Determinación de la efectividad antimicrobiana del aceite esencial del Orégano (Origanum vulgare) frente a cepas de escherichia coli aislada de mastitis bovina
The present investigation has as objective; To determine the antimicrobial effectiveness of the essential oil of oregano against strains of Escherichia coli isolated from bovine mastitis, in the general laboratory of the Faculty of Agricultural Sciences of the State University of Bolivar, through th...
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| Natura: | bachelorThesis |
| Lingua: | spa |
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2023
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| Accesso online: | https://dspace.ueb.edu.ec/handle/123456789/4910 |
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| author | Hernández Ramírez, Angel Jairo |
| author_facet | Hernández Ramírez, Angel Jairo |
| author_role | author |
| collection | Repositorio Universidad Estatal de Bolivar |
| dc.contributor.none.fl_str_mv | Ramón Curay, Edison Riveliño |
| dc.creator.none.fl_str_mv | Hernández Ramírez, Angel Jairo |
| dc.date.none.fl_str_mv | 2023-02-24T16:31:07Z 2023-02-24T16:31:07Z 2023 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://dspace.ueb.edu.ec/handle/123456789/4910 |
| dc.language.none.fl_str_mv | spa |
| dc.publisher.none.fl_str_mv | Guaranda. Universidad Estatal de Bolívar. Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente. Carrera de Medicina Veterinaria y Zootecnia |
| dc.relation.none.fl_str_mv | FCCAG.MVZ;234 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Universidad Estatal de Bolivar instname:Universidad Estatal de Bolivar instacron:UEB |
| dc.subject.none.fl_str_mv | EFECTIVIDAD ANTIMICROBIANA ACEITE ESENCIAL ESCHERICHIA COLI |
| dc.title.none.fl_str_mv | Determinación de la efectividad antimicrobiana del aceite esencial del Orégano (Origanum vulgare) frente a cepas de escherichia coli aislada de mastitis bovina |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
| description | The present investigation has as objective; To determine the antimicrobial effectiveness of the essential oil of oregano against strains of Escherichia coli isolated from bovine mastitis, in the general laboratory of the Faculty of Agricultural Sciences of the State University of Bolivar, through the analysis of samples from the farm “La Felicidad” located in the Upan parish, belonging to the province of Chimborazo, methodologically for the development of this research began with the extraction of the essential oil of oregano by steam distillation, The yield and chemical characteristics were evaluated through the quantification of volatile compounds by gas chromatography with mass spectrometry (GC-MS), in addition, the determination of its antimicrobial activity at concentrations of 20%, 40%, 60% and Enrofloxacin 5μg as a positive control against 20 strains of Escherichia coli, identifying the minimum concentration required to inhibit their growth with findings of inhibition halos with averages greater than 8 mm. As part of the results found, it was observed that the oil yield was 0.84%, highlighting that within the composition formic acid and p-Cymene were identified in 66.63% and 11.54% respectively, observing that the treatments expressed a highly significant effect p<0.05, where T3 (60%) expressed the highest average in the diameter of the inhibition halo with 62. 40 mm, establishing that the minimum inhibitory concentration was 3% since it showed an average of 9 mm of inhibition halo, concluding based on this background that oregano essential oil has antimicrobial effectiveness against strains of Escherichia coli causing bovine mastitis in in-vitro assays using the Kirby-Bauer technique. |
| eu_rights_str_mv | openAccess |
| format | bachelorThesis |
| id | UEB_bd410ea53ea8aa2ebd0fa83c31dbc03b |
| instacron_str | UEB |
| institution | UEB |
| instname_str | Universidad Estatal de Bolivar |
| language | spa |
| network_acronym_str | UEB |
| network_name_str | Repositorio Universidad Estatal de Bolivar |
| oai_identifier_str | oai:dspace.ueb.edu.ec:123456789/4910 |
| publishDate | 2023 |
| publisher.none.fl_str_mv | Guaranda. Universidad Estatal de Bolívar. Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente. Carrera de Medicina Veterinaria y Zootecnia |
| reponame_str | Repositorio Universidad Estatal de Bolivar |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Repositorio Universidad Estatal de Bolivar - Universidad Estatal de Bolivar |
| repository_id_str | 1735 |
| spelling | Determinación de la efectividad antimicrobiana del aceite esencial del Orégano (Origanum vulgare) frente a cepas de escherichia coli aislada de mastitis bovinaHernández Ramírez, Angel JairoEFECTIVIDADANTIMICROBIANAACEITE ESENCIALESCHERICHIA COLIThe present investigation has as objective; To determine the antimicrobial effectiveness of the essential oil of oregano against strains of Escherichia coli isolated from bovine mastitis, in the general laboratory of the Faculty of Agricultural Sciences of the State University of Bolivar, through the analysis of samples from the farm “La Felicidad” located in the Upan parish, belonging to the province of Chimborazo, methodologically for the development of this research began with the extraction of the essential oil of oregano by steam distillation, The yield and chemical characteristics were evaluated through the quantification of volatile compounds by gas chromatography with mass spectrometry (GC-MS), in addition, the determination of its antimicrobial activity at concentrations of 20%, 40%, 60% and Enrofloxacin 5μg as a positive control against 20 strains of Escherichia coli, identifying the minimum concentration required to inhibit their growth with findings of inhibition halos with averages greater than 8 mm. As part of the results found, it was observed that the oil yield was 0.84%, highlighting that within the composition formic acid and p-Cymene were identified in 66.63% and 11.54% respectively, observing that the treatments expressed a highly significant effect p<0.05, where T3 (60%) expressed the highest average in the diameter of the inhibition halo with 62. 40 mm, establishing that the minimum inhibitory concentration was 3% since it showed an average of 9 mm of inhibition halo, concluding based on this background that oregano essential oil has antimicrobial effectiveness against strains of Escherichia coli causing bovine mastitis in in-vitro assays using the Kirby-Bauer technique.La presente investigación tiene como objetivo; determinar la efectividad antimicrobiana del aceite esencial del Orégano frente a cepas de Escherichia coli aislada de mastitis bovina, en el laboratorios general de la facultad de Ciencias Agropecuarias de la Universidad Estatal de Bolívar, mediante el análisis de muestras procedentes de la finca la Felicidad ubicada en la parroquia Ulpan, perteneciente a la provincia de Chimborazo, metodológicamente para el desarrollo de la presente investigación se inicio con la extracción del aceite esencial de orégano mediante destilación por arrastre de vapor, evaluando su rendimiento y características químicas mediante la cuantificación de los compuesto volátiles por cromatografía de gases con espectro de masa (GC-MS), además, la determinación de su actividad antimicrobiana a las concentraciones de 20%, 40%, 60% y Enrofloxacina 5μg como testigo positivo frente a 20 cepas de Escherichia coli, identificando la concentración mínima requerida para inhibición de su crecimiento con hallazgos de halos de inhibición con medias mayores a 8 mm. Como parte de los resultados encontrados se observo que el rendimiento del aceite fue de 0.84%, destacando que dentro de la composición se identificó al ácido fórmico y p-Cymene en un 66.63% y 11.54% respectivamente, observando que los tratamientos expresaron un efecto altamente significativo p<0.05, donde el T3 (60%) expresó el mayor promedio en el diámetro del halo de inhibición con 62.40 mm, estableciendo que la concentración mínima inhibitoria fue de 3% ya que esta ostentó un promedio de 9 mm de halo de inhibición, concluyendo en base a estos antecedentes que el aceite esencial de orégano posee efectividad antimicrobiana frente a cepas de Escherichia coli causante de mastitis bovina en ensayos in-vitro mediante la técnica de Kirby-Bauer.Guaranda. Universidad Estatal de Bolívar. Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente. Carrera de Medicina Veterinaria y ZootecniaRamón Curay, Edison Riveliño2023-02-24T16:31:07Z2023-02-24T16:31:07Z2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfhttps://dspace.ueb.edu.ec/handle/123456789/4910spaFCCAG.MVZ;234info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Estatal de Bolivarinstname:Universidad Estatal de Bolivarinstacron:UEB2025-11-30T17:58:06Zoai:dspace.ueb.edu.ec:123456789/4910Institucionalhttps://dspace.ueb.edu.ec/Universidad públicahttps://www.ueb.edu.ec/https://dspace.ueb.edu.ec/oai.Ecuador...opendoar:17352026-02-23T18:27:30.723774Repositorio Universidad Estatal de Bolivar - Universidad Estatal de Bolivartrue |
| spellingShingle | Determinación de la efectividad antimicrobiana del aceite esencial del Orégano (Origanum vulgare) frente a cepas de escherichia coli aislada de mastitis bovina Hernández Ramírez, Angel Jairo EFECTIVIDAD ANTIMICROBIANA ACEITE ESENCIAL ESCHERICHIA COLI |
| status_str | publishedVersion |
| title | Determinación de la efectividad antimicrobiana del aceite esencial del Orégano (Origanum vulgare) frente a cepas de escherichia coli aislada de mastitis bovina |
| title_full | Determinación de la efectividad antimicrobiana del aceite esencial del Orégano (Origanum vulgare) frente a cepas de escherichia coli aislada de mastitis bovina |
| title_fullStr | Determinación de la efectividad antimicrobiana del aceite esencial del Orégano (Origanum vulgare) frente a cepas de escherichia coli aislada de mastitis bovina |
| title_full_unstemmed | Determinación de la efectividad antimicrobiana del aceite esencial del Orégano (Origanum vulgare) frente a cepas de escherichia coli aislada de mastitis bovina |
| title_short | Determinación de la efectividad antimicrobiana del aceite esencial del Orégano (Origanum vulgare) frente a cepas de escherichia coli aislada de mastitis bovina |
| title_sort | Determinación de la efectividad antimicrobiana del aceite esencial del Orégano (Origanum vulgare) frente a cepas de escherichia coli aislada de mastitis bovina |
| topic | EFECTIVIDAD ANTIMICROBIANA ACEITE ESENCIAL ESCHERICHIA COLI |
| url | https://dspace.ueb.edu.ec/handle/123456789/4910 |