Genotipos del Virus Papiloma Humano de alto y bajo riesgo en cérvix mediante la Reacción en Cadena de la Polimerasa

Human papillomavirus affects the skin and mucous membranes, including the vaginal tract. Genotyping is used to detect high-risk strains of the virus, which are associated with an elevated chance of developing cancer, whereas low-risk variants can cause genital warts but are rarely related with cancer. This field research was conducted with the aim of collecting different cervicovaginal samples to be analyzed by Polymerase Chain Reaction, detecting high and low risk Human Papillomavirus genotypes. It is a quantitative, exploratory, field and non-experimental study, where samples from 124 women from the Faculty of Health Sciences were analyzed in the period 2023-2S, of which 27 had important cervical lesions, which were analyzed by the Polymerase Chain Reaction technique for the detection of Human Papillomavirus genotypes. With the results, the proposed objectives were achieved, where it was evident that genotype 31 has a higher incidence with 12.50% being considered high oncological risk, while within the low-risk genotypes with a 50% incidence, genotype 43 is present. The samples were analyzed by real-time Polymerase Chain Reaction, based on Tacman techno,th the quantitative fluorescence instrument, which demonstrates a high incidence of positive results for Human papillomavirus, this test being confirmatory for a final diagnosis.

• Main Author: Arellano Espinoza, Gely Odalys (author)
• Other Authors: Marcatoma Guanin, Nathalia Isabel (author)
• Format: bachelorThesis
• Language: spa
• Published: 2024
• Subjects: Virus del Papiloma Humano; Reacción en Cadena de la Polimerasa; Genotipificación; Cervicovaginal; Lesiones cervicales
• Online Access: http://dspace.unach.edu.ec/handle/51000/14225