for each of the samples, which were interpreted by electrophoresis. The polymerase chain reaction (PCR) was more sensitive for Escherichia coli, O157:H7 and Listeria monocytogenes giving accurate results in conjunction with the positive blanks. In conclusion, the presence of these bacteria in bottled water can be considered a health and welfare risk factor for the consumer.

• Автор: Llanos García, Francisco Andrés (author)
• Формат: bachelorThesis
• Мова: spa
• Опубліковано: 2024
• Предмети: AGUA EMBOTELLADA; BIOMOLECULARES; MICROBIOLÓGICOS; CALIDAD
• Онлайн доступ: https://dspace.ueb.edu.ec/handle/123456789/6735