Evaluación de explantes en dos variedades de buganvilla (Bougainvillea glabra C.) mediante multiplicación in vitro, con la aplicación de tres dosis de citoquininas, Laguacoto II, provincia de Bolívar

The Bougainvillea, scientifically known as Bougainvillea glabra C., is a popular ornamental plant valued for its vibrant and striking blooms. Its ability to adapt to different climatic conditions and its use in landscaping make it an ideal subject for research in plant propagation. This study focused on evaluating the effectiveness of different types and doses of cytokinins in the in vitro multiplication of Bougainvillea. The objectives of the study were: i) To determine which variety of Bougainvillea is most effective for in vitro propagation among the two analyzed in the research. ii) To identify the most suitable cytokinin and dose for optimal explant development. iii) To establish the Benefit-Cost ratio for each treatment. The research centered on two specific types of this species (purple and white), using an experimental design that compared the effects of three different cytokinin doses (0, 3, and 5 mg/L) on explant development. The methodology implemented included a randomized block design with six distinct treatments. Each treatment was presented as the interaction between factor A (Bougainvillea types) and factor B (Cytokinin doses). The collected data indicated that one Bougainvillea type (white) showed a more favorable response in terms of the number of shoots, root length, and other growth parameters under certain cytokinin concentrations. Through this research, the cytokinin and dose (5 mg/L) that resulted in the greatest explant development were identified. In the DBL (Days to Bud Lengthening), treatments T1 and T3 showed intermediate times of 25 and 30 days, respectively. In the NHB (Number of Leaves per Shoot) variable, T3, with an average of 5 leaves per shoot, demonstrated the best performance. In the DER (Days to Root Emergence), the results showed that treatments T2 (Purple + 3 mg/L), T3 (Purple + 5 mg/L), and T6 (White + 5 mg/L) required 38, 45, and 38 days, respectively, for root emergence. When analyzing the Benefit-Cost ratio for each treatment, it was found that T3 was the most profitable, yielding a $1.96 gain for every dollar invested. This was determined by considering the total cost of the treatment and the gross income generated by the produced shoots.

• Autor principal: Quingatuña Gómez, Víctor Raúl (author)
• Format: bachelorThesis
• Publicat: 2025
• Accés en línia: https://dspace.ueb.edu.ec/handle/123456789/8121