Obtención y caracterización de principios bioactivos a partir de las hojas y tallo de matico (Salvia corrugata) mediante el método de fluidos super críticos

The main purpose of the research was to obtain bioactive principles from matico leaves and stems using the supercritical fluid extraction method. Among the specific objectives was to characterize the raw material of matico leaves and stems, finding that 76.89% corresponded to the leaves and 83.49% to the stems, indicating that both parts of the matico plant had a high-water content and that there was a noticeable difference between them. To determine the best pressures for the extraction of bioactive principles using supercritical fluids, statistical tests such as ANOVA were performed, and it was found that the type of sample (factor A) significantly influenced the yield with a p-value of 0.0070, confirming with 95% confidence that there is a statistically significant difference in the amount of extract between the leaves and stems. on the contrary, factor B (pressure) had a p-value of 0.7733, indicating that the different pressures (200, 300, 400 Bar) do not generate significant changes in yield; the bioactive principles obtained from the leaves and stem of matico were characterized and quantified by GC-MS, obtaining that for the SFE extract 200, 300, 400 Bar in matico leaves, compounds such as 2,4-Heptadiene, 2,6-dimethyl- with 57.7%, Drimenol with 45.14%, and 2,4-Heptadiene, 2,6dimethyl- with 54.06% were the major compounds in matico leaves, indicating a high content of terpenoids and volatile compounds that are part of essential oils; and in the stem of SFE extract 200, 300, 400 Bar, 2,4-Heptadiene, 2,6-dimethyl- with 57.7%, Drimenol with 45.14% and 2,4-Heptadiene, 2,6 -dimethyl- with 54.06% are volatile compounds that, due to their high concentration, influence the aromatic, sensory, and technical properties of the extract. Finally, the leaf extracts, such as matico stem (Salvia corrugata), were able to inhibit the growth of Escherichia coli bacteria, although the effectiveness varies slightly depending on the type of sample, the method, and the amount applied.

• Autore principale: Angamarca Sosa, María del Carmen (author)
• Altri autori: Flores Suárez, Dayanara de los Ángeles (author)
• Natura: bachelorThesis
• Pubblicazione: 2025
• Soggetti: MATICO (SALVIA CORRUGATA); BIOACTIVOS; TALLO; HOJA; EXTRACTO; COMPUESTOS VOLÁTILES; ESCHERICHIA COLI.
• Accesso online: https://dspace.ueb.edu.ec/handle/123456789/8818