“Determinación de la actividad Hemoaglutinante y bactericida en extractos obtenidos de algas y líquenes adquiridos en la zona de la laguna de Ozogoche en el período junio – octubre del 2016”.

This research project aimed to evaluate the antimicrobial activity in algae and lichen extracts and determine the presence of lectins by performing hemagglutinating activity of algae and lichens. During the period June-October 2016, eight extracts of lichen and four algae we considered: Methanol, Hexane and Aqueous, obtained by dryness, crushing and reflux. The antimicrobial activity was carried out by means of the microdilution method in liquid medium in 96-well plates, using the extracts dissolved in Dimethylsulfoxide (DMSO) and dilutions of these, against American Type Culture Collection (ATCC) microorganisms of: Staphylococcus aureus and Escherichia Coli. For the hemagglutinating activity, the aqueous extract and its dilutions were also used using a 5% suspension of red blood cells (A⁺, B⁺, O⁺). The antimicrobial effect was estimated by the determination of CMB and CMI, while the presence of lectins was assessed according to the intensity of agglutination that the red cells suffered. It is concluded that the extracts at the concentrations tested showed antimicrobial action against the two strains studied being the most active on the growth of Staphylococcus aureus, the lowest activity on Escherichia coli may be due to the composition of its cell wall which is more complex. However, 800 ppm CMB was obtained particularly in the case of Peltigera sp extract, it was also determined that there are lectins being part of the composition of algae and lichens.

• 1. Verfasser: Marcia Alexandra, Cepeda Paca (author)
• Format: bachelorThesis
• Sprache: spa
• Veröffentlicht: 2017
• Schlagworte: Actividad Hemoaglutinante; Algas
• Online Zugang: http://dspace.unach.edu.ec/handle/51000/3734