Evaluación de la digestibilidad gastrointestinal in vitro y la actividad antioxidante de concentrados e hidrolizados proteicos de matrices vegetales
The main objective of this research work was the evaluation of gastrointestinal digestibility and antioxidant activity in vitro in protein concentrates and hydrolyzates of two different plant matrices: (yellow pitahaya (Hylocereus megalanthus) and purple corn (Zea mays L.)), for which, the protein w...
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| Format: | bachelorThesis |
| Language: | spa |
| Published: |
2020
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| Online Access: | http://dspace.ueb.edu.ec/handle/123456789/3618 |
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Add Tag | Summary: | The main objective of this research work was the evaluation of gastrointestinal digestibility and antioxidant activity in vitro in protein concentrates and hydrolyzates of two different plant matrices: (yellow pitahaya (Hylocereus megalanthus) and purple corn (Zea mays L.)), for which, the protein was concentrated by means of isoelectric precipitation at pH values of solubility of 8,0 and 4,0 of precipitation for the seeds of yellow pitahaya; and 10,0 solubility and 4,0 precipitation for purple corn. The protein content of the flour of yellow pitahaya seeds was 26,65%; and 8,25% of purple corn flour, as well as 81,31% and 38,48% in concentrates, respectively. The content of polyphenols in the flours, concentrates and protein hydrolyzates of the two matrices studied was determined, where values of 626,63 mg GAE/g of sample were found in the flour of yellow pitahaya seeds and 2390,11 mg GAE/g of sample in the purple corn concentrate, being these the highest among the other analyzed forms. The antioxidant activity of proteins, in flours, concentrates and protein hydrolyzates using the FRAP method, where the highest values were 51,50 µM ET/g of sample in yellow pitahaya seed meal, as well as 54, 44 µM ET/g of sample for the case of purple corn concentrate, keeping a directly proportional relationship with the content of polyphenols found. The in vitro digestibility of the proteins was evaluated using the SDS-PAGE electrophoresis technique. Proteins were partially digested in both gastric and duodenal digestion. Likewise, by means of electrophoresis the protein profile of the two matrices was identified, finding bands with molecular weights of between 11,04 to 105,56 kDa in the protein concentrate of the yellow pitahaya seeds and from 8,54 to 64,94 a kDa in the protein concentrate of purple corn |
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