Determinación de la actividad anticrobiana del hongo shiitake (Lentinula edodes), cultivado en residuos lignocelulósicos

The objective of the present investigation was to determine the antimicrobial activity of the shiitake fungus (Lentinula edodes), cultivated in lignocellulosic residues, establishing as factors of study: the parts of the mushroom of the Lentinula edodes (pileus and stipe), and types of solvent (chloroform, ethanol, acetate style, water + 50% ethanol). Mushrooms grown on lignocellulosic residues, were harvested, dehydrated at room temperature and divided into pileus and stipe, these were analyzed separately, they showed variations among themselves in the moisture content which varied very little, the ash content for the Stipe was much larger (7.9 g / 100g). The protein content was higher for the pileus (24.2 g / 100g), while the fiber was higher for the stipe (22.8 g / 100g). The fat content in the fungus Lentinula edodes was very low (<3%). From the stipe and pileus of the pulverized fungus extracts were obtained using four different solvents, the extract that had the highest yield was T1 (67 mL) corresponding to the stipe + chloroform, however the extraction with ethanol in the stipe of the fungus was the most efficient process because the Ethanoic extracts of the stipe obtained the highest inhibition zones against the three pathogenic bacteria. Ethanol better retained the bioactive agents of the fungus spindle Lentinula edodes. The antimicrobial efficiency of the extracts of the fungus was tested on pathogenic bacteria, obtaining that the T2 treatment (stipe + ethanol) presented better results in the plate-plate antibiogram technique inhibiting the growth of the bacteria: Listeria spp (Gram positive) with a inhibition halo of 12.7 mm, Salmonella spp (Gram negative) with a halo of inhibition of 9.3 mm and Escherichia coli (Gram negative) with a halo of inhibition of 8.0 mm.

• Hovedforfatter: Sanabria Hinojoza, Jordani Eduardo (author)
• Format: bachelorThesis
• Sprog: spa
• Udgivet: 2018
• Fag: ANTIMICROBIANA; PILEO; ESTIPE; DISOLVENTE
• Online adgang: http://dspace.ueb.edu.ec/handle/123456789/2231