“Actividad biológica de las lectinas obtenidas de semillas del achotillo (Nephelium lappaceum) y uvilla (Physalis peruviana), Riobamba, 2019 - 2020”
The main objective of this research project was to determine the biological activity of the phytoagglutinins (lectins) obtained from the seeds of achotillo (Nephelium lappaceum) and uvilla (Physalis peruviana) in Riobamba, the extract of each fruit was obtained by means of mechanical disruption addi...
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| Eará dahkkit: | |
| Materiálatiipa: | bachelorThesis |
| Giella: | spa |
| Almmustuhtton: |
2020
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| Fáttát: | |
| Liŋkkat: | http://dspace.unach.edu.ec/handle/51000/6800 |
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Lasit fáddágilkoriid | _version_ | 1863456546893070336 |
|---|---|
| author | Castro Cabrera, Jocelyne Ángela |
| author2 | Naspud Villafuerte, Margarita Alexandra |
| author2_role | author |
| author_facet | Castro Cabrera, Jocelyne Ángela Naspud Villafuerte, Margarita Alexandra |
| author_role | author |
| collection | Repositorio Universidad Nacional de Chimborazo |
| dc.contributor.none.fl_str_mv | Pablo Djabayan, Djibeyan |
| dc.creator.none.fl_str_mv | Castro Cabrera, Jocelyne Ángela Naspud Villafuerte, Margarita Alexandra |
| dc.date.none.fl_str_mv | 2020-08-19T04:10:37Z 2020-08-19T04:10:37Z 2020-08-18 |
| dc.format.none.fl_str_mv | 49p. |
| dc.identifier.none.fl_str_mv | Facultad de Ciencias de la Salud 0125-2020 UNACH.FCS.MED http://dspace.unach.edu.ec/handle/51000/6800 |
| dc.language.none.fl_str_mv | spa |
| dc.publisher.none.fl_str_mv | Universidad Nacional de Chimborazo |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by-nc-sa/3.0/ec/ info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Universidad Nacional de Chimborazo instname:Universidad Nacional de Chimborazo instacron:UNACH |
| dc.subject.none.fl_str_mv | Lectinas Semillas de frutas Actividad Biológica Actividad Antimicrobiana |
| dc.title.none.fl_str_mv | “Actividad biológica de las lectinas obtenidas de semillas del achotillo (Nephelium lappaceum) y uvilla (Physalis peruviana), Riobamba, 2019 - 2020” |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
| description | The main objective of this research project was to determine the biological activity of the phytoagglutinins (lectins) obtained from the seeds of achotillo (Nephelium lappaceum) and uvilla (Physalis peruviana) in Riobamba, the extract of each fruit was obtained by means of mechanical disruption adding 15 mL of Physiological Saline Solution, it was incubated, subsequently filtered through a fine nylon fabric and finally centrifuged using a conventional centrifuge to obtain a clear supernatant. The biological activity of the phytoagglutinins was determined evaluating in the aqueous extract of the fruits, the haemagglutinating activity with 0.1 ml of suspension of erythrocytes of blood groups A, B and O, anticoagulant activity on platelet poor plasma with the measurement of TP and aPTT and finally antimicrobial activity with the agar diffusion method using the Kirby Bauer technique on the bacterial strains Escherichia coli, Staphylococcus aureus and Enterococcus faecalis. The results obtained from haemagglutination demonstrated a high ability of lectins to bind red blood cells from blood groups A, B and O, on the other hand, no effect on the coagulation proteins of the extrinsic pathway measured through Prothrombin Time (TP) was established, however, the anticoagulant action on the coagulation proteins of the intrinsic pathway measured through the activated Thromboplastin Partial Time (aPTT) revealed a highly significant prolongation with the achotillo extract, finally the absence of antimicrobial activity by both extracts on the bacterial strains evaluated in this study. Keywords: |
| eu_rights_str_mv | openAccess |
| format | bachelorThesis |
| id | UNACH_be4746c2c4086cdbcf9bf9cbce74a592 |
| identifier_str_mv | Facultad de Ciencias de la Salud 0125-2020 UNACH.FCS.MED |
| instacron_str | UNACH |
| institution | UNACH |
| instname_str | Universidad Nacional de Chimborazo |
| language | spa |
| network_acronym_str | UNACH |
| network_name_str | Repositorio Universidad Nacional de Chimborazo |
| oai_identifier_str | oai:localhost:51000/6800 |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Universidad Nacional de Chimborazo |
| reponame_str | Repositorio Universidad Nacional de Chimborazo |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Repositorio Universidad Nacional de Chimborazo - Universidad Nacional de Chimborazo |
| repository_id_str | 0 |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by-nc-sa/3.0/ec/ |
| spelling | “Actividad biológica de las lectinas obtenidas de semillas del achotillo (Nephelium lappaceum) y uvilla (Physalis peruviana), Riobamba, 2019 - 2020”Castro Cabrera, Jocelyne ÁngelaNaspud Villafuerte, Margarita AlexandraLectinasSemillas de frutasActividad BiológicaActividad AntimicrobianaThe main objective of this research project was to determine the biological activity of the phytoagglutinins (lectins) obtained from the seeds of achotillo (Nephelium lappaceum) and uvilla (Physalis peruviana) in Riobamba, the extract of each fruit was obtained by means of mechanical disruption adding 15 mL of Physiological Saline Solution, it was incubated, subsequently filtered through a fine nylon fabric and finally centrifuged using a conventional centrifuge to obtain a clear supernatant. The biological activity of the phytoagglutinins was determined evaluating in the aqueous extract of the fruits, the haemagglutinating activity with 0.1 ml of suspension of erythrocytes of blood groups A, B and O, anticoagulant activity on platelet poor plasma with the measurement of TP and aPTT and finally antimicrobial activity with the agar diffusion method using the Kirby Bauer technique on the bacterial strains Escherichia coli, Staphylococcus aureus and Enterococcus faecalis. The results obtained from haemagglutination demonstrated a high ability of lectins to bind red blood cells from blood groups A, B and O, on the other hand, no effect on the coagulation proteins of the extrinsic pathway measured through Prothrombin Time (TP) was established, however, the anticoagulant action on the coagulation proteins of the intrinsic pathway measured through the activated Thromboplastin Partial Time (aPTT) revealed a highly significant prolongation with the achotillo extract, finally the absence of antimicrobial activity by both extracts on the bacterial strains evaluated in this study. Keywords:El presente proyecto de investigación planteó como principal objetivo determinar la actividad biológica de las fitoaglutininas (lectinas) obtenidas a partir de las semillas del achotillo (Nephelium lappaceum) y uvilla (Physalis peruviana) en Riobamba, de cada fruta se obtuvo el extracto por medio de disrupción mecánica adicionándole 15 ml de Solución Salina Fisiológica, fue sometido a incubación, posteriormente se filtró a través de un tejido de nylon fino y finalmente se centrifugó utilizando una centrifuga convencional para obtener un sobrenadante claro. La actividad biológica de las fitoaglutininas se determinó evaluando en el extracto acuoso de las frutas, la actividad hemoaglutinante con 0.1 ml de suspensión de eritrocitos de los grupos sanguíneos A, B y O, la actividad anticoagulante sobre el plasma pobre en plaquetas con la medición de TP y TTPa y finalmente la actividad antimicrobiana con método de difusión agar utilizando la técnica de Kirby Bauer sobre las cepas bacterianas Escherichia coli, Staphylococcus aureus y Enterococcus faecalis. Los resultados obtenidos de la hemoaglutinación demostraron una alta capacidad de las lectinas para aglutinar a los eritrocitos de los grupos sanguíneos A, B y O, por otro lado no se evidenció ningún efecto sobre las proteínas de la coagulación de la vía extrínseca medido a través del Tiempo de Protrombina (TP), sin embargo, la acción anticoagulante sobre las proteínas de la coagulación de la vía intrínseca medida a través del Tiempo Parcial de Tromboplastina activado (TTPa) reveló una prolongación altamente significativa en el extracto del achotillo, finalmente se evidenció la ausencia de actividad antimicrobiana por parte de ambos extractos sobre las cepas bacterianas evaluadas en este estudioUNACH,Sede EcuadorUniversidad Nacional de ChimborazoPablo Djabayan, Djibeyan2020-08-19T04:10:37Z2020-08-19T04:10:37Z2020-08-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesis49p.Facultad de Ciencias de la Salud0125-2020 UNACH.FCS.MEDhttp://dspace.unach.edu.ec/handle/51000/6800spahttp://creativecommons.org/licenses/by-nc-sa/3.0/ec/info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Nacional de Chimborazoinstname:Universidad Nacional de Chimborazoinstacron:UNACH2022-06-15T15:56:12Zoai:localhost:51000/6800Institucionalhttp://dspace.unach.edu.ec/Universidad públicahttps://www.unach.edu.ec/http://dspace.unach.edu.ec/oai.Ecuador...opendoar:02022-06-15T15:56:12Repositorio Universidad Nacional de Chimborazo - Universidad Nacional de Chimborazofalse |
| spellingShingle | “Actividad biológica de las lectinas obtenidas de semillas del achotillo (Nephelium lappaceum) y uvilla (Physalis peruviana), Riobamba, 2019 - 2020” Castro Cabrera, Jocelyne Ángela Lectinas Semillas de frutas Actividad Biológica Actividad Antimicrobiana |
| status_str | publishedVersion |
| title | “Actividad biológica de las lectinas obtenidas de semillas del achotillo (Nephelium lappaceum) y uvilla (Physalis peruviana), Riobamba, 2019 - 2020” |
| title_full | “Actividad biológica de las lectinas obtenidas de semillas del achotillo (Nephelium lappaceum) y uvilla (Physalis peruviana), Riobamba, 2019 - 2020” |
| title_fullStr | “Actividad biológica de las lectinas obtenidas de semillas del achotillo (Nephelium lappaceum) y uvilla (Physalis peruviana), Riobamba, 2019 - 2020” |
| title_full_unstemmed | “Actividad biológica de las lectinas obtenidas de semillas del achotillo (Nephelium lappaceum) y uvilla (Physalis peruviana), Riobamba, 2019 - 2020” |
| title_short | “Actividad biológica de las lectinas obtenidas de semillas del achotillo (Nephelium lappaceum) y uvilla (Physalis peruviana), Riobamba, 2019 - 2020” |
| title_sort | “Actividad biológica de las lectinas obtenidas de semillas del achotillo (Nephelium lappaceum) y uvilla (Physalis peruviana), Riobamba, 2019 - 2020” |
| topic | Lectinas Semillas de frutas Actividad Biológica Actividad Antimicrobiana |
| url | http://dspace.unach.edu.ec/handle/51000/6800 |