Actividad biologica de extractos acuosos obtenidos a partir de semillas de achotillo y taxo
ABSTRACT: Bacterial resistance to antibiotics being an increasing public health problem worldwide, the aim of this project was to determine the biological activity of aqueous extracts obtained from the seeds of Taxo (Passiflora tripartita) and Achotillo (Nephelium lappaceum). The aqueous extracts we...
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| Format: | bachelorThesis |
| Język: | spa |
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2023
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| Dostęp online: | http://dspace.unach.edu.ec/handle/51000/11296 |
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| author | Martínez Carrasco, Jonathan Edward |
| author2 | Vela Jiménez, Jhonny Bladimir |
| author2_role | author |
| author_facet | Martínez Carrasco, Jonathan Edward Vela Jiménez, Jhonny Bladimir |
| author_role | author |
| collection | Repositorio Universidad Nacional de Chimborazo |
| dc.contributor.none.fl_str_mv | Djabayan Djibeyan, Pablo |
| dc.creator.none.fl_str_mv | Martínez Carrasco, Jonathan Edward Vela Jiménez, Jhonny Bladimir |
| dc.date.none.fl_str_mv | 2023-07-20T17:31:58Z 2023-07-20T17:31:58Z 2023-07-20 |
| dc.format.none.fl_str_mv | 69p. |
| dc.identifier.none.fl_str_mv | Martínez Carrasco, J - Vela Jiménez, J(2023) Actividad biologica de extractos acuosos obtenidos a partir de semillas de achotillo y taxo. .(Tesis de Pregrado)Universidad Nacional de Chimborazo, Riobamba, Ecuador. UNACH-FCS-MED http://dspace.unach.edu.ec/handle/51000/11296 |
| dc.language.none.fl_str_mv | spa |
| dc.publisher.none.fl_str_mv | Universidad Nocional de Chimborazo |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by-nc-sa/3.0/ec/ info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Universidad Nacional de Chimborazo instname:Universidad Nacional de Chimborazo instacron:UNACH |
| dc.subject.none.fl_str_mv | lectinas hemoaglutinina antibacterianos anticoagulantes |
| dc.title.none.fl_str_mv | Actividad biologica de extractos acuosos obtenidos a partir de semillas de achotillo y taxo |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
| description | ABSTRACT: Bacterial resistance to antibiotics being an increasing public health problem worldwide, the aim of this project was to determine the biological activity of aqueous extracts obtained from the seeds of Taxo (Passiflora tripartita) and Achotillo (Nephelium lappaceum). The aqueous extracts were obtained by mechanical disruption using 15mL of sterile physiological saline. The biological activity of the aqueous extracts was determined by determining their ability to agglutinate suspended erythrocytes of human blood groups A, B, and O, the anticoagulant activity was determined by assessing the activity of the extracts on plasma coagulation proteins in platelet-poor plasma measured by TP and TTPa, the antibacterial activity of the extracts was determined by inhibiting the growth of ATCC bacterial strains. Cytotoxic activity was assessed by determining the lethal dose fifty of the extracts on Artemia salina nauplii. The results revealed that the Taxo extract possesses the ability to agglutinate human erythrocytes non-selectively. Achotillo and Taxo extracts were shown to have anticoagulant action by lengthening TP and TTPa. They also possess antibacterial activity on Staphylococcus aureus strain, while only Taxo extract showed activity against Proteus mirabilis strain. In terms of cytotoxic activity, both extracts were found to be non-toxic. It can be concluded that the seeds of Taxo and Achotillo possess: haemagglutinating, anticoagulant, antibacterial activity, and no cytotoxicity. Keywords: lectins, "biological activity", "haemagglutinin", antibacterial, anticoagulant. |
| eu_rights_str_mv | openAccess |
| format | bachelorThesis |
| id | UNACH_e0fe28fa87fc3b63c287fc132be176cf |
| identifier_str_mv | Martínez Carrasco, J - Vela Jiménez, J(2023) Actividad biologica de extractos acuosos obtenidos a partir de semillas de achotillo y taxo. .(Tesis de Pregrado)Universidad Nacional de Chimborazo, Riobamba, Ecuador. UNACH-FCS-MED |
| instacron_str | UNACH |
| institution | UNACH |
| instname_str | Universidad Nacional de Chimborazo |
| language | spa |
| network_acronym_str | UNACH |
| network_name_str | Repositorio Universidad Nacional de Chimborazo |
| oai_identifier_str | oai:localhost:51000/11296 |
| publishDate | 2023 |
| publisher.none.fl_str_mv | Universidad Nocional de Chimborazo |
| reponame_str | Repositorio Universidad Nacional de Chimborazo |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Repositorio Universidad Nacional de Chimborazo - Universidad Nacional de Chimborazo |
| repository_id_str | 0 |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by-nc-sa/3.0/ec/ |
| spelling | Actividad biologica de extractos acuosos obtenidos a partir de semillas de achotillo y taxoMartínez Carrasco, Jonathan EdwardVela Jiménez, Jhonny BladimirlectinashemoaglutininaantibacterianosanticoagulantesABSTRACT: Bacterial resistance to antibiotics being an increasing public health problem worldwide, the aim of this project was to determine the biological activity of aqueous extracts obtained from the seeds of Taxo (Passiflora tripartita) and Achotillo (Nephelium lappaceum). The aqueous extracts were obtained by mechanical disruption using 15mL of sterile physiological saline. The biological activity of the aqueous extracts was determined by determining their ability to agglutinate suspended erythrocytes of human blood groups A, B, and O, the anticoagulant activity was determined by assessing the activity of the extracts on plasma coagulation proteins in platelet-poor plasma measured by TP and TTPa, the antibacterial activity of the extracts was determined by inhibiting the growth of ATCC bacterial strains. Cytotoxic activity was assessed by determining the lethal dose fifty of the extracts on Artemia salina nauplii. The results revealed that the Taxo extract possesses the ability to agglutinate human erythrocytes non-selectively. Achotillo and Taxo extracts were shown to have anticoagulant action by lengthening TP and TTPa. They also possess antibacterial activity on Staphylococcus aureus strain, while only Taxo extract showed activity against Proteus mirabilis strain. In terms of cytotoxic activity, both extracts were found to be non-toxic. It can be concluded that the seeds of Taxo and Achotillo possess: haemagglutinating, anticoagulant, antibacterial activity, and no cytotoxicity. Keywords: lectins, "biological activity", "haemagglutinin", antibacterial, anticoagulant.RESUMEN: Siendo la resistencia bacteriana a los antibióticos un problema de salud pública en aumento a nivel mundial, el objetivo de este proyecto fue determinar la actividad biológica de los extractos acuosos obtenidos a partir de las semillas del Taxo (Passiflora tripartita) y Achotillo (Nephelium lappaceum). Los extractos acuosos se obtuvieron a través de disrupción mecánica utilizando 15mL de solución salina fisiológica estéril, La actividad biológica de los extractos acuosos se realizó determinando su capacidad de aglutinar de eritrocitos en suspensión de los grupos sanguíneos humanos A, B, y O, la actividad anticoagulante se determinó evaluando la actividad de los extractos sobre las proteínas plasmáticas de la coagulación en un plasma pobre en plaquetas medida mediante el TP y TTPa, la actividad antibacteriana de los extractos se determinó mediante la inhibición del crecimiento de cepas bacterianas ATCC. La actividad citotóxica se evaluó determinando la dosis letal 50 de los extractos sobre nauplios de Artemia salina. Los resultados revelaron que el extracto de Taxo posee la capacidad para aglutinar los eritrocitos humanos no selectivamente. Los extractos del Achotillo y el Taxo mostraron tener acción anticoagulante alargando el TP y el TTPa. También poseen actividad antibacteriana sobre la cepa de Staphylococcus aureus, mientras que solo el extracto de Taxo presentó actividad contra la cepa de Proteus mirabilis. En cuanto a la actividad citotóxica ambos extractos resultaron ser no tóxicos. Se puede concluir indicando que las semillas de Taxo y Achotillo poseen: actividad hemoaglutinante, anticoagulante, antibacteriana y no presentan citotoxicidad.UNACH,EcuadorUniversidad Nocional de ChimborazoDjabayan Djibeyan, Pablo2023-07-20T17:31:58Z2023-07-20T17:31:58Z2023-07-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesis69p.Martínez Carrasco, J - Vela Jiménez, J(2023) Actividad biologica de extractos acuosos obtenidos a partir de semillas de achotillo y taxo. .(Tesis de Pregrado)Universidad Nacional de Chimborazo, Riobamba, Ecuador.UNACH-FCS-MEDhttp://dspace.unach.edu.ec/handle/51000/11296spahttp://creativecommons.org/licenses/by-nc-sa/3.0/ec/info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Nacional de Chimborazoinstname:Universidad Nacional de Chimborazoinstacron:UNACH2023-10-03T08:16:21Zoai:localhost:51000/11296Institucionalhttp://dspace.unach.edu.ec/Universidad públicahttps://www.unach.edu.ec/http://dspace.unach.edu.ec/oai.Ecuador...opendoar:02023-10-03T08:16:21Repositorio Universidad Nacional de Chimborazo - Universidad Nacional de Chimborazofalse |
| spellingShingle | Actividad biologica de extractos acuosos obtenidos a partir de semillas de achotillo y taxo Martínez Carrasco, Jonathan Edward lectinas hemoaglutinina antibacterianos anticoagulantes |
| status_str | publishedVersion |
| title | Actividad biologica de extractos acuosos obtenidos a partir de semillas de achotillo y taxo |
| title_full | Actividad biologica de extractos acuosos obtenidos a partir de semillas de achotillo y taxo |
| title_fullStr | Actividad biologica de extractos acuosos obtenidos a partir de semillas de achotillo y taxo |
| title_full_unstemmed | Actividad biologica de extractos acuosos obtenidos a partir de semillas de achotillo y taxo |
| title_short | Actividad biologica de extractos acuosos obtenidos a partir de semillas de achotillo y taxo |
| title_sort | Actividad biologica de extractos acuosos obtenidos a partir de semillas de achotillo y taxo |
| topic | lectinas hemoaglutinina antibacterianos anticoagulantes |
| url | http://dspace.unach.edu.ec/handle/51000/11296 |