Caracterización microbiológica y fenotípica de Streptococcus mutans en muestras de saliva.

The main etiologic agent of dental caries is Streptococcus mutans, which stands out especially for its high prevalence in schoolchildren between 5 and 12 years of age. The identification of Streptococcus mutans is important for establishing cariogenic risk and for taking preventive measures. The aim...

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Збережено в:
Бібліографічні деталі
Автор: Andagana Torres, Juan Carlos (author)
Інші автори: Nuñez Ramirez, Carlos Daniel (author)
Формат: bachelorThesis
Мова:spa
Опубліковано: 2024
Предмети:
Онлайн доступ:http://dspace.unach.edu.ec/handle/51000/13210
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Резюме:The main etiologic agent of dental caries is Streptococcus mutans, which stands out especially for its high prevalence in schoolchildren between 5 and 12 years of age. The identification of Streptococcus mutans is important for establishing cariogenic risk and for taking preventive measures. The aim of this research was to investigate the microbiological and phenotypic characteristics of S. mutans present in saliva samples, to understand their clinical implication and possible practical applications to improve oral health focused from the clinical laboratory area. This research was carried out in a documentary, non-experimental, descriptive and qualitative way, with a population and sample of 63 and 34 scientific articles, respectively. These results allow us to have a broad vision about the pathogenicity of S. Mutans, its acidogenicity, aciduricity, its high capacity to form biofilms, among others. In addtion, according to studies, variants of the Mitis salivarius culture medium present a greater specificity for S. Mutans, mainly SB-20M agar, where its colonies are large transparent with a granular appearance similar to ground glass with or without a drop of extracellular polysaccharide. Concerning its enzymatic profile, it presents: positive fermentation of carbohydrates; negative hydrolysis of esculin in presence of bile; negative urease; negative hydrolysis of arginine, and resistance to 2 U of bacitracin. Nowadays, bacterial identification methods go further than conventional methods and use more complex methods such as molecular methods or mass spectrophotometry. Consequently, this study emphasizes the importance of knowing the microbiological and phenotypic characteristics of this bacterium, its pathogenicity and the different methods that can be applied for its identification.