Diagnóstico de laboratorio clínico en la infección humana por amebas de vida libre

ABSTRACT: Free-living amoebas (FLA) are distributed in the environment. They can infect humans, producing primary amoebic meningoencephalitis, amoebic granulomatous encephalitis, keratitis, and cutaneous amebiosis. The research is qualitative, transversal, its objective exploratory, and documentary. Aims to collect information related to the diagnosis of (FLA) from the search and bibliographic analysis of documents contained in biomedical databases: Scielo, Pubmed, Medline, Lilacs, Scopus, BVS, Dialnet, and Google Scholar. Eighty-five documents were depurated until getting a sample of 41, after filtration under the inclusion and exclusion criteria. The most frequent genera are Acanthamoeba and Naegleria fowleri, followed by Balamuthia mandrillaris and Sappinia. The diagnostic techniques used were: Direct Examination, stains with May Grunwald-Giemsa, Hematoxylin-Eosin, PAS, Acridine Orange Fluorescent and White Fluorine Calco, and culture with not nutritious agar media. Besides, use is described of Direct and Indirect Immunofluorescence, Immunoelectrophoresis and Flow Cytometry in immunodiagnosis, although they show low sensitivity. Finally, PCR is included as the choice technique in molecular diagnosis, being considered the most appropriate due to its excellent sensitivity, specificity, and speed, having the advantage of requiring minimal sample quantities. The importance of incorporating the detection of FLA in suspected cases is concluded, to contribute to the timely diagnosis, making possible the application of a proper treatment, which favors reducing patients' mortality. KEY WORDS: Free-living amoebas, Acanthamoeba, Naegleria, Balamuthia, Sappinia diagnosis.

• 1. autor: Jessica Maribel, Tenelema Tenelema (author)
• Format: bachelorThesis
• Język: spa
• Wydane: 2021
• Hasła przedmiotowe: Amebas de vida libre; Acanthamoeba; Naegleria; Balamuthia
• Dostęp online: http://dspace.unach.edu.ec/handle/51000/7396