Diagnóstico de la leishmaniasis por observación microscópica del parásito y detección del ADN

ABSTRACT The following research work had the objective of collecting information on the flagellated parasite of the genus Leishmania by searching and reviewing the literature from primary and secondary sources that can be used to diagnose leishmaniasis by microscopic observation of the parasite and DNA detection. The methodology used in this work was qualitative, documentary, cross-sectional and retrospective, with a sample of 58 useful bibliographic sources closely related to the object of study; Information collected from websites (WHO / PAHO), epidemiological reports, digital books and manuals, and articles from databases such as Scopus, ProQuest, Scielo, Redalyc, Lilacs and Medigraphic, published in the last 10 years to meet the criteria of present and respond to the study problem. To collect the data, an advanced search strategy applied in the same databases and web search engines, keywords selected, and the information filtered with the last 10 years, Spanish and English language, and in the medical and health disciplines. The results obtained were that, for the diagnosis of leishmaniasis in the laboratory, the traditional smear stained with Giemsa or Wright continues to be the gold standard technique, despite presenting a low and variable sensitivity, on the other hand, molecular diagnosis by PCR is highly sensitive and specific for diagnosis because it allowed the specific identification and differentiation of the causative species of leishmaniasis by detecting molecular markers or specific genetic targets in its genome. Keywords: leishmaniasis, laboratory diagnosis, microscopy, DNA, PCR Reviewed by: Mgs. Maritza Chávez Aguagallo ENGLISH PROFESSOR c.c. 0602232324

• Auteur principal: Julio Fabricio, Lunavictoria Beltrán (author)
• Format: bachelorThesis
• Langue: spa
• Publié: 2021
• Sujets: leishmaniasis; diagnóstico de laboratorio; microscopía; ADN
• Accès en ligne: http://dspace.unach.edu.ec/handle/51000/7934