Micro propagación in Vitro de plantas de Tomate de Árbol Silvestre (Solanum sp.) con dos tipos de fitorreguladores, aplicados en tres dosis

The cultivation of tomatoes tree is very important all over the world due to its nutritional and organoleptic importance. This fruit is one of the most significant items due to its high demand, being a catalyst of the world economy. In our country in the inter-Andean region is where this fruit is produced the most, especially in the province of Tungurahua. It is one of the crops that presents the most phytosanitary problems and it is very susceptible to the nematodes. In this sense, the wild ecotypes presenting high tolerance to root health problems, it can serve as a genetic basis for Breeding processes, generating portal grafts for the less tolerant cultivars, allowing to improve fruit quality and avoid the excessive use of pesticides. Biotechnology contributes to solve these problems with productive improvement strategies such as the use of a rustic wild ecotype of the Inter-Andean alley, tolerant to nematode attack along with the advantages of asepsis offered by in vitro propagation. The objectives of this research were: I) To establish which phytogulator would provide a greater development of the explants. II) Evaluate the dosage that allows a greater number of explants. This work was developed in the biotechnology laboratory of the UEB, Laguacoto II farm, Guaranda canton, Bolívar province. A completely randomized design (DCA) was used, with 6 treatments and 3 replicates. We performed variance analysis, test of tukey at 5%, test to compare averages, polynomial trends and correlation and simple regression analyzes. The most important results were: a higher number of shoots, number of leaves, shoot height and root volume in the treatments with Bencil adenine (A1). It was determined that the best treatment was T2 (A1B2) with an average of 3 outbreaks per explant, 4 leaves per outbreak and 3 cm in height for each outbreak. The variables that increased the development of the explants were: NBE, AB and Dose. Finally, it was possible to generate technological alternatives for the production of wild tomato plants with the idea of generating portal grafts with a high percentage of development, a higher number of high quality seedlings, and genetic rusticity providing phytosanitary tolerance.

• मुख्य लेखक: Pazmiño Gaibor, Alvaro Xavier (author)
• स्वरूप: bachelorThesis
• भाषा: spa
• प्रकाशित: 2017
• विषय: BIOTECNOLOGÍA; ECOTIPO; GENOTIPO; EXPLANTE
• ऑनलाइन पहुंच: http://dspace.ueb.edu.ec/handle/123456789/1833