Determinación in vitro de la Digestibilidad Gástrica y Duodenal en concentrados proteicos de moringa oleifera
The attribution of medicinal and industrial properties of Moringa oleifera has increased its consumption in recent years. In Ecuador it is cultivated in subtropical and tropical zones presenting high levels of adaptation due to the similarity of climatic conditions with respect to its place of origi...
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| 格式: | bachelorThesis |
| 语言: | spa |
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2019
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| 在线阅读: | http://dspace.ueb.edu.ec/handle/123456789/2987 |
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添加标签 | 总结: | The attribution of medicinal and industrial properties of Moringa oleifera has increased its consumption in recent years. In Ecuador it is cultivated in subtropical and tropical zones presenting high levels of adaptation due to the similarity of climatic conditions with respect to its place of origin attributed to the North East and South of India. In the province of Bolivar, dried and powdered leaves are cultivated and sold as a nutritional supplement rich in proteins, but the quantity (percentage) and quality (characterization) of them are completely unknown. Contraindications to health have been reported worldwide on the consumption of this plant being one of the most relevant gastric problems. In this study it was possible to quantify the percentage of protein through an elemental analyzer, obtaining a 25.77% in the processed flour. On the other hand, the protein percentage of the concentrates obtained by the modified isoelectric point method determined that pH 5.0 is the most recommended for the extraction of proteins, obtaining 40.66%. As a result of the SDS-PAGE electrophoresis characterization, 4 proteins of 12, 46, 53 and 218 kDa were identified. For the simulation of the gastrointestinal process in vitro, pepsin, pancreatin and bile salts were used, performing pH variations to simulate an adult with gastric problems (pH 1.2), an adult healthy (pH 2.0) and a non-lactating child (pH3.2). ) Once the gastrointestinal simulation process was completed, the proteins were again characterized, finding that in non-lactating children, 3 of the 4 proteins whose molecular weights are 12, 45 and 52 kDa respectively; they have not been completely digested, this suggests that they could have undesirable consequences for their health, being able to manifest from a simple diarrheic process to an allergic reaction process, however more studies and in vivo tests would be needed to corroborate this information. Key words: Moringa oleifera, isoelectric point, gastrointestinal digestibility, protein electrophoresis, in vitro. |
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