Efecto antimicrobiano del alcohol al 70% como desinfectante de resinas compuestas. Universidad Nacional de Chimborazo, 2018
The objective of this investigation was to evaluate antimicrobial effect by employing alcohol 70%, as a disinfectant on composite resin syringes. In the National University of Chimborazo, 2018. This study was carried out by taking one-hundred-twenty-one resin syringe samples, before and after disinf...
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| Format: | bachelorThesis |
| Language: | spa |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://dspace.unach.edu.ec/handle/51000/5760 |
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Add Tag | Summary: | The objective of this investigation was to evaluate antimicrobial effect by employing alcohol 70%, as a disinfectant on composite resin syringes. In the National University of Chimborazo, 2018. This study was carried out by taking one-hundred-twenty-one resin syringe samples, before and after disinfection with alcohol 70%. It was used as a disinfectant solution, five samples as part of the control test. A sample was taken from a syringe from a new resin syringe exposed to the environment. It was used as a negative control. Sampling was performed randomly in three shifts of the medical clinic of the university (from 7h00 to 11h00, from 11h00 to 15h00 and from 15h00 to 19h00). The same samples were taken in duplicate before and after their disinfection. By using lab cultivation means such as blood agar, the presence of microbial load was observed. After that, Gram stain was prepared, microbial load was observed under an optical microscope; according to its morphological characteristics, these samples were reseeded in selective mean, therefore Agar, Salty Mannitol, Macconkey and Sabouraud with Chloramphenicol were employed. The obtained results determined that there is contamination in the entire sample. In addition to this, based on the statistical significance of the Pearson Chi-square test, a significance value greater than 0.05 was observed (p = 0.311). It was concluded that, alcohol, in its 70% concentration, is not an adequate disinfectant since the levels of contamination are reduced proportionally to the microbial load. Consequently, it does not achieve the complete elimination of the microorganisms |
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