Identificación y biocontrol de los microorganismos causantes de la Marchitez Vascular del Babaco (Vasconcellea heilbornii Badillo) procedentes de Loja.
Babaco vascular wilt disease (BVD), caused by a complex of fungi of the genus Fusarium spp. is one of the most economically important diseases. It has caused great devastation in the crop's plantations, leading growers to use chemical control strategies and even to stop the activity due to the...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| التنسيق: | bachelorThesis |
| اللغة: | spa |
| منشور في: |
2024
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://dspace.unl.edu.ec/jspui/handle/123456789/29679 |
| الوسوم: |
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إضافة وسم | _version_ | 1857833069898104832 |
|---|---|
| author | Correa Jiménez, Auliria Francisca |
| author_facet | Correa Jiménez, Auliria Francisca |
| author_role | author |
| collection | Repositorio Universidad Nacional de Loja |
| dc.contributor.none.fl_str_mv | Robles Carrión, Ángel Rolando |
| dc.creator.none.fl_str_mv | Correa Jiménez, Auliria Francisca |
| dc.date.none.fl_str_mv | 2024-04-30T13:44:02Z 2024-04-30T13:44:02Z 2024-04-30 |
| dc.format.none.fl_str_mv | 47 p. application/pdf |
| dc.identifier.none.fl_str_mv | https://dspace.unl.edu.ec/jspui/handle/123456789/29679 |
| dc.language.none.fl_str_mv | spa |
| dc.publisher.none.fl_str_mv | Loja |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by-nc-sa/3.0/ec/ info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Universidad Nacional de Loja instname:Universidad Nacional de Loja instacron:UNL |
| dc.subject.none.fl_str_mv | CARACTERIZACION MORFOLOGICA CARACTERIZACION CULTURAL POTENCIAL DE BIOCONTROLES |
| dc.title.none.fl_str_mv | Identificación y biocontrol de los microorganismos causantes de la Marchitez Vascular del Babaco (Vasconcellea heilbornii Badillo) procedentes de Loja. |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
| description | Babaco vascular wilt disease (BVD), caused by a complex of fungi of the genus Fusarium spp. is one of the most economically important diseases. It has caused great devastation in the crop's plantations, leading growers to use chemical control strategies and even to stop the activity due to the decrease in yields. There is a useful and environmentally friendly control alternative: biological control, which proposes the search for antagonistic microorganisms to control pathogenic microorganisms. The objective of this research was first to characterize the isolates associated with BVM in Loja, for which samples of Babaco roots with symptoms of BVM were collected and characterized morphologically and culturally, taking into account aspects such as: colony pigmentation, mycelial type, measurement of radial growth, spore count, measurement of macro and microconidia. Posteriorly, the in vitro antagonism of Trichoderma spp. Penicillum spp. and Lecanicillum spp. against the 5 isolates of Fusarium spp. was evaluated using a completely randomized design with bifactorial arrangement and the data were statistically processed by testing assumptions of homogeneity and variance by Tukey's tests. The results obtained indicate that the 5 isolates present characteristics corresponding to the genus Fusarium spp. in which variability was observed, reflecting the presence of various species of Fusarium spp. and according to the radial growth rate, two classification categories were identified: 2 isolates with medium growth and 3 with rapid growth. Regarding biocontrol, the results indicate that the strain of Trichoderma spp. showed a high potential of PICR: >50 % of the isolates of Fusarium spp, so it is classified as a good controller. Penicillum spp. and Lecanicillum spp. strains showed a lower potential as biocontrollers. It is concluded that Trichoderma spp. is the best controller, standing out for its efficacy in the inhibition of Fusarium spp. |
| eu_rights_str_mv | openAccess |
| format | bachelorThesis |
| id | UNL_0f40dbec424bcd943a4619d148d8d8d1 |
| instacron_str | UNL |
| institution | UNL |
| instname_str | Universidad Nacional de Loja |
| language | spa |
| network_acronym_str | UNL |
| network_name_str | Repositorio Universidad Nacional de Loja |
| oai_identifier_str | oai:dspace.unl.edu.ec:123456789/29679 |
| publishDate | 2024 |
| publisher.none.fl_str_mv | Loja |
| reponame_str | Repositorio Universidad Nacional de Loja |
| repository.mail.fl_str_mv | * |
| repository.name.fl_str_mv | Repositorio Universidad Nacional de Loja - Universidad Nacional de Loja |
| repository_id_str | 0 |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by-nc-sa/3.0/ec/ |
| spelling | Identificación y biocontrol de los microorganismos causantes de la Marchitez Vascular del Babaco (Vasconcellea heilbornii Badillo) procedentes de Loja.Correa Jiménez, Auliria FranciscaCARACTERIZACION MORFOLOGICACARACTERIZACION CULTURALPOTENCIAL DE BIOCONTROLESBabaco vascular wilt disease (BVD), caused by a complex of fungi of the genus Fusarium spp. is one of the most economically important diseases. It has caused great devastation in the crop's plantations, leading growers to use chemical control strategies and even to stop the activity due to the decrease in yields. There is a useful and environmentally friendly control alternative: biological control, which proposes the search for antagonistic microorganisms to control pathogenic microorganisms. The objective of this research was first to characterize the isolates associated with BVM in Loja, for which samples of Babaco roots with symptoms of BVM were collected and characterized morphologically and culturally, taking into account aspects such as: colony pigmentation, mycelial type, measurement of radial growth, spore count, measurement of macro and microconidia. Posteriorly, the in vitro antagonism of Trichoderma spp. Penicillum spp. and Lecanicillum spp. against the 5 isolates of Fusarium spp. was evaluated using a completely randomized design with bifactorial arrangement and the data were statistically processed by testing assumptions of homogeneity and variance by Tukey's tests. The results obtained indicate that the 5 isolates present characteristics corresponding to the genus Fusarium spp. in which variability was observed, reflecting the presence of various species of Fusarium spp. and according to the radial growth rate, two classification categories were identified: 2 isolates with medium growth and 3 with rapid growth. Regarding biocontrol, the results indicate that the strain of Trichoderma spp. showed a high potential of PICR: >50 % of the isolates of Fusarium spp, so it is classified as a good controller. Penicillum spp. and Lecanicillum spp. strains showed a lower potential as biocontrollers. It is concluded that Trichoderma spp. is the best controller, standing out for its efficacy in the inhibition of Fusarium spp.La enfermedad Marchitez Vascular del Babaco (MVB), ocasionada por el complejo de hongos del género Fusarium spp. es una de las enfermedades de mayor importancia económica. Ha provocado grandes devastaciones en las plantaciones del cultivo, lo que ha llevado a los agricultores a recurrir a estrategias de control químico e incluso a abandonar la actividad debido a la disminución del rendimiento. Existe una alternativa de control útil además de amigable con el ambiente, se trata del control biológico, que propone la búsqueda de microorganismos antagonistas para controlar los microorganismos patógenos. El objetivo de esta investigación consistió primeramente en caracterizar los aislados asociados a la MVB, en Loja, para lo cual se recolectaron muestras de raíz de Babaco con síntomas de MVB y se caracterizaron, morfológica y culturalmente, teniendo en cuenta aspectos como: pigmentación de las colonias, tipo de micelio, medición del crecimiento radial, conteo de esporas, medición de macro y microconidios. Posteriormente, se evaluó el antagonismo in vitro de Trichoderma spp. Penicillium spp. y Lecanicillium spp. contra los 5 aislados de Fusarium spp. para ello se utilizó un diseño completamente al azar con arreglo bifactorial y los datos se procesaron estadísticamente comprobando supuestos de homogeneidad y varianza mediante pruebas de Tukey. Los resultados obtenidos indican que los 5 aislados presentan características correspondientes al género Fusarium spp. en el cual se observó variabilidad, lo que refleja la presencia de diversas especies de Fusarium spp. y según la tasa de crecimiento radial se identificaron dos categorías de clasificación: 2 aislados con crecimiento medio y 3 con crecimiento rápido. Referente al biocontrol los resultados indican que la cepa de Trichoderma spp. mostró un potencial alto de PICR: >50 % de los aislados de Fusarium spp, por lo que se lo clasifica como un buen controlador. Las cepas de Penicillium spp. y Lecanicillium spp. demostraron un potencial menor como biocontroladores. Se concluye que Trichoderma spp. es el mejor controlador, destacando por su eficacia en la inhibición de Fusarium spp.LojaRobles Carrión, Ángel Rolando2024-04-30T13:44:02Z2024-04-30T13:44:02Z2024-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesis47 p.application/pdfhttps://dspace.unl.edu.ec/jspui/handle/123456789/29679spahttp://creativecommons.org/licenses/by-nc-sa/3.0/ec/info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Nacional de Lojainstname:Universidad Nacional de Lojainstacron:UNL2025-05-02T15:54:39Zoai:dspace.unl.edu.ec:123456789/29679Institucionalhttps://dspace.unl.edu.ec/Universidad públicahttps://unl.edu.ec/https://dspace.unl.edu.ec/oaiEcuador***opendoar:02025-05-02T15:54:39falseInstitucionalhttps://dspace.unl.edu.ec/Universidad públicahttps://unl.edu.ec/https://dspace.unl.edu.ec/oai*Ecuador***opendoar:02025-05-02T15:54:39Repositorio Universidad Nacional de Loja - Universidad Nacional de Lojafalse |
| spellingShingle | Identificación y biocontrol de los microorganismos causantes de la Marchitez Vascular del Babaco (Vasconcellea heilbornii Badillo) procedentes de Loja. Correa Jiménez, Auliria Francisca CARACTERIZACION MORFOLOGICA CARACTERIZACION CULTURAL POTENCIAL DE BIOCONTROLES |
| status_str | publishedVersion |
| title | Identificación y biocontrol de los microorganismos causantes de la Marchitez Vascular del Babaco (Vasconcellea heilbornii Badillo) procedentes de Loja. |
| title_full | Identificación y biocontrol de los microorganismos causantes de la Marchitez Vascular del Babaco (Vasconcellea heilbornii Badillo) procedentes de Loja. |
| title_fullStr | Identificación y biocontrol de los microorganismos causantes de la Marchitez Vascular del Babaco (Vasconcellea heilbornii Badillo) procedentes de Loja. |
| title_full_unstemmed | Identificación y biocontrol de los microorganismos causantes de la Marchitez Vascular del Babaco (Vasconcellea heilbornii Badillo) procedentes de Loja. |
| title_short | Identificación y biocontrol de los microorganismos causantes de la Marchitez Vascular del Babaco (Vasconcellea heilbornii Badillo) procedentes de Loja. |
| title_sort | Identificación y biocontrol de los microorganismos causantes de la Marchitez Vascular del Babaco (Vasconcellea heilbornii Badillo) procedentes de Loja. |
| topic | CARACTERIZACION MORFOLOGICA CARACTERIZACION CULTURAL POTENCIAL DE BIOCONTROLES |
| url | https://dspace.unl.edu.ec/jspui/handle/123456789/29679 |