Micropropagación de explantes de dos variedades de rosas (Rosa sp.) en cultivo in vitro, utilizando tres tipos de fitorreguladores en tres dosis
Ecuador is the third largest exporter of flowers in the world after Holland and Colombia. It has a wide range of varieties of roses (Rosa sp.) which makes it accepted and successful in the international market, with almost 300 varieties with high export expectations. It should be noted that there is...
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| Формат: | bachelorThesis |
| Мова: | spa |
| Опубліковано: |
2023
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| Предмети: | |
| Онлайн доступ: | https://dspace.ueb.edu.ec/handle/123456789/5393 |
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| Резюме: | Ecuador is the third largest exporter of flowers in the world after Holland and Colombia. It has a wide range of varieties of roses (Rosa sp.) which makes it accepted and successful in the international market, with almost 300 varieties with high export expectations. It should be noted that there is a great variety of ornamental plants, of which the rose has become one of the most cultivated ornamental plants not only for decorative use, but also for food use, recreation and aesthetic satisfaction, representing one of the main products in the commercial market of floriculture. The present research was carried out in the sector of Laguacoto II, Guaranda canton, Bolivar province. The micropropagation of explants of two varieties of roses was analyzed using three types of phytoregulators at three doses. The objectives were: i) To establish an in vitro culture medium suitable for the propagation of roses from axillary buds. ii) To find the necessary concentrations of growth regulators for the in vitro multiplication of roses in the culture medium. ii) To determine the percentage of survival of explants propagated in vitro. Analysis of variance, Tukey's test at 1%, was carried out to compare the averages between treatments and factors under study (AxBxC). The main results obtained showed that the treatments with the highest number of shoots per explant were T1 Explorer + benzyl adenine + 3 mg/lt and T14 Quicksand + kinetin + 5 mg/lt with 5 shoots. In the cost-benefit ratio, the best treatment with a cost benefit is T1 Explorer + benzyl adenine + 3 mg/lt, i.e. for each unit invested there is a gain of $3.79. Followed by the treatment T14 Quicksand + kinetin + 5 mg/lt with a profit for each unit invested of $0.85. |
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