Efecto de Hypocrea lixii sobre el tizón temprano del tomate Alternaria solani en condiciones de invernadero Guayas 2014

The present research work was carried out from the first week of August, 2016 until the end of January, 2017 in Daule canton, Guayas province, in the premises of the in-vitro culture laboratory AGROVITROPARIS; Which is located between the geographical coordinates 1 51 '37.77' (South Latitu...

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Hoofdauteur: Tandazo Falquez, Nelka (author)
Formaat: masterThesis
Taal:spa
Gepubliceerd in: 2015
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Online toegang:http://repositorio.ug.edu.ec/handle/redug/17756
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Samenvatting:The present research work was carried out from the first week of August, 2016 until the end of January, 2017 in Daule canton, Guayas province, in the premises of the in-vitro culture laboratory AGROVITROPARIS; Which is located between the geographical coordinates 1 51 '37.77' (South Latitude), 79º 58 '34.42 "(Western Longitude) and 15 m.s.n.m. The objective of this study was to evaluate the effect of chitosan as a growth promoter to be added to the culture medium for orchids Cattleyas spp. As starting plant material axillary and apical buds were used as explants, whose mother plants presented good agronomic characteristics; These were placed in flasks with necessary nutrients necessary for their development; and chitosan doses to be evaluated were (0mg / L, 70mg / L, 90mg / L, 150mg / L, 180mg / L, 200mg / L, 250mg / L). The fully randomized blocks design was used with four replicates and seven treatments. According to the results obtained, we conclude that: Seven treatments of 250 mg / L of chitosan added to the culture medium achieved the best results in all the variables evaluated due to the use of a higher dose of chitosan. A complete plant was obtained with a germination percentage of 93.75%, 5.25 shoots, 5.75 leaves, 3.75 roots, a leaf width of 2.20 cm and vigorous roots of 3.43 cm and less than 1.00 contamination. The application of chitosan to the culture medium is a favorable option in the in vitro propagation of the orchid Cattleya sp, significantly improving the root development in a short period of time.