Procesamiento de tejidos humanos mediante la utilización de coloraciones de rutina
ABSTRACT Human tissues are colorless and therefore there is a need to stain them to observe their morphological characteristics. The research`s work objective was to update information on processing in human tissues through the use of routine stains, for their respective diagnosis. The project has b...
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| Format: | bachelorThesis |
| Język: | spa |
| Wydane: |
2021
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| Hasła przedmiotowe: | |
| Dostęp online: | http://dspace.unach.edu.ec/handle/51000/7975 |
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Dodaj etykietę | Streszczenie: | ABSTRACT Human tissues are colorless and therefore there is a need to stain them to observe their morphological characteristics. The research`s work objective was to update information on processing in human tissues through the use of routine stains, for their respective diagnosis. The project has been carried out under a descriptive-level bibliographic documentary design, within a qualitative, non-experimental and cross-sectional approach. The population consisted of 59 bibliographic sources, of which 40 were selected for the sample after applying inclusion and exclusion criteria, which reflected relevant information for the topic. The data collection technique was based on search engines such as Scielo, Pubmed, Latindex, Repositories, Manuals and books. As a result, three standard stains were obtained in the Pathological Anatomy area, which are: Hematoxylin-Eosin staining, Giemsa staining and Papanicolaou staining, in the application of different tissues and cells that reach the laboratory for their respective processing. It was concluded that the Hematoxylin-Eosin stain is the most widely used due to its low cost, requires little instrumentation in its preparation and also that medical specialists in cyto-histopathology use it as a standard Gold technique, while staining with Giemsa is the favorite for the identification of gastric biopsies, based on its good sensitivity and specificity, allowing the identification of cellular characteristics of the gastric mucosa and finally, the Papanicolaou stain is recommended for cytological diagnosis, it is excellent evaluating the characteristics of nuclear chromatin, differential and cytoplasmic transparency, being a success of cervical cytology as a screening method for the detection of cervical cancer. Key words: Stain, Stain, Hematoxylin-Eosin stain, Giemsa stain and Papanicolaou stain Reviewed by: Danilo Yépez Oviedo English professor UNACH 0601574692 |
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