Determinación de la presencia del virus de newcastle e influenza aviar en aves de traspatio de la provincia de el Oro

The Newcastle and Avian Influenza are hidden hazards in commercial poultry which ones must be observed by monitoring in order to prevent losses in this industry. The main objective of this research was to determine the presence of Newcastle (NC) and Avian Influenza (AI) viruses in birds in backyard that are near wetlands and hydrological systems in commercial poultry in El Oro province. Also, 300 backyard -birds were sampled by swab sewage in different areas according to the formula of prevalence limit 1% described by González (1986), taking into account three to four samples per sampling during the period from January to April 2016, with the use of GIS using ArcMap software. Then, through viral isolation in embryonated SPF chicken eggs, was determined by the hemagglutinating allantoic fluid activity, and confirmed by tests of hemagglutination (HA) that 100% of the specimens tested were negative to the virus of Avian Influenza and Newcastle. The technique of evaluation of risk through "epiR" version 09-79 showed that the prevalence of NC and AI virus in backyard poultry and cockfight birds with a prevalence of 0% and a confidence interval of 0 to 1.19%, with a novel of reliability of 95%; so it is concluded that during the period of this research these two diseases virus did not flow, so it is necessary to carry out periodic monitoring, and process samples directly with molecular techniques that enable the identification of small amounts of genetic material RNA concentration of these two virus, and that only RT-qPCR is capable of detecting it (Kubista et al., 2006). Key words: backyard poultry, Newcastle and Avian Influenza, isolation.

• Glavni autor: Salazar Zambrano, Luis Fabricio (author)
• Format: bachelorThesis
• Jezik: spa
• Izdano: 2017
• Teme: VIRUS DE NEWCASTLE; AVES DE TRASPATIO; INFLUENZA AVIAR
• Online pristup: http://dspace.unl.edu.ec/jspui/handle/123456789/19163